Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).

Antifungal resistance is increasing by the emergence of intrinsically resistant species and by the development of secondary resistance in susceptible species. A previous study performed in Spain revealed levels of azole resistance in molds of between 10 and 12.7%, but secondary resistance in Aspergi...

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Autores: Ayats-Ardite, Josefina, Castro, Carmen, García-Rodríguez, Julio, Goterris-Bonet, Lidia, Ibáñez-Martínez, Elisa, Linares-Sicilia, María José, Martín-Gómez, María Teresa, Martín-Mazuelos, Estrella, Pelaez, Teresa, Pemán, Javier, Rezusta, Antonio, Rojo, Susana, Tejero, Rocío, Anza, Diego Vicente, Viñuelas, Jesús, Zapico, Maria Soledad, Alastruey-Izquierdo, Ana, Alcazar-Fuoli, Laura, Rivero-Menendez, Olga, Cuenca-Estrella, Manuel
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/10340
Acceso en línea:http://hdl.handle.net/20.500.12105/10340
Access Level:acceso abierto
Palabra clave:Antifungal Agents
Aspergillus fumigatus
Cytochrome P-450 Enzyme System
Drug Resistance, Fungal
Fungal Proteins
Humans
Itraconazole
Microbial Sensitivity Tests
Prospective Studies
Spain
Triazoles
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spelling Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).Ayats-Ardite, JosefinaCastro, CarmenGarcía-Rodríguez, JulioGoterris-Bonet, LidiaIbáñez-Martínez, ElisaLinares-Sicilia, María JoséMartín-Gómez, María TeresaMartín-Mazuelos, EstrellaPelaez, TeresaPemán, JavierRezusta, AntonioRojo, SusanaTejero, RocíoAnza, Diego VicenteViñuelas, JesúsZapico, Maria SoledadAlastruey-Izquierdo, AnaAlcazar-Fuoli, LauraRivero-Menendez, OlgaCuenca-Estrella, ManuelAntifungal AgentsAspergillus fumigatusCytochrome P-450 Enzyme SystemDrug Resistance, FungalFungal ProteinsHumansItraconazoleMicrobial Sensitivity TestsProspective StudiesSpainTriazolesAntifungal resistance is increasing by the emergence of intrinsically resistant species and by the development of secondary resistance in susceptible species. A previous study performed in Spain revealed levels of azole resistance in molds of between 10 and 12.7%, but secondary resistance in Aspergillus fumigatus was not detected. We used itraconazole (ITZ)-supplemented medium to select resistant strains. A total of 500 plates supplemented with 2 mg/liter of ITZ were sent to 10 Spanish tertiary hospitals, and molecular identification and antifungal susceptibility testing were performed. In addition, the cyp51A gene in those A. fumigatus strains showing azole resistance was sequenced. A total of 493 isolates were included in the study. Sixteen strains were isolated from patients with an infection classified as proven, 104 were isolated from patients with an infection classified as probable, and 373 were isolated from patients with an infection classified as colonization. Aspergillus was the most frequent genus isolated, at 80.3%, followed by Scedosporium-Lomentospora (7.9%), Penicillium-Talaromyces (4.5%), Fusarium (2.6%), and the order Mucorales (1%). Antifungal resistance was detected in Scedosporium-Lomentospora species, Fusarium, Talaromyces, and Mucorales Three strains of A. fumigatus sensu stricto were resistant to azoles; two of them harbored the TR34+L98H mechanism of resistance, and the other one had no mutations in cyp51A The level of azole resistance in A. fumigatus remains low, but cryptic species represent over 10% of the isolates and have a broader but overall higher range of antifungal resistance.American Society for Microbiology (ASM)Instituto de Salud Carlos III20202020-06-1120182018-01-0120182018-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/10340reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/103402026-06-12T12:43:37Z
dc.title.none.fl_str_mv Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
title Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
spellingShingle Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
Ayats-Ardite, Josefina
Antifungal Agents
Aspergillus fumigatus
Cytochrome P-450 Enzyme System
Drug Resistance, Fungal
Fungal Proteins
Humans
Itraconazole
Microbial Sensitivity Tests
Prospective Studies
Spain
Triazoles
title_short Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
title_full Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
title_fullStr Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
title_full_unstemmed Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
title_sort Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
dc.creator.none.fl_str_mv Ayats-Ardite, Josefina
Castro, Carmen
García-Rodríguez, Julio
Goterris-Bonet, Lidia
Ibáñez-Martínez, Elisa
Linares-Sicilia, María José
Martín-Gómez, María Teresa
Martín-Mazuelos, Estrella
Pelaez, Teresa
Pemán, Javier
Rezusta, Antonio
Rojo, Susana
Tejero, Rocío
Anza, Diego Vicente
Viñuelas, Jesús
Zapico, Maria Soledad
Alastruey-Izquierdo, Ana
Alcazar-Fuoli, Laura
Rivero-Menendez, Olga
Cuenca-Estrella, Manuel
author Ayats-Ardite, Josefina
author_facet Ayats-Ardite, Josefina
Castro, Carmen
García-Rodríguez, Julio
Goterris-Bonet, Lidia
Ibáñez-Martínez, Elisa
Linares-Sicilia, María José
Martín-Gómez, María Teresa
Martín-Mazuelos, Estrella
Pelaez, Teresa
Pemán, Javier
Rezusta, Antonio
Rojo, Susana
Tejero, Rocío
Anza, Diego Vicente
Viñuelas, Jesús
Zapico, Maria Soledad
Alastruey-Izquierdo, Ana
Alcazar-Fuoli, Laura
Rivero-Menendez, Olga
Cuenca-Estrella, Manuel
author_role author
author2 Castro, Carmen
García-Rodríguez, Julio
Goterris-Bonet, Lidia
Ibáñez-Martínez, Elisa
Linares-Sicilia, María José
Martín-Gómez, María Teresa
Martín-Mazuelos, Estrella
Pelaez, Teresa
Pemán, Javier
Rezusta, Antonio
Rojo, Susana
Tejero, Rocío
Anza, Diego Vicente
Viñuelas, Jesús
Zapico, Maria Soledad
Alastruey-Izquierdo, Ana
Alcazar-Fuoli, Laura
Rivero-Menendez, Olga
Cuenca-Estrella, Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
dc.subject.none.fl_str_mv Antifungal Agents
Aspergillus fumigatus
Cytochrome P-450 Enzyme System
Drug Resistance, Fungal
Fungal Proteins
Humans
Itraconazole
Microbial Sensitivity Tests
Prospective Studies
Spain
Triazoles
topic Antifungal Agents
Aspergillus fumigatus
Cytochrome P-450 Enzyme System
Drug Resistance, Fungal
Fungal Proteins
Humans
Itraconazole
Microbial Sensitivity Tests
Prospective Studies
Spain
Triazoles
description Antifungal resistance is increasing by the emergence of intrinsically resistant species and by the development of secondary resistance in susceptible species. A previous study performed in Spain revealed levels of azole resistance in molds of between 10 and 12.7%, but secondary resistance in Aspergillus fumigatus was not detected. We used itraconazole (ITZ)-supplemented medium to select resistant strains. A total of 500 plates supplemented with 2 mg/liter of ITZ were sent to 10 Spanish tertiary hospitals, and molecular identification and antifungal susceptibility testing were performed. In addition, the cyp51A gene in those A. fumigatus strains showing azole resistance was sequenced. A total of 493 isolates were included in the study. Sixteen strains were isolated from patients with an infection classified as proven, 104 were isolated from patients with an infection classified as probable, and 373 were isolated from patients with an infection classified as colonization. Aspergillus was the most frequent genus isolated, at 80.3%, followed by Scedosporium-Lomentospora (7.9%), Penicillium-Talaromyces (4.5%), Fusarium (2.6%), and the order Mucorales (1%). Antifungal resistance was detected in Scedosporium-Lomentospora species, Fusarium, Talaromyces, and Mucorales Three strains of A. fumigatus sensu stricto were resistant to azoles; two of them harbored the TR34+L98H mechanism of resistance, and the other one had no mutations in cyp51A The level of azole resistance in A. fumigatus remains low, but cryptic species represent over 10% of the isolates and have a broader but overall higher range of antifungal resistance.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01
2018
2018-01-01
2020
2020-06-11
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/10340
url http://hdl.handle.net/20.500.12105/10340
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology (ASM)
publisher.none.fl_str_mv American Society for Microbiology (ASM)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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