Formulation of double nanoemulsions based on pH-sensitive poly acrylic acid/agarose/ZnO for quercetin controlled release
Quercetin (QC) is a promising cancer drug, though its poor solubility and quick release reduce its bioavailability, thus limiting its current applications. In this research, a new hydrogel nanocomposite comprising zinc oxide nanoparticles (ZnONPs), agarose, and polyacrylic acid (PAA), was designed t...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/60913 |
| Acceso en línea: | http://hdl.handle.net/10017/60913 https://dx.doi.org/10.1016/j.molliq.2023.123363 |
| Access Level: | acceso abierto |
| Palabra clave: | Quercetin Double nanoemulsions ZnO nanoparticles pH-sensitive nanocarrier Controlled releasePoly acrylic acid Agarose Química Chemistry |
| Sumario: | Quercetin (QC) is a promising cancer drug, though its poor solubility and quick release reduce its bioavailability, thus limiting its current applications. In this research, a new hydrogel nanocomposite comprising zinc oxide nanoparticles (ZnONPs), agarose, and polyacrylic acid (PAA), was designed to load QC. Spherical nanocarriers were prepared via environmentally friendly and simple double emulsion approach by injecting PAA/Aga/ZnONPs coated by SPAN 80 surfactant to a hydrophobic olive oil phase. The nanoemulsions were characterized by different techniques to assess the impact of ZnONPs on PAA/Aga pH-sensitive hydrogel as a novel platform for direct administration of QC. FTIR and XRD analysis confirmed the presence of all the nanocomposite components in the final formulation. FE-SEM images revealed the spherical shape and surface homogeneity of the nanocarriers, and zeta potential measurements corroborated their colloidal stability. The addition of ZnONPS increased the drug loading from 41.25% to 47.50% and the encapsulation efficiency from 83.0% to 87.25%. A slower drug release was observed at pH 7.4 for periods of time shorter than 96 h, corroborating the pH-sensitivity of the nanoemulsions. Drug release data at pH 5.4 fitted well to the First-order equation, while at pH 7.4 were better described by the Korsmeyer Peppas model. The reduction in MCF7 living cells in the presence of PAA/Aga/ZnONPs compared to PAA/Aga and the control sample indicates the in vitro cytotoxicity of ZnONPs. The number of late apoptotic cells in PAA/Aga/ZnONPs/QC (37.55%) was higher than that of the other formulations (ZnONPs, PAA/Aga and PAA/Aga/ZnONPs), which may be due to the controlled and slow release of QC from PAA/Aga/ZnONPs/QC. Therefore, these green, biocompatible and biodegradable nanoemulsions show great potential as nanocarriers for QC loading and sustained release against breast cancer. |
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