Analysis of the dynamic co-expression network of heart regeneration in the zebrafish

The zebrafish has the capacity to regenerate its heart after severe injury. While the function of a few genes during this process has been studied, we are far from fully understanding how genes interact to coordinate heart regeneration. To enable systematic insights into this phenomenon, we generate...

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Bibliographic Details
Authors: Rodius, Sophie, Androsova, Ganna, Gotz, Lou, Liechti, Robin, Crespo, Isaac, Merz, Susanne, Nazarov, Petr V., de Klein, Niek, Jeanty, Celine, Gonzalez-Rosa, Juan M., Muller, Arnaud, Bernardin, Francois, Niclou, Simone P., Vallar, Laurent, Mercader, Nadia, Ibberson, Mark, Xenarios, Ioannis, Azuaje, Francisco
Format: article
Publication Date:2016
Country:España
Institution:Instituto de Salud Carlos III (ISCIII)
Repository:Repisalud
Language:English
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/5228
Online Access:http://hdl.handle.net/20.500.12105/5228
Access Level:Open access
Keyword:MYOCARDIAL-INFARCTION
GENE-EXPRESSION
CARDIAC REGENERATION
EPICARDIAL CELLS
INJURY
CARDIOMYOCYTES
MODEL
CRYOINJURY
TARGET
DIFFERENTIATION
Description
Summary:The zebrafish has the capacity to regenerate its heart after severe injury. While the function of a few genes during this process has been studied, we are far from fully understanding how genes interact to coordinate heart regeneration. To enable systematic insights into this phenomenon, we generated and integrated a dynamic co-expression network of heart regeneration in the zebrafish and linked systems-level properties to the underlying molecular events. Across multiple post-injury time points, the network displays topological attributes of biological relevance. We show that regeneration steps are mediated by modules of transcriptionally coordinated genes, and by genes acting as network hubs. We also established direct associations between hubs and validated drivers of heart regeneration with murine and human orthologs. The resulting models and interactive analysis tools are available at http://infused.vital-it.ch. Using a worked example, we demonstrate the usefulness of this unique open resource for hypothesis generation and in silico screening for genes involved in heart regeneration.

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