Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres

Epigallocatechin gallate (EGCG), the main catechin of green tea, is described to have potential health benefits in several fields like oncology, neurology or cardiology. Currently, it is also under pre-clinical investigation as a potential therapeutic or preventive treatment during pregnancy against...

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Autores: Kühne, Britta A., Puig i Miquel, Teresa, Ruiz-Martínez, Santiago, Crous-Masó, Joan, Planas, Marta, Feliu, Lidia, Cano Fernández, Amanda, García, Maria Luisa, Fritsche, Ellen, Llobet Mallafré, Joan M. (Joan Maria), Gómez Catalán, Jesús, Barenys Espadaler, Marta
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/167697
Acceso en línea:https://hdl.handle.net/2445/167697
Access Level:acceso abierto
Palabra clave:Suplements nutritius
Neurotoxicologia
Embriologia
Dietary supplements
Neurotoxicology
Embryology
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spelling Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat NeurospheresKühne, Britta A.Puig i Miquel, TeresaRuiz-Martínez, SantiagoCrous-Masó, JoanPlanas, MartaFeliu, LidiaCano Fernández, AmandaGarcía, Maria LuisaFritsche, EllenLlobet Mallafré, Joan M. (Joan Maria)Gómez Catalán, JesúsBarenys Espadaler, MartaSuplements nutritiusNeurotoxicologiaEmbriologiaDietary supplementsNeurotoxicologyEmbryologyEpigallocatechin gallate (EGCG), the main catechin of green tea, is described to have potential health benefits in several fields like oncology, neurology or cardiology. Currently, it is also under pre-clinical investigation as a potential therapeutic or preventive treatment during pregnancy against developmental adverse effects induced by toxic substances. However, the safety of EGCG during pregnancy is unclear due to its proven adverse effects on neural progenitor cells' (NPCs) migration. As lately several strategies have arisen to generate new therapeutic agents derived from EGCG, we have used the rat 'Neurosphere Assay' to characterize and compare the effects of EGCG structurally related compounds and EGCG PEGylated PLGA nanoparticles on a neurodevelopmental key event: NPCs migration. Compounds structurally-related to EGCG induce the same pattern of NPCs migration alterations (decreased migration distance, decreased formation of migration corona, chaotic orientation of cellular processes and decreased migration of neurons at higher concentrations). The potency of the compounds does not depend on the number of galloyl groups, and small structure variations can imply large potency differences. Due to their lower toxicity observed in vitro in NPCs, 4,4′-bis[(3,4,5-trihydroxybenzoyl)oxy]-1,1′-biphenyl and EGCG PEGylated PLGA nanoparticles are suggested as potential future therapeutic or preventive alternatives to EGCG during prenatal period​Elsevier Ltd2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/167697Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.fct.2018.10.055Food and Chemical Toxicology, 2019, vol. 123, p. 195-204https://doi.org/10.1016/j.fct.2018.10.055cc-by-nc-nd (c) Elsevier Ltd, 2019http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1676972026-05-27T06:46:51Z
dc.title.none.fl_str_mv Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
title Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
spellingShingle Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
Kühne, Britta A.
Suplements nutritius
Neurotoxicologia
Embriologia
Dietary supplements
Neurotoxicology
Embryology
title_short Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
title_full Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
title_fullStr Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
title_full_unstemmed Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
title_sort Comparison of Migration Disturbance Potency of Epigallocatechin Gallate (EGCG) Synthetic Analogs and EGCG PEGylated PLGA Nanoparticles in Rat Neurospheres
dc.creator.none.fl_str_mv Kühne, Britta A.
Puig i Miquel, Teresa
Ruiz-Martínez, Santiago
Crous-Masó, Joan
Planas, Marta
Feliu, Lidia
Cano Fernández, Amanda
García, Maria Luisa
Fritsche, Ellen
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
Barenys Espadaler, Marta
author Kühne, Britta A.
author_facet Kühne, Britta A.
Puig i Miquel, Teresa
Ruiz-Martínez, Santiago
Crous-Masó, Joan
Planas, Marta
Feliu, Lidia
Cano Fernández, Amanda
García, Maria Luisa
Fritsche, Ellen
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
Barenys Espadaler, Marta
author_role author
author2 Puig i Miquel, Teresa
Ruiz-Martínez, Santiago
Crous-Masó, Joan
Planas, Marta
Feliu, Lidia
Cano Fernández, Amanda
García, Maria Luisa
Fritsche, Ellen
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
Barenys Espadaler, Marta
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Suplements nutritius
Neurotoxicologia
Embriologia
Dietary supplements
Neurotoxicology
Embryology
topic Suplements nutritius
Neurotoxicologia
Embriologia
Dietary supplements
Neurotoxicology
Embryology
description Epigallocatechin gallate (EGCG), the main catechin of green tea, is described to have potential health benefits in several fields like oncology, neurology or cardiology. Currently, it is also under pre-clinical investigation as a potential therapeutic or preventive treatment during pregnancy against developmental adverse effects induced by toxic substances. However, the safety of EGCG during pregnancy is unclear due to its proven adverse effects on neural progenitor cells' (NPCs) migration. As lately several strategies have arisen to generate new therapeutic agents derived from EGCG, we have used the rat 'Neurosphere Assay' to characterize and compare the effects of EGCG structurally related compounds and EGCG PEGylated PLGA nanoparticles on a neurodevelopmental key event: NPCs migration. Compounds structurally-related to EGCG induce the same pattern of NPCs migration alterations (decreased migration distance, decreased formation of migration corona, chaotic orientation of cellular processes and decreased migration of neurons at higher concentrations). The potency of the compounds does not depend on the number of galloyl groups, and small structure variations can imply large potency differences. Due to their lower toxicity observed in vitro in NPCs, 4,4′-bis[(3,4,5-trihydroxybenzoyl)oxy]-1,1′-biphenyl and EGCG PEGylated PLGA nanoparticles are suggested as potential future therapeutic or preventive alternatives to EGCG during prenatal period​
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/167697
url https://hdl.handle.net/2445/167697
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.fct.2018.10.055
Food and Chemical Toxicology, 2019, vol. 123, p. 195-204
https://doi.org/10.1016/j.fct.2018.10.055
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier Ltd, 2019
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier Ltd, 2019
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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