In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms

Candida spp. are one of the most common fungal pathogens. Biofilms formed by Candida albicans offer resistance mechanisms against most antifungal agents. Therefore, development of new molecules effective against these microorganisms, alone or in combination with antifungal drugs, is extremely necess...

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Autores: Heredero Bermejo, Irene|||0000-0001-6984-1965, Gómez Casanova, Natalia|||0000-0002-6983-1895, Quintana Sánchez, Sara|||0000-0003-3184-716X, Soliveri de Carranza, Juan|||0000-0001-8200-0132, Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935, Pérez Serrano, Jorge|||0000-0002-4870-8978, Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285, Copa Patiño, José Luis|||0000-0002-2162-3907
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/59698
Acceso en línea:http://hdl.handle.net/10017/59698
https://dx.doi.org/10.3390/pharmaceutics12100918
Access Level:acceso abierto
Palabra clave:Candida
antifungal drugs
Candida albicans
biofilm
biocides
carbosilane dendrimers
cytotoxicity
scanning electron microscopy
Química
Chemistry
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spelling In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans BiofilmsHeredero Bermejo, Irene|||0000-0001-6984-1965Gómez Casanova, Natalia|||0000-0002-6983-1895Quintana Sánchez, Sara|||0000-0003-3184-716XSoliveri de Carranza, Juan|||0000-0001-8200-0132Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935Pérez Serrano, Jorge|||0000-0002-4870-8978Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285Copa Patiño, José Luis|||0000-0002-2162-3907Candidaantifungal drugsCandida albicansbiofilmbiocidescarbosilane dendrimerscytotoxicityscanning electron microscopyQuímicaChemistryCandida spp. are one of the most common fungal pathogens. Biofilms formed by Candida albicans offer resistance mechanisms against most antifungal agents. Therefore, development of new molecules effective against these microorganisms, alone or in combination with antifungal drugs, is extremely necessary. In the present work, we carried out a screening process of different cationic carbosilane dendritic molecules against C. albicans. In vitro activity against biofilm formation and biofilms was tested in both Coleccion Espanola de Cultivos Tipo (CECT) 1002 and clinical C. albicans strains. Cytotoxicity was studied in human cell lines, and biofilm alterations were observed by scanning electron microscopy (SEM). Antifungal activity of the carbosilane dendritic molecules was assessed by monitoring cell viability using both established and novel cell viability assays. One out of 14 dendritic molecules tested, named BDSQ024, showed the highest activity with a minimum biofilm inhibitory concentration (MBIC) for biofilm formation and a minimum biofilm damaging concentration (MBDC) for existing biofilm of 16-32 and 16 mg/L, respectively. Synergy with amphotericin (AmB) and caspofungin (CSF) at non-cytotoxic concentrations was found. Therefore, dendritic compounds are exciting new antifungals effective at preventing Candida biofilm formation and represent a potential novel therapeutic agent for treatment of C. albicans infection in combination with existing clinical antifungals.Ministerio de Economía, Industria y CompetitividadComunidad de MadridUniversidad de Alcalá20202020-09-21journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/59698https://dx.doi.org/10.3390/pharmaceutics12100918reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available TQ2017-86224-PComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703UAH Not available CCG19%2FCCS-022open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/596982026-06-18T11:13:07Z
dc.title.none.fl_str_mv In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
title In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
spellingShingle In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
Heredero Bermejo, Irene|||0000-0001-6984-1965
Candida
antifungal drugs
Candida albicans
biofilm
biocides
carbosilane dendrimers
cytotoxicity
scanning electron microscopy
Química
Chemistry
title_short In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
title_full In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
title_fullStr In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
title_full_unstemmed In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
title_sort In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
dc.creator.none.fl_str_mv Heredero Bermejo, Irene|||0000-0001-6984-1965
Gómez Casanova, Natalia|||0000-0002-6983-1895
Quintana Sánchez, Sara|||0000-0003-3184-716X
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Pérez Serrano, Jorge|||0000-0002-4870-8978
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Copa Patiño, José Luis|||0000-0002-2162-3907
author Heredero Bermejo, Irene|||0000-0001-6984-1965
author_facet Heredero Bermejo, Irene|||0000-0001-6984-1965
Gómez Casanova, Natalia|||0000-0002-6983-1895
Quintana Sánchez, Sara|||0000-0003-3184-716X
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Pérez Serrano, Jorge|||0000-0002-4870-8978
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Copa Patiño, José Luis|||0000-0002-2162-3907
author_role author
author2 Gómez Casanova, Natalia|||0000-0002-6983-1895
Quintana Sánchez, Sara|||0000-0003-3184-716X
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Pérez Serrano, Jorge|||0000-0002-4870-8978
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Copa Patiño, José Luis|||0000-0002-2162-3907
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Candida
antifungal drugs
Candida albicans
biofilm
biocides
carbosilane dendrimers
cytotoxicity
scanning electron microscopy
Química
Chemistry
topic Candida
antifungal drugs
Candida albicans
biofilm
biocides
carbosilane dendrimers
cytotoxicity
scanning electron microscopy
Química
Chemistry
description Candida spp. are one of the most common fungal pathogens. Biofilms formed by Candida albicans offer resistance mechanisms against most antifungal agents. Therefore, development of new molecules effective against these microorganisms, alone or in combination with antifungal drugs, is extremely necessary. In the present work, we carried out a screening process of different cationic carbosilane dendritic molecules against C. albicans. In vitro activity against biofilm formation and biofilms was tested in both Coleccion Espanola de Cultivos Tipo (CECT) 1002 and clinical C. albicans strains. Cytotoxicity was studied in human cell lines, and biofilm alterations were observed by scanning electron microscopy (SEM). Antifungal activity of the carbosilane dendritic molecules was assessed by monitoring cell viability using both established and novel cell viability assays. One out of 14 dendritic molecules tested, named BDSQ024, showed the highest activity with a minimum biofilm inhibitory concentration (MBIC) for biofilm formation and a minimum biofilm damaging concentration (MBDC) for existing biofilm of 16-32 and 16 mg/L, respectively. Synergy with amphotericin (AmB) and caspofungin (CSF) at non-cytotoxic concentrations was found. Therefore, dendritic compounds are exciting new antifungals effective at preventing Candida biofilm formation and represent a potential novel therapeutic agent for treatment of C. albicans infection in combination with existing clinical antifungals.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-09-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/59698
https://dx.doi.org/10.3390/pharmaceutics12100918
url http://hdl.handle.net/10017/59698
https://dx.doi.org/10.3390/pharmaceutics12100918
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available TQ2017-86224-P
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703
UAH Not available CCG19%2FCCS-022
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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