PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk

PVOH-based polymer matrices in the form of films were evaluated as carriers of living Lactococcus lactis subsp. Lactis. These lactic acid bacteria are capable of producing nisin, which is an effective antilisterial peptide. A low percentage (1:0.125 w/w) of yeast extract, gelatin, sodium caseinate,...

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Detalles Bibliográficos
Autores: Settier Ramírez, Laura, López-Carballo, Gracia, Gavara, Rafael, Hernández-Muñoz, Pilar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/386431
Acceso en línea:http://hdl.handle.net/10261/386431
https://api.elsevier.com/content/abstract/scopus_id/85079835606
Access Level:acceso abierto
Palabra clave:Lactococcus lactis
Listeria monocytogenes
PVOH films
Pasteurized milk
Protein
Protein hydrolysates
pasteurized milk
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spelling PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milkSettier Ramírez, LauraLópez-Carballo, GraciaGavara, RafaelHernández-Muñoz, PilarLactococcus lactisListeria monocytogenesPVOH filmsPasteurized milkProteinProtein hydrolysatesLactococcus lactisListeria monocytogenespasteurized milkPVOH-based polymer matrices in the form of films were evaluated as carriers of living Lactococcus lactis subsp. Lactis. These lactic acid bacteria are capable of producing nisin, which is an effective antilisterial peptide. A low percentage (1:0.125 w/w) of yeast extract, gelatin, sodium caseinate, gelatin, or casein hydrolysates was incorporated in PVOH matrices with the aim of increasing the viability of bacteria in the film. The films were obtained by casting after incorporating L. lactis. Then they were evaluated for antilisterial activity in liquid medium at 37 °C for 24 h, and also at 4 °C for 21 days in order to simulate the storage of liquid foods in refrigeration conditions. The survival of the lactic acid bacteria was also evaluated at both temperatures during the experiment. L. lactis remained viable in all the films tested at 37 and 4 °C. The antimicrobial activity of the films was greater at 4 °C than at 37 °C. With regard to the effect of the film composition, the activity of the films was higher when protein hydrolysates and sodium caseinate were incorporated in the formulation. Films supplemented with protein hydrolysates or sodium caseinate inhibited growth of the pathogen during the 21 days of storage at 4 °C. At 37 °C, after 24 h the films had slowed the growth of the inoculated pathogen by between 2 and 4 log CFU/mL. Finally, as the films developed are intended to be used in the design of active packaging of foods, they were tested in pasteurized milk inoculated with 4 log CFU/mL of Listeria monocytogenes and stored at 4 °C for 21 days. The pathogen began to grow after the second day of storage with or without film, but when the films were added to the medium the growth of the pathogen was slowed down, without reaching >6 log CFU, whereas the control reached a maximum growth of 8.5 log CFU. The pH of the milk was monitored throughout the experiment, and it decreased with time. This was due to the generation of organic acids by the lactic bacteria. Buffering the food stabilized the pH without modifying the activity of the films. Thus, the current study shows that PVOH films supplemented with nutrients can act as carriers of L. lactis, and they can help to increase the safety of refrigerated dairy beverages and sauces.The authors acknowledge the financial support of the Spanish Ministry of Economy and Competitiveness (AGL2015-64595-R).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Settier Ramírez, Laura [0000-0001-8500-9650]Gavara, Rafael [0000-0001-6376-8939]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/386431https://api.elsevier.com/content/abstract/scopus_id/85079835606reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/Ministerio de Economía y Competitividad/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64595-RInternational journal of food microbiologyhttps://doi.org/10.1016/j.ijfoodmicro.2020.108545Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3864312026-05-22T06:33:51Z
dc.title.none.fl_str_mv PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
title PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
spellingShingle PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
Settier Ramírez, Laura
Lactococcus lactis
Listeria monocytogenes
PVOH films
Pasteurized milk
Protein
Protein hydrolysates
Lactococcus lactis
Listeria monocytogenes
pasteurized milk
title_short PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
title_full PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
title_fullStr PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
title_full_unstemmed PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
title_sort PVOH/protein blend films embedded with lactic acid bacteria and their antilisterial activity in pasteurized milk
dc.creator.none.fl_str_mv Settier Ramírez, Laura
López-Carballo, Gracia
Gavara, Rafael
Hernández-Muñoz, Pilar
author Settier Ramírez, Laura
author_facet Settier Ramírez, Laura
López-Carballo, Gracia
Gavara, Rafael
Hernández-Muñoz, Pilar
author_role author
author2 López-Carballo, Gracia
Gavara, Rafael
Hernández-Muñoz, Pilar
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Settier Ramírez, Laura [0000-0001-8500-9650]
Gavara, Rafael [0000-0001-6376-8939]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lactococcus lactis
Listeria monocytogenes
PVOH films
Pasteurized milk
Protein
Protein hydrolysates
Lactococcus lactis
Listeria monocytogenes
pasteurized milk
topic Lactococcus lactis
Listeria monocytogenes
PVOH films
Pasteurized milk
Protein
Protein hydrolysates
Lactococcus lactis
Listeria monocytogenes
pasteurized milk
description PVOH-based polymer matrices in the form of films were evaluated as carriers of living Lactococcus lactis subsp. Lactis. These lactic acid bacteria are capable of producing nisin, which is an effective antilisterial peptide. A low percentage (1:0.125 w/w) of yeast extract, gelatin, sodium caseinate, gelatin, or casein hydrolysates was incorporated in PVOH matrices with the aim of increasing the viability of bacteria in the film. The films were obtained by casting after incorporating L. lactis. Then they were evaluated for antilisterial activity in liquid medium at 37 °C for 24 h, and also at 4 °C for 21 days in order to simulate the storage of liquid foods in refrigeration conditions. The survival of the lactic acid bacteria was also evaluated at both temperatures during the experiment. L. lactis remained viable in all the films tested at 37 and 4 °C. The antimicrobial activity of the films was greater at 4 °C than at 37 °C. With regard to the effect of the film composition, the activity of the films was higher when protein hydrolysates and sodium caseinate were incorporated in the formulation. Films supplemented with protein hydrolysates or sodium caseinate inhibited growth of the pathogen during the 21 days of storage at 4 °C. At 37 °C, after 24 h the films had slowed the growth of the inoculated pathogen by between 2 and 4 log CFU/mL. Finally, as the films developed are intended to be used in the design of active packaging of foods, they were tested in pasteurized milk inoculated with 4 log CFU/mL of Listeria monocytogenes and stored at 4 °C for 21 days. The pathogen began to grow after the second day of storage with or without film, but when the films were added to the medium the growth of the pathogen was slowed down, without reaching >6 log CFU, whereas the control reached a maximum growth of 8.5 log CFU. The pH of the milk was monitored throughout the experiment, and it decreased with time. This was due to the generation of organic acids by the lactic bacteria. Buffering the food stabilized the pH without modifying the activity of the films. Thus, the current study shows that PVOH films supplemented with nutrients can act as carriers of L. lactis, and they can help to increase the safety of refrigerated dairy beverages and sauces.
publishDate 2020
dc.date.none.fl_str_mv 2020
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/386431
https://api.elsevier.com/content/abstract/scopus_id/85079835606
url http://hdl.handle.net/10261/386431
https://api.elsevier.com/content/abstract/scopus_id/85079835606
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/Ministerio de Economía y Competitividad/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64595-R
International journal of food microbiology
https://doi.org/10.1016/j.ijfoodmicro.2020.108545
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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