Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation

[EN] Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a pot...

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Autores: Valente, Angela H., Roode, Matthew de, Ernst, Madeleine, Peña Espinoza, Miguel, Bornancin, Louis, Bonde, Charlotte S., Martínez Valladares, María, Ramünke, Sabrina, Krücken, Jürgen, Simonsen, Henrik T., Thamsborg, Stig M., Williams, Andrew R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18882
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S2211320721000051?via%3Dihub
https://hdl.handle.net/10612/18882
Access Level:acceso abierto
Palabra clave:Sanidad animal
Chicory
Anthelmintic
Parasite
Sesquiterpene lactones
3109 Ciencias Veterinarias
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spelling Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionationValente, Angela H.Roode, Matthew deErnst, MadeleinePeña Espinoza, MiguelBornancin, LouisBonde, Charlotte S.Martínez Valladares, MaríaRamünke, SabrinaKrücken, JürgenSimonsen, Henrik T.Thamsborg, Stig M.Williams, Andrew R.Sanidad animalChicoryAnthelminticParasiteSesquiterpene lactones3109 Ciencias Veterinarias[EN] Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a potentially useful source for novel anthelmintic compounds or a diet-based preventive/therapeutic option. Here, we utilized in vitro bioassays with the parasitic nematode Ascaris suum and molecular networking techniques with five chicory cultivars to identify putative active compounds. Network analysis predicted sesquiterpene lactones (SL) as the most likely group of anthelmintic compounds. Further bioassay-guided fractionation supported these predictions, and isolation of pure compounds demonstrated that the SL 8-deoxylactucin (8-DOL) is the compound most strongly associated with anti-parasitic activity. Furthermore, we showed that 8-DOL acts in a synergistic combination with other SL to exert the anti-parasitic effects. Finally, we established that chicory-derived extracts also showed activity against two ruminant nematodes (Teladorsagia circumcincta and Cooperia oncophora) in in vitro assays. Collectively, our results confirm the anti-parasitic activity of chicory against a range of nematodes, and pave the way for targeted extraction of active compounds or selective breeding of specific cultivars to optimize its future use in human and veterinary medicineSIWe are very grateful for the guidance and support by Dr. Thomas Ostenfeld Larsen and Christopher Phippen, Technical University of Denmark, Natural product discovery, and the laboratory assistance of Mette Schjelde, University of Copenhagen. This work was funded by the Danish Council for Independent Research (Grant DFF–6111-00394). Fractionation and purification of compounds were further supported by the Green Development and Demonstration Program (GUDP) (Project No. 34009-17-1220). MPE was supported by CONICYT Chile (FONDECYT Postdoctorado #3170875)ElsevierSanidad AnimalFacultad de Veterinaria2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.sciencedirect.com/science/article/pii/S2211320721000051?via%3Dihubhttps://hdl.handle.net/10612/18882reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosIngléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/188822026-06-24T12:43:27Z
dc.title.none.fl_str_mv Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
spellingShingle Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
Valente, Angela H.
Sanidad animal
Chicory
Anthelmintic
Parasite
Sesquiterpene lactones
3109 Ciencias Veterinarias
title_short Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_full Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_fullStr Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_full_unstemmed Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
title_sort Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
dc.creator.none.fl_str_mv Valente, Angela H.
Roode, Matthew de
Ernst, Madeleine
Peña Espinoza, Miguel
Bornancin, Louis
Bonde, Charlotte S.
Martínez Valladares, María
Ramünke, Sabrina
Krücken, Jürgen
Simonsen, Henrik T.
Thamsborg, Stig M.
Williams, Andrew R.
author Valente, Angela H.
author_facet Valente, Angela H.
Roode, Matthew de
Ernst, Madeleine
Peña Espinoza, Miguel
Bornancin, Louis
Bonde, Charlotte S.
Martínez Valladares, María
Ramünke, Sabrina
Krücken, Jürgen
Simonsen, Henrik T.
Thamsborg, Stig M.
Williams, Andrew R.
author_role author
author2 Roode, Matthew de
Ernst, Madeleine
Peña Espinoza, Miguel
Bornancin, Louis
Bonde, Charlotte S.
Martínez Valladares, María
Ramünke, Sabrina
Krücken, Jürgen
Simonsen, Henrik T.
Thamsborg, Stig M.
Williams, Andrew R.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Chicory
Anthelmintic
Parasite
Sesquiterpene lactones
3109 Ciencias Veterinarias
topic Sanidad animal
Chicory
Anthelmintic
Parasite
Sesquiterpene lactones
3109 Ciencias Veterinarias
description [EN] Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a potentially useful source for novel anthelmintic compounds or a diet-based preventive/therapeutic option. Here, we utilized in vitro bioassays with the parasitic nematode Ascaris suum and molecular networking techniques with five chicory cultivars to identify putative active compounds. Network analysis predicted sesquiterpene lactones (SL) as the most likely group of anthelmintic compounds. Further bioassay-guided fractionation supported these predictions, and isolation of pure compounds demonstrated that the SL 8-deoxylactucin (8-DOL) is the compound most strongly associated with anti-parasitic activity. Furthermore, we showed that 8-DOL acts in a synergistic combination with other SL to exert the anti-parasitic effects. Finally, we established that chicory-derived extracts also showed activity against two ruminant nematodes (Teladorsagia circumcincta and Cooperia oncophora) in in vitro assays. Collectively, our results confirm the anti-parasitic activity of chicory against a range of nematodes, and pave the way for targeted extraction of active compounds or selective breeding of specific cultivars to optimize its future use in human and veterinary medicine
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S2211320721000051?via%3Dihub
https://hdl.handle.net/10612/18882
url https://www.sciencedirect.com/science/article/pii/S2211320721000051?via%3Dihub
https://hdl.handle.net/10612/18882
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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