High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics

Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surfa...

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Authors: Tatkiewicz, Witold I.|||0000-0003-1349-2174, Seras-Franzoso, Joaquin|||0000-0002-7893-4773, Garcia-Fruitos, Elena|||0000-0001-7498-4864, Vázquez, Esther|||0000-0003-1052-0424, Kyvik, Adriana R.|||0000-0002-6385-7162, Ventosa, Nora|||0000-0002-8008-4974, Guasch, Judith|||0000-0002-3571-4711, Villaverde, Antonio|||0000-0002-2615-4521, Veciana i Miró, Jaume|||0000-0003-1023-9923, Ratera Bastardas, Imma|||0000-0002-1464-9789
Format: article
Publication Date:2019
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:233716
Online Access:https://ddd.uab.cat/record/233716
https://dx.doi.org/urn:doi:10.1021/acsbiomaterials.9b01085
Access Level:Open access
Keyword:Inclusion Bodies
Cell motility
High throughput
Tissue engineering
Concentration gradients
Surface patterning
Protein nanoparticles
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spelling High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristicsTatkiewicz, Witold I.|||0000-0003-1349-2174Seras-Franzoso, Joaquin|||0000-0002-7893-4773Garcia-Fruitos, Elena|||0000-0001-7498-4864Vázquez, Esther|||0000-0003-1052-0424Kyvik, Adriana R.|||0000-0002-6385-7162Ventosa, Nora|||0000-0002-8008-4974Guasch, Judith|||0000-0002-3571-4711Villaverde, Antonio|||0000-0002-2615-4521Veciana i Miró, Jaume|||0000-0003-1023-9923Ratera Bastardas, Imma|||0000-0002-1464-9789Inclusion BodiesCell motilityHigh throughputTissue engineeringConcentration gradientsSurface patterningProtein nanoparticlesEighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enabled to perform a high-throughput study of the motility of NIH-3T3 fibroblasts under different conditions including the gradient steepness, particle concentrations, and area widths of patterned FGF-IBs, using for the data analysis a methodology that includes "heat maps". From this analysis, we observed that gradients of concentrations of surface-bound FGF-IBs stimulate the total cell movement but do not affect the total net distances traveled by cells. Moreover, cells tend to move toward an optimal intermediate FGF-IB concentration (i.e., cells seeded on areas with high IB concentrations moved toward areas with lower concentrations and vice versa, reaching the optimal concentration). Additionally, a higher motility was obtained when cells were deposited on narrow and highly concentrated areas with IBs. FGF-IBs can be therefore used to enhance and guide cell migration, confirming that the decoration of surfaces with such IB-like protein nanoparticles is a promising platform for regenerative medicine and tissue engineering.Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/233716https://dx.doi.org/urn:doi:10.1021/acsbiomaterials.9b01085reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 MAT2016-80826-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 MAT2013-50036EXPAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-918Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-229European Commission https://doi.org/10.13039/501100000780 654360European Commission https://doi.org/10.13039/501100000780 600388Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 TECSPR15-1-0015Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0496open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2337162026-06-06T12:50:31Z
dc.title.none.fl_str_mv High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
title High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
spellingShingle High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
Tatkiewicz, Witold I.|||0000-0003-1349-2174
Inclusion Bodies
Cell motility
High throughput
Tissue engineering
Concentration gradients
Surface patterning
Protein nanoparticles
title_short High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
title_full High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
title_fullStr High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
title_full_unstemmed High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
title_sort High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
dc.creator.none.fl_str_mv Tatkiewicz, Witold I.|||0000-0003-1349-2174
Seras-Franzoso, Joaquin|||0000-0002-7893-4773
Garcia-Fruitos, Elena|||0000-0001-7498-4864
Vázquez, Esther|||0000-0003-1052-0424
Kyvik, Adriana R.|||0000-0002-6385-7162
Ventosa, Nora|||0000-0002-8008-4974
Guasch, Judith|||0000-0002-3571-4711
Villaverde, Antonio|||0000-0002-2615-4521
Veciana i Miró, Jaume|||0000-0003-1023-9923
Ratera Bastardas, Imma|||0000-0002-1464-9789
author Tatkiewicz, Witold I.|||0000-0003-1349-2174
author_facet Tatkiewicz, Witold I.|||0000-0003-1349-2174
Seras-Franzoso, Joaquin|||0000-0002-7893-4773
Garcia-Fruitos, Elena|||0000-0001-7498-4864
Vázquez, Esther|||0000-0003-1052-0424
Kyvik, Adriana R.|||0000-0002-6385-7162
Ventosa, Nora|||0000-0002-8008-4974
Guasch, Judith|||0000-0002-3571-4711
Villaverde, Antonio|||0000-0002-2615-4521
Veciana i Miró, Jaume|||0000-0003-1023-9923
Ratera Bastardas, Imma|||0000-0002-1464-9789
author_role author
author2 Seras-Franzoso, Joaquin|||0000-0002-7893-4773
Garcia-Fruitos, Elena|||0000-0001-7498-4864
Vázquez, Esther|||0000-0003-1052-0424
Kyvik, Adriana R.|||0000-0002-6385-7162
Ventosa, Nora|||0000-0002-8008-4974
Guasch, Judith|||0000-0002-3571-4711
Villaverde, Antonio|||0000-0002-2615-4521
Veciana i Miró, Jaume|||0000-0003-1023-9923
Ratera Bastardas, Imma|||0000-0002-1464-9789
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia
dc.subject.none.fl_str_mv Inclusion Bodies
Cell motility
High throughput
Tissue engineering
Concentration gradients
Surface patterning
Protein nanoparticles
topic Inclusion Bodies
Cell motility
High throughput
Tissue engineering
Concentration gradients
Surface patterning
Protein nanoparticles
description Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enabled to perform a high-throughput study of the motility of NIH-3T3 fibroblasts under different conditions including the gradient steepness, particle concentrations, and area widths of patterned FGF-IBs, using for the data analysis a methodology that includes "heat maps". From this analysis, we observed that gradients of concentrations of surface-bound FGF-IBs stimulate the total cell movement but do not affect the total net distances traveled by cells. Moreover, cells tend to move toward an optimal intermediate FGF-IB concentration (i.e., cells seeded on areas with high IB concentrations moved toward areas with lower concentrations and vice versa, reaching the optimal concentration). Additionally, a higher motility was obtained when cells were deposited on narrow and highly concentrated areas with IBs. FGF-IBs can be therefore used to enhance and guide cell migration, confirming that the decoration of surfaces with such IB-like protein nanoparticles is a promising platform for regenerative medicine and tissue engineering.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/233716
https://dx.doi.org/urn:doi:10.1021/acsbiomaterials.9b01085
url https://ddd.uab.cat/record/233716
https://dx.doi.org/urn:doi:10.1021/acsbiomaterials.9b01085
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 MAT2016-80826-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 MAT2013-50036EXP
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-918
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-229
European Commission https://doi.org/10.13039/501100000780 654360
European Commission https://doi.org/10.13039/501100000780 600388
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 TECSPR15-1-0015
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0496
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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