Quantitative analysis of the seminal plasma proteome in secondary hypogonadism

In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory...

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Authors: Grande, Giuseppe, Vincenzoni, Federica, Mancini, Francesca, Barrachina, Ferran, Giampietro, Antonella, Castagnola, Massimo, Urbani, Andrea, Oliva Virgili, Rafael, Milardi, Domenico, Pontecorvi, Alfredo
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176317
Online Access:https://hdl.handle.net/2445/176317
Access Level:Open access
Keyword:Testosterona
Proteòmica
Hipogonadisme
Testosterone
Proteomics
Hypogonadism
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spelling Quantitative analysis of the seminal plasma proteome in secondary hypogonadismGrande, GiuseppeVincenzoni, FedericaMancini, FrancescaBarrachina, FerranGiampietro, AntonellaCastagnola, MassimoUrbani, AndreaOliva Virgili, RafaelMilardi, DomenicoPontecorvi, AlfredoTestosteronaProteòmicaHipogonadismeTestosteroneProteomicsHypogonadismIn the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.MDPI2021202120192021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/176317Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/jcm8122128Journal of Clinical Medicine, 2019, vol. 8, num. 12, p. 2128https://doi.org/10.3390/jcm8122128cc-by (c) Grande, Giuseppe et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1763172026-05-29T05:05:01Z
dc.title.none.fl_str_mv Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
title Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
spellingShingle Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
Grande, Giuseppe
Testosterona
Proteòmica
Hipogonadisme
Testosterone
Proteomics
Hypogonadism
title_short Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
title_full Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
title_fullStr Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
title_full_unstemmed Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
title_sort Quantitative analysis of the seminal plasma proteome in secondary hypogonadism
dc.creator.none.fl_str_mv Grande, Giuseppe
Vincenzoni, Federica
Mancini, Francesca
Barrachina, Ferran
Giampietro, Antonella
Castagnola, Massimo
Urbani, Andrea
Oliva Virgili, Rafael
Milardi, Domenico
Pontecorvi, Alfredo
author Grande, Giuseppe
author_facet Grande, Giuseppe
Vincenzoni, Federica
Mancini, Francesca
Barrachina, Ferran
Giampietro, Antonella
Castagnola, Massimo
Urbani, Andrea
Oliva Virgili, Rafael
Milardi, Domenico
Pontecorvi, Alfredo
author_role author
author2 Vincenzoni, Federica
Mancini, Francesca
Barrachina, Ferran
Giampietro, Antonella
Castagnola, Massimo
Urbani, Andrea
Oliva Virgili, Rafael
Milardi, Domenico
Pontecorvi, Alfredo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Testosterona
Proteòmica
Hipogonadisme
Testosterone
Proteomics
Hypogonadism
topic Testosterona
Proteòmica
Hipogonadisme
Testosterone
Proteomics
Hypogonadism
description In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.
publishDate 2019
dc.date.none.fl_str_mv 2019
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176317
url https://hdl.handle.net/2445/176317
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/jcm8122128
Journal of Clinical Medicine, 2019, vol. 8, num. 12, p. 2128
https://doi.org/10.3390/jcm8122128
dc.rights.none.fl_str_mv cc-by (c) Grande, Giuseppe et al., 2019
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Grande, Giuseppe et al., 2019
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408148232601600
score 15,811543

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