Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts

[EN] Plum pox virus (PPV)-D and PPV-R are two isolates from strain D of PPV that differ in host specificity. Previous analyses of chimeras originating from PPV-R and PPV-D suggested that the N terminus of the coat protein (CP) includes host-specific pathogenicity determinants. Here, these determinan...

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Autores: CARBONELL, ALBERTO|||0000-0001-5628-6632, Maliogka, Varvara I., Pérez, José de Jesús, Salvador, Beatriz, San Leon, David, Garcia, Juan Antonio, Simon-Mateo, Carmen
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/160981
Acceso en línea:https://riunet.upv.es/handle/10251/160981
Access Level:acceso abierto
Palabra clave:Ppv
Coat protein
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repository_id_str
spelling Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hostsCARBONELL, ALBERTO|||0000-0001-5628-6632Maliogka, Varvara I.Pérez, José de JesúsSalvador, BeatrizSan Leon, DavidGarcia, Juan AntonioSimon-Mateo, CarmenPpvCoat protein[EN] Plum pox virus (PPV)-D and PPV-R are two isolates from strain D of PPV that differ in host specificity. Previous analyses of chimeras originating from PPV-R and PPV-D suggested that the N terminus of the coat protein (CP) includes host-specific pathogenicity determinants. Here, these determinants were mapped precisely by analyzing the infectivity in herbaceous and woody species of chimeras containing a fragment of the 3' region of PPV-D (including the region coding for the CP) in a PPV-R backbone. These chimeras were not infectious in Prunus persica, but systemically infected Nicotiazza clevelandii and N. benthamiana when specific amino acids were modified or deleted in a short 30-amino-acid region of the N terminus of the CP. Most of these mutations did not reduce PPV fitness in Prunus spp. although others impaired systemic infection in this host. We propose a model in which the N terminus of the CP, highly relevant for virus systemic movement, is targeted by a host defense mechanism in Nicotiana spp. Mutations in this short region allow PPV to overcome the defense response in this host but can compromise the efficiency of PPV systemic movement in other hosts such as Prunus sppThis work was supported by grants BIO2010-18541 from Spanish MEC (Ministry of Economy and Competitiveness), SAL/0185/2006 from Comunidad de Madrid, and KBBE-204429 from the European Union. B. Salvador was a recipient of an FPI (Training of Research Personnel) fellowship from MEC. A. Carbonell and V. I. Maliogka contributed equally to this work. We thank N. Fahlgren (Donald Danforth Plant Science Center) for the review of the English text and B. Garcia and E. Dominguez for technical assistanceScientific SocietiesInstituto Universitario Mixto de Biología Molecular y Celular de PlantasMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20132013-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/160981reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2010-18541 FACTORES DE LA INTERACCION PLANTA-VIRUS RELEVANTES PARA EL CONTROL DEL VIRUS DE LA SHARKA Y PARA SU USO COMO HERRAMIENTA BIOTECNOLOGICAEuropean Commission https://doi.org/10.13039/501100000780 FP7 204429 Sharka Containmentopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1609812026-06-13T07:49:27Z
dc.title.none.fl_str_mv Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
title Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
spellingShingle Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
CARBONELL, ALBERTO|||0000-0001-5628-6632
Ppv
Coat protein
title_short Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
title_full Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
title_fullStr Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
title_full_unstemmed Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
title_sort Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts
dc.creator.none.fl_str_mv CARBONELL, ALBERTO|||0000-0001-5628-6632
Maliogka, Varvara I.
Pérez, José de Jesús
Salvador, Beatriz
San Leon, David
Garcia, Juan Antonio
Simon-Mateo, Carmen
author CARBONELL, ALBERTO|||0000-0001-5628-6632
author_facet CARBONELL, ALBERTO|||0000-0001-5628-6632
Maliogka, Varvara I.
Pérez, José de Jesús
Salvador, Beatriz
San Leon, David
Garcia, Juan Antonio
Simon-Mateo, Carmen
author_role author
author2 Maliogka, Varvara I.
Pérez, José de Jesús
Salvador, Beatriz
San Leon, David
Garcia, Juan Antonio
Simon-Mateo, Carmen
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Ministerio de Ciencia e Innovación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Ppv
Coat protein
topic Ppv
Coat protein
description [EN] Plum pox virus (PPV)-D and PPV-R are two isolates from strain D of PPV that differ in host specificity. Previous analyses of chimeras originating from PPV-R and PPV-D suggested that the N terminus of the coat protein (CP) includes host-specific pathogenicity determinants. Here, these determinants were mapped precisely by analyzing the infectivity in herbaceous and woody species of chimeras containing a fragment of the 3' region of PPV-D (including the region coding for the CP) in a PPV-R backbone. These chimeras were not infectious in Prunus persica, but systemically infected Nicotiazza clevelandii and N. benthamiana when specific amino acids were modified or deleted in a short 30-amino-acid region of the N terminus of the CP. Most of these mutations did not reduce PPV fitness in Prunus spp. although others impaired systemic infection in this host. We propose a model in which the N terminus of the CP, highly relevant for virus systemic movement, is targeted by a host defense mechanism in Nicotiana spp. Mutations in this short region allow PPV to overcome the defense response in this host but can compromise the efficiency of PPV systemic movement in other hosts such as Prunus spp
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/160981
url https://riunet.upv.es/handle/10251/160981
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2010-18541 FACTORES DE LA INTERACCION PLANTA-VIRUS RELEVANTES PARA EL CONTROL DEL VIRUS DE LA SHARKA Y PARA SU USO COMO HERRAMIENTA BIOTECNOLOGICA
European Commission https://doi.org/10.13039/501100000780 FP7 204429 Sharka Containment
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Scientific Societies
publisher.none.fl_str_mv Scientific Societies
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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