A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids
Background: Light, the driving force of photosynthesis, can be harmful when present in excess; therefore, any light harvesting system requires photoprotection. Members of the extended light-harvesting complex (LHC) protein superfamily are involved in light harvesting as well as in photoprotection an...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/23211 |
| Acceso en línea: | http://hdl.handle.net/10230/23211 http://dx.doi.org/10.1186/1471-2148-13-159 |
| Access Level: | acceso abierto |
| Palabra clave: | Seqüència d&apos aminoàcids Proteïnes -- Metabolisme Complex plastids Diatoms Chloroplast Gene transfer Light-harvesting antenna proteins Red lineage chlorophyll a/b-binding-like proteins |
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A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastidsSturm, SabineEngelken, JohannesGruber, AnsgarVugrinec, SaschaKroth, Peter GAdamska, IwonaLavaud, JohannSeqüència d&aposaminoàcidsProteïnes -- MetabolismeComplex plastidsDiatomsChloroplastGene transferLight-harvesting antenna proteinsRed lineage chlorophyll a/b-binding-like proteinsBackground: Light, the driving force of photosynthesis, can be harmful when present in excess; therefore, any light harvesting system requires photoprotection. Members of the extended light-harvesting complex (LHC) protein superfamily are involved in light harvesting as well as in photoprotection and are found in the red and green plant lineages, with a complex distribution pattern of subfamilies in the different algal lineages. Results: Here, we demonstrate that the recently discovered “red lineage chlorophyll a/b-binding-like proteins” (RedCAPs) form a monophyletic family within this protein superfamily. The occurrence of RedCAPs was found to be restricted to the red algal lineage, including red algae (with primary plastids) as well as cryptophytes, haptophytes and heterokontophytes (with secondary plastids of red algal origin). Expression of a full-length RedCAP:GFP fusion construct in the diatom Phaeodactylum tricornutum confirmed the predicted plastid localisation of RedCAPs. Furthermore, we observed that similarly to the fucoxanthin chlorophyll a/c-binding light-harvesting antenna proteins also RedCAP transcripts in diatoms were regulated in a diurnal way at standard light conditions and strongly repressed at high light intensities./nConclusions: The absence of RedCAPs from the green lineage implies that RedCAPs evolved in the red lineage after separation from the the green lineage. During the evolution of secondary plastids, RedCAP genes therefore must have been transferred from the nucleus of the endocytobiotic alga to the nucleus of the host cell, a process that involved complementation with pre-sequences allowing import of the gene product into the secondary plastid bound by four membranes. Based on light-dependent transcription and on localisation data, we propose that RedCAPs might participate in the light (intensity and quality)-dependent structural or functional reorganisation of the light-harvesting antennae of the photosystems upon dark to light shifts as regularly experienced by diatoms in nature. Remarkably, in plastids of the red lineage as well as in green lineage plastids, the phycobilisome based cyanobacterial light harvesting system has been replaced by light harvesting systems that are based on members of the extended LHC protein superfamily, either for one of the photosystems (PS I of red algae) or for both (diatoms). In their proposed function, the RedCAP protein family may thus have played a role in the evolutionary structural remodelling of light-harvesting antennae in the red lineage.This work was supported by the Universität Konstanz and by grants of the Deutsche Forschungsgemeinschaft (KR 1661/3 to PGK, AD 92/7-2 to IA, LA 2368/2-1 to JL). JE was supported by a grant (I/82 750) from the Volkswagenstiftung (“Förderungsinitiative Evolutionsbiologie”)BioMed Central201520152013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/23211http://dx.doi.org/10.1186/1471-2148-13-159reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésBMC Evolutionary Biology. 2013; 13: 159© 2013 Sturm et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/2.0info:eu-repo/semantics/openAccessoai:recercat.cat:10230/232112026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids |
| title |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids |
| spellingShingle |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids Sturm, Sabine Seqüència d&apos aminoàcids Proteïnes -- Metabolisme Complex plastids Diatoms Chloroplast Gene transfer Light-harvesting antenna proteins Red lineage chlorophyll a/b-binding-like proteins |
| title_short |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids |
| title_full |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids |
| title_fullStr |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids |
| title_full_unstemmed |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids |
| title_sort |
A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastids |
| dc.creator.none.fl_str_mv |
Sturm, Sabine Engelken, Johannes Gruber, Ansgar Vugrinec, Sascha Kroth, Peter G Adamska, Iwona Lavaud, Johann |
| author |
Sturm, Sabine |
| author_facet |
Sturm, Sabine Engelken, Johannes Gruber, Ansgar Vugrinec, Sascha Kroth, Peter G Adamska, Iwona Lavaud, Johann |
| author_role |
author |
| author2 |
Engelken, Johannes Gruber, Ansgar Vugrinec, Sascha Kroth, Peter G Adamska, Iwona Lavaud, Johann |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Seqüència d&apos aminoàcids Proteïnes -- Metabolisme Complex plastids Diatoms Chloroplast Gene transfer Light-harvesting antenna proteins Red lineage chlorophyll a/b-binding-like proteins |
| topic |
Seqüència d&apos aminoàcids Proteïnes -- Metabolisme Complex plastids Diatoms Chloroplast Gene transfer Light-harvesting antenna proteins Red lineage chlorophyll a/b-binding-like proteins |
| description |
Background: Light, the driving force of photosynthesis, can be harmful when present in excess; therefore, any light harvesting system requires photoprotection. Members of the extended light-harvesting complex (LHC) protein superfamily are involved in light harvesting as well as in photoprotection and are found in the red and green plant lineages, with a complex distribution pattern of subfamilies in the different algal lineages. Results: Here, we demonstrate that the recently discovered “red lineage chlorophyll a/b-binding-like proteins” (RedCAPs) form a monophyletic family within this protein superfamily. The occurrence of RedCAPs was found to be restricted to the red algal lineage, including red algae (with primary plastids) as well as cryptophytes, haptophytes and heterokontophytes (with secondary plastids of red algal origin). Expression of a full-length RedCAP:GFP fusion construct in the diatom Phaeodactylum tricornutum confirmed the predicted plastid localisation of RedCAPs. Furthermore, we observed that similarly to the fucoxanthin chlorophyll a/c-binding light-harvesting antenna proteins also RedCAP transcripts in diatoms were regulated in a diurnal way at standard light conditions and strongly repressed at high light intensities./nConclusions: The absence of RedCAPs from the green lineage implies that RedCAPs evolved in the red lineage after separation from the the green lineage. During the evolution of secondary plastids, RedCAP genes therefore must have been transferred from the nucleus of the endocytobiotic alga to the nucleus of the host cell, a process that involved complementation with pre-sequences allowing import of the gene product into the secondary plastid bound by four membranes. Based on light-dependent transcription and on localisation data, we propose that RedCAPs might participate in the light (intensity and quality)-dependent structural or functional reorganisation of the light-harvesting antennae of the photosystems upon dark to light shifts as regularly experienced by diatoms in nature. Remarkably, in plastids of the red lineage as well as in green lineage plastids, the phycobilisome based cyanobacterial light harvesting system has been replaced by light harvesting systems that are based on members of the extended LHC protein superfamily, either for one of the photosystems (PS I of red algae) or for both (diatoms). In their proposed function, the RedCAP protein family may thus have played a role in the evolutionary structural remodelling of light-harvesting antennae in the red lineage. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013 2015 2015 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/23211 http://dx.doi.org/10.1186/1471-2148-13-159 |
| url |
http://hdl.handle.net/10230/23211 http://dx.doi.org/10.1186/1471-2148-13-159 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
BMC Evolutionary Biology. 2013; 13: 159 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/2.0 info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/2.0 |
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openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
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BioMed Central |
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reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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