In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer

Our understanding of the circadian clock function in plants has been markedly assisted by studies with the model species Arabidopsis thaliana. Molecular and genetics approaches have delivered a comprehensive view of the transcriptional regulatory networks underlying the Arabidopsis circadian system....

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Detalles Bibliográficos
Autores: Okada, Masaaki, Mas, Paloma|||0000-0002-3780-8041
Tipo de recurso: capítulo de libro
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:259303
Acceso en línea:https://ddd.uab.cat/record/259303
https://dx.doi.org/urn:doi:10.1007/978-1-0716-2249-0_27
Access Level:acceso abierto
Palabra clave:Circadian rhythms
Luciferase
Promoter activity
Gene expression
Arabidopsis thaliana
Bioluminescence
Reporter assays
Microplate luminometer
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spelling In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometerOkada, MasaakiMas, Paloma|||0000-0002-3780-8041Circadian rhythmsLuciferasePromoter activityGene expressionArabidopsis thalianaBioluminescenceReporter assaysMicroplate luminometerOur understanding of the circadian clock function in plants has been markedly assisted by studies with the model species Arabidopsis thaliana. Molecular and genetics approaches have delivered a comprehensive view of the transcriptional regulatory networks underlying the Arabidopsis circadian system. The use of the luciferase as a reporter allowed the precise in vivo determination of circadian periods, phases, and amplitudes of clock promoter activities with unprecedented temporal resolution. An increasing repertoire of fine-tuned luciferases together with additional applications such as translational fusions or bioluminescence molecular complementation assays have considerably expanded our view of circadian protein expression and activity, far beyond transcriptional regulation. Further applications have focused on the in vivo simultaneous examination of rhythms in different parts of the plant. The use of intact versus excised plant organs has also provided a glimpse on both the organ-specific and autonomy of the clocks and the importance of long distance communication for circadian function. This chapter provides a basic protocol for in vivo high-throughput monitoring of circadian rhythms in Arabidopsis seedlings using bioluminescent reporters and a microplate luminometer.Humana 22022-01-0120222022-01-01Capítol de llibrehttp://purl.org/coar/resource_type/c_3248AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/bookPartapplication/pdfhttps://ddd.uab.cat/record/259303https://dx.doi.org/urn:doi:10.1007/978-1-0716-2249-0_27reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-106653GB-I00Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CEX2019-000902-Sopen accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2593032026-06-06T12:50:31Z
dc.title.none.fl_str_mv In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
title In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
spellingShingle In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
Okada, Masaaki
Circadian rhythms
Luciferase
Promoter activity
Gene expression
Arabidopsis thaliana
Bioluminescence
Reporter assays
Microplate luminometer
title_short In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
title_full In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
title_fullStr In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
title_full_unstemmed In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
title_sort In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer
dc.creator.none.fl_str_mv Okada, Masaaki
Mas, Paloma|||0000-0002-3780-8041
author Okada, Masaaki
author_facet Okada, Masaaki
Mas, Paloma|||0000-0002-3780-8041
author_role author
author2 Mas, Paloma|||0000-0002-3780-8041
author2_role author
dc.subject.none.fl_str_mv Circadian rhythms
Luciferase
Promoter activity
Gene expression
Arabidopsis thaliana
Bioluminescence
Reporter assays
Microplate luminometer
topic Circadian rhythms
Luciferase
Promoter activity
Gene expression
Arabidopsis thaliana
Bioluminescence
Reporter assays
Microplate luminometer
description Our understanding of the circadian clock function in plants has been markedly assisted by studies with the model species Arabidopsis thaliana. Molecular and genetics approaches have delivered a comprehensive view of the transcriptional regulatory networks underlying the Arabidopsis circadian system. The use of the luciferase as a reporter allowed the precise in vivo determination of circadian periods, phases, and amplitudes of clock promoter activities with unprecedented temporal resolution. An increasing repertoire of fine-tuned luciferases together with additional applications such as translational fusions or bioluminescence molecular complementation assays have considerably expanded our view of circadian protein expression and activity, far beyond transcriptional regulation. Further applications have focused on the in vivo simultaneous examination of rhythms in different parts of the plant. The use of intact versus excised plant organs has also provided a glimpse on both the organ-specific and autonomy of the clocks and the importance of long distance communication for circadian function. This chapter provides a basic protocol for in vivo high-throughput monitoring of circadian rhythms in Arabidopsis seedlings using bioluminescent reporters and a microplate luminometer.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Capítol de llibre
http://purl.org/coar/resource_type/c_3248
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/259303
https://dx.doi.org/urn:doi:10.1007/978-1-0716-2249-0_27
url https://ddd.uab.cat/record/259303
https://dx.doi.org/urn:doi:10.1007/978-1-0716-2249-0_27
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-106653GB-I00
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CEX2019-000902-S
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana
publisher.none.fl_str_mv Humana
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
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