Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd

Bottle gourd, a common vegetable in the human diet, has been valued for its medicinal and energetic properties. In this experiment, the time-resolved analysis of the changes in the proteins’ electrophoretic patterning of the seed development at different crossing periods was studied in bottle gourd...

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Authors: Malik, Anurag, Singh Mor, Virender, Punia, Himani, Duhan, D. S., Tokas, Jayanti, Bhuker, Axay, Nasser Alyemeni, Mohammed, Shakoor, Awais
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Universitat de Lleida (UdL)
Repository:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/463089
Online Access:https://doi.org/10.3390/cimb45020088
https://hdl.handle.net/10459.1/463089
Access Level:Open access
Keyword:Crossing periods
Label-free quantitation
Plant proteomics
Seed vigor
SDS-PAGE
Llavors
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spelling Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourdMalik, AnuragSingh Mor, VirenderPunia, HimaniDuhan, D. S.Tokas, JayantiBhuker, AxayNasser Alyemeni, MohammedShakoor, AwaisCrossing periodsLabel-free quantitationPlant proteomicsSeed vigorSDS-PAGELlavorsBottle gourd, a common vegetable in the human diet, has been valued for its medicinal and energetic properties. In this experiment, the time-resolved analysis of the changes in the proteins’ electrophoretic patterning of the seed development at different crossing periods was studied in bottle gourd using label-free quantitative proteomics. Hybrid HBGH-35 had the highest observed protein levels at the 4th week of the crossing period (F4) compared to the parental lines, viz. G-2 (M) and Pusa Naveen (F). The crossing period is significantly correlated with grain filling and reserve accumulation. The observed protein expression profile after storage was related to seed maturation and grain filling in bottle gourds. A total of 2517 proteins were identified in differentially treated bottle gourd fruits, and 372 proteins were differentially expressed between different crossing periods. Proteins related to carbohydrate and energy metabolism, anthocyanin biosynthesis, cell stress response, and fruit firmness were characterized and quantified. Some proteins were involved in the development, while others were engaged in desiccation and the early grain-filling stage. F4 was distinguished by an increase in the accumulation of low molecular weight proteins and enzymes such as amylase, a serine protease, and trypsin inhibitors. The seed vigor also followed similar patterns of differential expression of seed storage proteins. Our findings defined a new window during seed production, which showed that at F4, maximum photosynthetic assimilates accumulated, resulting in an enhanced source–sink relationship and improved seed production. Our study attempts to observe the protein expression profiling pattern under different crossing periods using label-free quantitative proteomics in bottle gourd. It will facilitate future detailed investigation of the protein associated with quality traits and the agronomic importance of bottle gourd through selective breeding programs.he authors are thankful to the Department of Vegetable Science, CCS HAU, and Hisar for providing the seeds of bottle gourd parental lines. The authors are also grateful to the Department of Seed Science and Technology for providing all the necessary facilities to research the field trials at the departmental farm and CCS Haryana Agricultural University, Hisar, for providing the required facilities. The authors would also like to extend their sincere appreciation to the Researchers Supporting Project (RSP2023R180) at King Saud University, Riyadh, Saudi Arabia.MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/cimb45020088https://hdl.handle.net/10459.1/463089reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a https://doi.org/10.3390/cimb45020088Current Issues in Molecular Biology, 2023, vol. 45, núm. 2, p. 1-24cc-by (c) Malik et al., 2023Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4630892026-06-24T12:42:17Z
dc.title.none.fl_str_mv Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
title Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
spellingShingle Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
Malik, Anurag
Crossing periods
Label-free quantitation
Plant proteomics
Seed vigor
SDS-PAGE
Llavors
title_short Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
title_full Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
title_fullStr Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
title_full_unstemmed Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
title_sort Development and optimization of label-free quantitative proteomics under different crossing periods of bottle gourd
dc.creator.none.fl_str_mv Malik, Anurag
Singh Mor, Virender
Punia, Himani
Duhan, D. S.
Tokas, Jayanti
Bhuker, Axay
Nasser Alyemeni, Mohammed
Shakoor, Awais
author Malik, Anurag
author_facet Malik, Anurag
Singh Mor, Virender
Punia, Himani
Duhan, D. S.
Tokas, Jayanti
Bhuker, Axay
Nasser Alyemeni, Mohammed
Shakoor, Awais
author_role author
author2 Singh Mor, Virender
Punia, Himani
Duhan, D. S.
Tokas, Jayanti
Bhuker, Axay
Nasser Alyemeni, Mohammed
Shakoor, Awais
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Crossing periods
Label-free quantitation
Plant proteomics
Seed vigor
SDS-PAGE
Llavors
topic Crossing periods
Label-free quantitation
Plant proteomics
Seed vigor
SDS-PAGE
Llavors
description Bottle gourd, a common vegetable in the human diet, has been valued for its medicinal and energetic properties. In this experiment, the time-resolved analysis of the changes in the proteins’ electrophoretic patterning of the seed development at different crossing periods was studied in bottle gourd using label-free quantitative proteomics. Hybrid HBGH-35 had the highest observed protein levels at the 4th week of the crossing period (F4) compared to the parental lines, viz. G-2 (M) and Pusa Naveen (F). The crossing period is significantly correlated with grain filling and reserve accumulation. The observed protein expression profile after storage was related to seed maturation and grain filling in bottle gourds. A total of 2517 proteins were identified in differentially treated bottle gourd fruits, and 372 proteins were differentially expressed between different crossing periods. Proteins related to carbohydrate and energy metabolism, anthocyanin biosynthesis, cell stress response, and fruit firmness were characterized and quantified. Some proteins were involved in the development, while others were engaged in desiccation and the early grain-filling stage. F4 was distinguished by an increase in the accumulation of low molecular weight proteins and enzymes such as amylase, a serine protease, and trypsin inhibitors. The seed vigor also followed similar patterns of differential expression of seed storage proteins. Our findings defined a new window during seed production, which showed that at F4, maximum photosynthetic assimilates accumulated, resulting in an enhanced source–sink relationship and improved seed production. Our study attempts to observe the protein expression profiling pattern under different crossing periods using label-free quantitative proteomics in bottle gourd. It will facilitate future detailed investigation of the protein associated with quality traits and the agronomic importance of bottle gourd through selective breeding programs.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/cimb45020088
https://hdl.handle.net/10459.1/463089
url https://doi.org/10.3390/cimb45020088
https://hdl.handle.net/10459.1/463089
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.3390/cimb45020088
Current Issues in Molecular Biology, 2023, vol. 45, núm. 2, p. 1-24
dc.rights.none.fl_str_mv cc-by (c) Malik et al., 2023
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Malik et al., 2023
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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