Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain

A remarkable percentage of acute gastroenteritis cases remain etiologically undiagnosed. The aim of the study was to determine the prevalence of common and emerging enteric viruses, such as novel human astroviruses, among undiagnosed samples from children with acute gastroenteritis. Epidemiological...

Descripción completa

Detalles Bibliográficos
Autores: Vu, Diem Lan, Sabrià Sunyé, Aurora, Aregall, Núria, Michl, Kristina, Rodríguez Garrido, Virginia, Goterris, Lidia, Bosch, Albert, Pintó Solé, Rosa María, Guix Arnau, Susana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176294
Acceso en línea:https://hdl.handle.net/2445/176294
Access Level:acceso abierto
Palabra clave:Gastroenteritis
Infants malalts
Adenovirus
Epidemiologia
Sick children
Adenoviruses
Epidemiology
id ES_531202df3cd43e4e8f221c37a41d8b38
oai_identifier_str oai:recercat.cat:2445/176294
network_acronym_str ES
network_name_str España
repository_id_str
spelling Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in SpainVu, Diem LanSabrià Sunyé, AuroraAregall, NúriaMichl, KristinaRodríguez Garrido, VirginiaGoterris, LidiaBosch, AlbertPintó Solé, Rosa MaríaGuix Arnau, SusanaGastroenteritisInfants malaltsAdenovirusEpidemiologiaGastroenteritisSick childrenAdenovirusesEpidemiologyA remarkable percentage of acute gastroenteritis cases remain etiologically undiagnosed. The aim of the study was to determine the prevalence of common and emerging enteric viruses, such as novel human astroviruses, among undiagnosed samples from children with acute gastroenteritis. Epidemiological studies for novel human astroviruses are still scarce. Stool samples collected over two consecutive winter seasons (2016-2017) from children with gastroenteritis in Spain, which were negative for bacteria, rotavirus, and adenovirus by routine diagnostics were screened by real-time RT-PCR assays for the presence of classical and novel astrovirus, rotavirus, norovirus GI and GII, sapovirus, and adenovirus. Overall, 220/384 stool samples (57.3%) were positive for at least one virus. Co-infections were identified in 21% of cases. Among a total of 315 viruses identified, adenovirus was the most prevalent (n = 103), followed by rotavirus (n = 51), sapovirus (n = 50), classical astrovirus (n = 43), novel astroviruses (n = 42), and norovirus (n = 26). Novel astroviruses were present in 13.3% of virus-positive cases. Most novel astroviruses were found in children <2-year-old (30/39 children, 77%, p = 0.01) and were found in co-infection (66%). Only classical astroviruses demonstrated significant differences in the Cq values during mono-infections compared to co-infections. In conclusion, common enteric viruses may be frequently found in children with undiagnosed gastroenteritis, indicating the need to implement more sensitive diagnostic methods. Novel astroviruses circulate in the community and could be the cause of gastroenteritis among young children.MDPI2021202120192021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/176294Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/v11070585Viruses, 2019, vol. 11, num. 7https://doi.org/10.3390/v11070585cc-by (c) Vu, Diem Lan et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1762942026-05-29T05:05:01Z
dc.title.none.fl_str_mv Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
title Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
spellingShingle Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
Vu, Diem Lan
Gastroenteritis
Infants malalts
Adenovirus
Epidemiologia
Gastroenteritis
Sick children
Adenoviruses
Epidemiology
title_short Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
title_full Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
title_fullStr Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
title_full_unstemmed Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
title_sort Novel Human Astroviruses: Prevalence and Association with Common Enteric Viruses in Undiagnosed Gastroenteritis Cases in Spain
dc.creator.none.fl_str_mv Vu, Diem Lan
Sabrià Sunyé, Aurora
Aregall, Núria
Michl, Kristina
Rodríguez Garrido, Virginia
Goterris, Lidia
Bosch, Albert
Pintó Solé, Rosa María
Guix Arnau, Susana
author Vu, Diem Lan
author_facet Vu, Diem Lan
Sabrià Sunyé, Aurora
Aregall, Núria
Michl, Kristina
Rodríguez Garrido, Virginia
Goterris, Lidia
Bosch, Albert
Pintó Solé, Rosa María
Guix Arnau, Susana
author_role author
author2 Sabrià Sunyé, Aurora
Aregall, Núria
Michl, Kristina
Rodríguez Garrido, Virginia
Goterris, Lidia
Bosch, Albert
Pintó Solé, Rosa María
Guix Arnau, Susana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gastroenteritis
Infants malalts
Adenovirus
Epidemiologia
Gastroenteritis
Sick children
Adenoviruses
Epidemiology
topic Gastroenteritis
Infants malalts
Adenovirus
Epidemiologia
Gastroenteritis
Sick children
Adenoviruses
Epidemiology
description A remarkable percentage of acute gastroenteritis cases remain etiologically undiagnosed. The aim of the study was to determine the prevalence of common and emerging enteric viruses, such as novel human astroviruses, among undiagnosed samples from children with acute gastroenteritis. Epidemiological studies for novel human astroviruses are still scarce. Stool samples collected over two consecutive winter seasons (2016-2017) from children with gastroenteritis in Spain, which were negative for bacteria, rotavirus, and adenovirus by routine diagnostics were screened by real-time RT-PCR assays for the presence of classical and novel astrovirus, rotavirus, norovirus GI and GII, sapovirus, and adenovirus. Overall, 220/384 stool samples (57.3%) were positive for at least one virus. Co-infections were identified in 21% of cases. Among a total of 315 viruses identified, adenovirus was the most prevalent (n = 103), followed by rotavirus (n = 51), sapovirus (n = 50), classical astrovirus (n = 43), novel astroviruses (n = 42), and norovirus (n = 26). Novel astroviruses were present in 13.3% of virus-positive cases. Most novel astroviruses were found in children <2-year-old (30/39 children, 77%, p = 0.01) and were found in co-infection (66%). Only classical astroviruses demonstrated significant differences in the Cq values during mono-infections compared to co-infections. In conclusion, common enteric viruses may be frequently found in children with undiagnosed gastroenteritis, indicating the need to implement more sensitive diagnostic methods. Novel astroviruses circulate in the community and could be the cause of gastroenteritis among young children.
publishDate 2019
dc.date.none.fl_str_mv 2019
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176294
url https://hdl.handle.net/2445/176294
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/v11070585
Viruses, 2019, vol. 11, num. 7
https://doi.org/10.3390/v11070585
dc.rights.none.fl_str_mv cc-by (c) Vu, Diem Lan et al., 2019
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Vu, Diem Lan et al., 2019
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408092011102208
score 15,812429

Similares en LA Referencia