The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles

The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down <i>CHANEL</i> (<i>PhCHL</i>), the <i>ZEITLUPE</...

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Detalles Bibliográficos
Autores: Terry, Marta I., Pérez-Sanz, Fernando, Díaz-Galián, M. Victoria, Pérez de los Cobos, Felipe, Navarro, Pedro J., Egea-Cortines, Marcos, Weiss, Julia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/180706
Acceso en línea:http://hdl.handle.net/10261/180706
Access Level:acceso abierto
Palabra clave:Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
image analysis
Hairpin RNA
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spelling The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent ProfilesTerry, Marta I.Pérez-Sanz, FernandoDíaz-Galián, M. VictoriaPérez de los Cobos, FelipeNavarro, Pedro J.Egea-Cortines, MarcosWeiss, JuliaFloral scentPetal developmentGrowth ratePhenomicsCircadian clockZEITLUPEimage analysisHairpin RNAThe floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down <i>CHANEL</i> (<i>PhCHL</i>), the <i>ZEITLUPE</i> ortholog in petunia (<i>PhCHL</i>) by hairpin RNAs. Plants with low <i>PhCHL</i> mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by <i>RNAi:PhCHL</i>, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of <i>PhCHL</i>. In contrast, petal growth displayed lower growth rate in <i>RNAi:PhCHL</i>. Decreased levels of <i>PhCHL</i> caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of <i>PhCHL</i> in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of <i>PhCHL</i> on organ development indicate an organ-specific interpretation of the down regulation of <i>PhCHL</i>. Through the control of both timing and quantitative volatile emissions, <i>PhCHL</i> appears to be a major coordinator of scent profiles.This research was funded by Fundación Seneca 19398/PI/14, 19895/GERM/15 and MC BFU-2017 88300-C2-1-R and BFU-2017 88300-C2-2-RWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI)Multidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas (España)Fundación SénecaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/180706reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/cells8040343Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1807062026-05-22T06:33:51Z
dc.title.none.fl_str_mv The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
spellingShingle The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
Terry, Marta I.
Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
image analysis
Hairpin RNA
title_short The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_full The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_fullStr The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_full_unstemmed The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_sort The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
dc.creator.none.fl_str_mv Terry, Marta I.
Pérez-Sanz, Fernando
Díaz-Galián, M. Victoria
Pérez de los Cobos, Felipe
Navarro, Pedro J.
Egea-Cortines, Marcos
Weiss, Julia
author Terry, Marta I.
author_facet Terry, Marta I.
Pérez-Sanz, Fernando
Díaz-Galián, M. Victoria
Pérez de los Cobos, Felipe
Navarro, Pedro J.
Egea-Cortines, Marcos
Weiss, Julia
author_role author
author2 Pérez-Sanz, Fernando
Díaz-Galián, M. Victoria
Pérez de los Cobos, Felipe
Navarro, Pedro J.
Egea-Cortines, Marcos
Weiss, Julia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Fundación Séneca
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
image analysis
Hairpin RNA
topic Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
image analysis
Hairpin RNA
description The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down <i>CHANEL</i> (<i>PhCHL</i>), the <i>ZEITLUPE</i> ortholog in petunia (<i>PhCHL</i>) by hairpin RNAs. Plants with low <i>PhCHL</i> mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by <i>RNAi:PhCHL</i>, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of <i>PhCHL</i>. In contrast, petal growth displayed lower growth rate in <i>RNAi:PhCHL</i>. Decreased levels of <i>PhCHL</i> caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of <i>PhCHL</i> in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of <i>PhCHL</i> on organ development indicate an organ-specific interpretation of the down regulation of <i>PhCHL</i>. Through the control of both timing and quantitative volatile emissions, <i>PhCHL</i> appears to be a major coordinator of scent profiles.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/180706
url http://hdl.handle.net/10261/180706
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/cells8040343
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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