The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes

The definitive version is available at: http://www3.interscience.wiley.com/journal/118538954/home

Detalles Bibliográficos
Autores: Ramos Escribano, Javier, Matamoros Galindo, Manuel Ángel, Naya, Loreto, James, Euan Kevin, Rouhier, Nicolas, Sato, Shusei, Tabata, Satoshi, Becana Ausejo, Manuel
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/12184
Acceso en línea:http://hdl.handle.net/10261/12184
Access Level:acceso abierto
Palabra clave:glutathione peroxidases
Metal toxicity
model legumes
nitric oxide
plastids
salt stress
starch
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spelling The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumesRamos Escribano, JavierMatamoros Galindo, Manuel ÁngelNaya, LoretoJames, Euan KevinRouhier, NicolasSato, ShuseiTabata, SatoshiBecana Ausejo, Manuelglutathione peroxidasesMetal toxicitymodel legumesnitric oxideplastidssalt stressstarchThe definitive version is available at: http://www3.interscience.wiley.com/journal/118538954/home• Despite the multiple roles played by antioxidants in rhizobia–legume symbioses, little is known about glutathione peroxidases (GPXs) in legumes. Here the characterization of six GPX genes of Lotus japonicus is reported. • Expression of GPX genes was analysed by quantitative reverse transcription–polymerase chain reaction in L. japonicus and Lotus corniculatus plants exposed to various treatments known to generate reactive oxygen and/or nitrogen species. • LjGPX1 and LjGPX3 were the most abundantly expressed genes in leaves, roots and nodules. Compared with roots, LjGPX1 and LjGPX6 were highly expressed in leaves and LjGPX3 and LjGPX6 in nodules. In roots, salinity decreased GPX4 expression, aluminium decreased expression of the six genes, and cadmium caused up-regulation of GPX3, GPX4 and GPX5 after 1 h and down-regulation of GPX1, GPX2, GPX4 and GPX6 after 3–24 h. Exposure of roots to sodium nitroprusside (a nitric oxide donor) for 1 h increased the mRNA levels of GPX4 and GPX6 by 3.3- and 30-fold, respectively. Thereafter, the GPX6 mRNA level remained consistently higher than that of the control. Immunogold labelling revealed the presence of GPX proteins in root and nodule amyloplasts and in leaf chloroplasts of L. japonicus and other legumes. Labelling was associated with starch grains. • These results underscore the differential regulation of GPX expression in response to cadmium, aluminium and nitric oxide, and strongly support a role for GPX6 and possibly other GPX genes in stress and/or metabolic signalling.This work was supported by grants from the European Commission (FP6-2003-INCO-DEV2-517617), Spanish Ministry of Education and Science (AGL2005-01404) and Gobierno de Aragón (group A53).Peer reviewedJohn Wiley & Sons200920092009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_650186269 bytesapplication/pdfhttp://hdl.handle.net/10261/12184reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www3.interscience.wiley.com/cgi-bin/fulltext/121426049/PDFSTARTinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/121842026-05-22T06:33:51Z
dc.title.none.fl_str_mv The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
title The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
spellingShingle The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
Ramos Escribano, Javier
glutathione peroxidases
Metal toxicity
model legumes
nitric oxide
plastids
salt stress
starch
title_short The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
title_full The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
title_fullStr The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
title_full_unstemmed The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
title_sort The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes
dc.creator.none.fl_str_mv Ramos Escribano, Javier
Matamoros Galindo, Manuel Ángel
Naya, Loreto
James, Euan Kevin
Rouhier, Nicolas
Sato, Shusei
Tabata, Satoshi
Becana Ausejo, Manuel
author Ramos Escribano, Javier
author_facet Ramos Escribano, Javier
Matamoros Galindo, Manuel Ángel
Naya, Loreto
James, Euan Kevin
Rouhier, Nicolas
Sato, Shusei
Tabata, Satoshi
Becana Ausejo, Manuel
author_role author
author2 Matamoros Galindo, Manuel Ángel
Naya, Loreto
James, Euan Kevin
Rouhier, Nicolas
Sato, Shusei
Tabata, Satoshi
Becana Ausejo, Manuel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv glutathione peroxidases
Metal toxicity
model legumes
nitric oxide
plastids
salt stress
starch
topic glutathione peroxidases
Metal toxicity
model legumes
nitric oxide
plastids
salt stress
starch
description The definitive version is available at: http://www3.interscience.wiley.com/journal/118538954/home
publishDate 2009
dc.date.none.fl_str_mv 2009
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/12184
url http://hdl.handle.net/10261/12184
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www3.interscience.wiley.com/cgi-bin/fulltext/121426049/PDFSTART
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 86269 bytes
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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