Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers

Legionella pneumophila is a significant public health threat, responsible for severe diseases such as Legionnaires’ disease. Traditional detection methods are often labour-intensive, time-consuming, and require sophisticated equipment. This study introduces smart fluorogenic polymeric materials for...

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Autores: Arnáiz Alonso, Ana, Guembe García, Marta, Martínez Yubero, Mario Andrés, Trigo López, Miriam, González, Edurne, Otsuka, Issei, Vallejos Calzada, Saúl
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Burgos (UBU)
Repositorio:Repositorio Institucional de la Universidad de Burgos (RIUBU)
OAI Identifier:oai:riubu.ubu.es:10259/9747
Acceso en línea:http://hdl.handle.net/10259/9747
Access Level:acceso abierto
Palabra clave:Polyacrylic films
Linear polymer
Electrospun nanofibers
Fluorescence
Legionella
Smart material
Polymeric sensors
Química orgánica
Polímeros
Chemistry, Organic
Polymers
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spelling Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibersArnáiz Alonso, AnaGuembe García, MartaMartínez Yubero, Mario AndrésTrigo López, MiriamGonzález, EdurneOtsuka, IsseiVallejos Calzada, SaúlPolyacrylic filmsLinear polymerElectrospun nanofibersFluorescenceLegionellaSmart materialPolymeric sensorsQuímica orgánicaPolímerosChemistry, OrganicPolymersLegionella pneumophila is a significant public health threat, responsible for severe diseases such as Legionnaires’ disease. Traditional detection methods are often labour-intensive, time-consuming, and require sophisticated equipment. This study introduces smart fluorogenic polymeric materials for the naked-eye detection of L. pneumophila via protease activity. These materials, prepared as hydrophilic films, cellulose-coated linear copolymers, and electrospun nanofibers, operate on an OFF/ON FRET system, emitting fluorescence under UV light upon interaction with L. pneumophila proteases. Characterisation confirmed the successful immobilisation of the peptide substrate and its response to proteases. The sensors showed moderate to high sensitivity and specificity, with detection limits of 2.91 × 105, 3.64 × 10⁵, and 4.04 × 105 CFUs/mL for the film, copolymer, and nanofiber formats, respectively. Cross-reactivity tests identified only Pseudomonas aeruginosa as an interferent. This novel approach offers rapid, simple, and cost-effective L. pneumophila detection with visible results under UV light, suitable for clinical and environmental samples. It highlights the potential for broader pathogen detection applications.Agradecemos el apoyo financiero brindado por todos los financiadores. Se agradece el apoyo financiero proporcionado por el Fondo Europeo de Desarrollo Regional (FEDER, FEDER) y la Junta de Castilla y León -Consejería de Educación, Junta de Castilla y León - ( BU025P23 ). Este trabajo contó con el apoyo de la Junta de Castilla y León y el Ministerio de Ciencia e Innovación MICIN y el PRTR NextGenerationEU de la Unión Europea . El autor Saúl Vallejos recibió la beca BG22/00086 financiada por el Ministerio de Universidades de España . La autora Marta Guembe-García recibió financiación del Ministerio de Universidades-Unión Europea de España en el marco del RD 289/2021 NextGeneration EU para su puesto de posdoctorado. También agradecemos el apoyo financiero proporcionado por MCIN/AEI/ 10.13039/501100011033 y por “ FEDER Una manera de hacer Europa ” (subvención PID2020-113264RB-I00 ). Edurne González agradece el apoyo financiero del Ministerio de Ciencia e Innovación español (Proyectos de I+D+i, subvención PID2020-117628RJ-I00 ). Issei Otsuka agradece el apoyo financiero del Centro Nacional Francés de Investigación Científica (subvención Emergence International).Elsevier202420242025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfvideo/mp4http://hdl.handle.net/10259/9747reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)instname:Universidad de Burgos (UBU)InglésSensors and Actuators B: Chemical. 2025, V. 425, 136976https://doi.org/10.1016/j.snb.2024.136976info:eu-repo/grantAgreement/Junta de Castilla y León//BU025P23info:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/BG22%2F00086info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117628RJ-I00Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riubu.ubu.es:10259/97472026-05-28T07:56:11Z
dc.title.none.fl_str_mv Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
title Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
spellingShingle Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
Arnáiz Alonso, Ana
Polyacrylic films
Linear polymer
Electrospun nanofibers
Fluorescence
Legionella
Smart material
Polymeric sensors
Química orgánica
Polímeros
Chemistry, Organic
Polymers
title_short Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
title_full Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
title_fullStr Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
title_full_unstemmed Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
title_sort Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
dc.creator.none.fl_str_mv Arnáiz Alonso, Ana
Guembe García, Marta
Martínez Yubero, Mario Andrés
Trigo López, Miriam
González, Edurne
Otsuka, Issei
Vallejos Calzada, Saúl
author Arnáiz Alonso, Ana
author_facet Arnáiz Alonso, Ana
Guembe García, Marta
Martínez Yubero, Mario Andrés
Trigo López, Miriam
González, Edurne
Otsuka, Issei
Vallejos Calzada, Saúl
author_role author
author2 Guembe García, Marta
Martínez Yubero, Mario Andrés
Trigo López, Miriam
González, Edurne
Otsuka, Issei
Vallejos Calzada, Saúl
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Polyacrylic films
Linear polymer
Electrospun nanofibers
Fluorescence
Legionella
Smart material
Polymeric sensors
Química orgánica
Polímeros
Chemistry, Organic
Polymers
topic Polyacrylic films
Linear polymer
Electrospun nanofibers
Fluorescence
Legionella
Smart material
Polymeric sensors
Química orgánica
Polímeros
Chemistry, Organic
Polymers
description Legionella pneumophila is a significant public health threat, responsible for severe diseases such as Legionnaires’ disease. Traditional detection methods are often labour-intensive, time-consuming, and require sophisticated equipment. This study introduces smart fluorogenic polymeric materials for the naked-eye detection of L. pneumophila via protease activity. These materials, prepared as hydrophilic films, cellulose-coated linear copolymers, and electrospun nanofibers, operate on an OFF/ON FRET system, emitting fluorescence under UV light upon interaction with L. pneumophila proteases. Characterisation confirmed the successful immobilisation of the peptide substrate and its response to proteases. The sensors showed moderate to high sensitivity and specificity, with detection limits of 2.91 × 105, 3.64 × 10⁵, and 4.04 × 105 CFUs/mL for the film, copolymer, and nanofiber formats, respectively. Cross-reactivity tests identified only Pseudomonas aeruginosa as an interferent. This novel approach offers rapid, simple, and cost-effective L. pneumophila detection with visible results under UV light, suitable for clinical and environmental samples. It highlights the potential for broader pathogen detection applications.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10259/9747
url http://hdl.handle.net/10259/9747
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sensors and Actuators B: Chemical. 2025, V. 425, 136976
https://doi.org/10.1016/j.snb.2024.136976
info:eu-repo/grantAgreement/Junta de Castilla y León//BU025P23
info:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/BG22%2F00086
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117628RJ-I00
dc.rights.none.fl_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
video/mp4
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)
instname:Universidad de Burgos (UBU)
instname_str Universidad de Burgos (UBU)
reponame_str Repositorio Institucional de la Universidad de Burgos (RIUBU)
collection Repositorio Institucional de la Universidad de Burgos (RIUBU)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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