Rapid outbreak sequencing of Ebola virus in Sierra Leone identifies transmission chains linked to sporadic cases.

Toendthelargest knownoutbreak of Ebola virus disease (EVD) in West Africa and to prevent new transmissions, rapid epidemiological tracing of cases and contacts was required. The ability to quickly identify unknown sources and chains of transmission is key to ending the EVD epidemic and of even great...

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Detalles Bibliográficos
Autores: Arias Esteban, Armando, Watson, Simon J., Asogun, Danny, Tobin, Ekaete Alice, Jia Lu, Phan, My V. T., Jah, Umaru, Guetiya Wadoum, Raoul Emeric, Meredith, Luke, Thorne, Lucy, Caddy, Sarah, Tarawalie, Alimamy, Langat, Pinky, Dudas, Gytis, Faria, Nuno R., Dellicour, Simon, Kamara, Abdul, Kargbo, Brima, Osaio Kamara, Brima, Gevao, Sahr, Cooper, Daniel, Newport, Matthew, Horby, Peter, Dunning, Jake, Sahr, Foday, Brooks, Tim, Simpson, Andrew J.H., Groppelli, Elisabetta, Guoying Liu, Mulakken, Nisha, Rhodes, Kate, Akpablie, James, Yoti, Zabulon, Lamunu, Margaret, Vitto, Esther, Otim, Patrick, Owilli, Collins, Boateng, Isaac, Okoror, Lawrence, Omomoh, Emmanuel, Oyakhilome, Jennifer, Omiunu, Racheal, Yemisis, Ighodalo, Adomeh, Donatus, Ehikhiametalor, Solomon, Akhilomen, Patience, Aire, Chris, Kurth, Andreas, Cook, Nicola, Baumann, Jan, Gabriel, Martin, Wölfel, Roman, Di Caro, Antonino, Carroll, Miles W., Günther, Stephan, Redd, John, Naidoo, Dhamari, Pybus, Oliver G., Rambaut, Andrew, Kellam, Paul, Goodfellow, Ian, Cotten, Matthew
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/46897
Acceso en línea:https://hdl.handle.net/10578/46897
Access Level:acceso abierto
Palabra clave:Ebola virus
Evolution
Outbreak sequencing.
Transmission
Descripción
Sumario:Toendthelargest knownoutbreak of Ebola virus disease (EVD) in West Africa and to prevent new transmissions, rapid epidemiological tracing of cases and contacts was required. The ability to quickly identify unknown sources and chains of transmission is key to ending the EVD epidemic and of even greater importance in the context of recent reports of Ebola virus (EBOV) persistence in survivors. Phylogenetic analysis of complete EBOV genomes can provide important information onthesource of any newinfection. A local deep sequencing facility was established at the Mateneh Ebola Treatment Centre in central Sierra Leone. The facility included all wetlab and computational resources to rapidly process EBOV diagnostic samples into full genome sequences. We produced 554 EBOVgenomesfromEVDcasesacrossSierra Leone.These genomes provided a detailed description of EBOV evolution and facilitated phylogenetic tracking of new EVD cases. Importantly, we showthat linked genomicand epidemiological data can not only support contact tracing but also identify unconventional transmission chains involving body fluids, including semen. Rapid EBOV genome sequencing, when linked to epidemiological information and a comprehensive database of virus sequences across the outbreak, provided a powerful tool for public health epidemic control efforts.

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