Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida

In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple...

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Autores: Huertas-Rosales, Óscar, Ramos-González, María Isabel, Espinosa-Urgel, Manuel
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/375552
Acceso en línea:http://hdl.handle.net/10261/375552
https://api.elsevier.com/content/abstract/scopus_id/84987925401
Access Level:acceso abierto
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spelling Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putidaHuertas-Rosales, ÓscarRamos-González, María IsabelEspinosa-Urgel, ManuelIn the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple-null mutants and by overexpressing each protein (RsmA, RsmE, and RsmI) in different genetic backgrounds. The rsm triple mutant shows reduced swimming and swarming motilities and increased biofilm formation, whereas overexpression of RsmE or RsmI results in reduced bacterial attachment. However, biofilms formed on glass surfaces by the triple mutant are more labile than those of the wild-type strain and are easily detached from the surface, a phenomenon that is not observed on plastic surfaces. Analysis of the expression of adhesins and exopolysaccharides in the different genetic backgrounds suggests that the biofilm phenotypes are due to alterations in the composition of the extracellular matrix and in the timing of synthesis of its elements. We have also studied the expression patterns of Rsm proteins and obtained data that indicate the existence of autoregulation mechanisms.Peer reviewedAmerican Society for MicrobiologyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/375552https://api.elsevier.com/content/abstract/scopus_id/84987925401reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésApplied and environmental microbiologyhttps://journals.asm.org/doi/10.1128/aem.01724-16Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3755522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
title Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
spellingShingle Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
Huertas-Rosales, Óscar
title_short Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
title_full Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
title_fullStr Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
title_full_unstemmed Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
title_sort Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida
dc.creator.none.fl_str_mv Huertas-Rosales, Óscar
Ramos-González, María Isabel
Espinosa-Urgel, Manuel
author Huertas-Rosales, Óscar
author_facet Huertas-Rosales, Óscar
Ramos-González, María Isabel
Espinosa-Urgel, Manuel
author_role author
author2 Ramos-González, María Isabel
Espinosa-Urgel, Manuel
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple-null mutants and by overexpressing each protein (RsmA, RsmE, and RsmI) in different genetic backgrounds. The rsm triple mutant shows reduced swimming and swarming motilities and increased biofilm formation, whereas overexpression of RsmE or RsmI results in reduced bacterial attachment. However, biofilms formed on glass surfaces by the triple mutant are more labile than those of the wild-type strain and are easily detached from the surface, a phenomenon that is not observed on plastic surfaces. Analysis of the expression of adhesins and exopolysaccharides in the different genetic backgrounds suggests that the biofilm phenotypes are due to alterations in the composition of the extracellular matrix and in the timing of synthesis of its elements. We have also studied the expression patterns of Rsm proteins and obtained data that indicate the existence of autoregulation mechanisms.
publishDate 2016
dc.date.none.fl_str_mv 2016
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/375552
https://api.elsevier.com/content/abstract/scopus_id/84987925401
url http://hdl.handle.net/10261/375552
https://api.elsevier.com/content/abstract/scopus_id/84987925401
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Applied and environmental microbiology
https://journals.asm.org/doi/10.1128/aem.01724-16
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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