Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions

12 Pág.

Detalles Bibliográficos
Autores: Moreno-Pérez, Manuel G., Bera, Sayanta, McLeish, Michael, Fraile, Aurora, García-Arenal, F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/296187
Acceso en línea:http://hdl.handle.net/10261/296187
https://api.elsevier.com/content/abstract/scopus_id/85142741450
Access Level:acceso abierto
Palabra clave:Across-host trade-offs
Costs of virulence
Necrotic local lesions
Pepper
Tobamovirus
Virus diversity
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spelling Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesionsMoreno-Pérez, Manuel G.Bera, SayantaMcLeish, MichaelFraile, AuroraGarcía-Arenal, F.Across-host trade-offsCosts of virulenceNecrotic local lesionsPepperTobamovirusVirus diversity12 Pág.An instance of host range evolution relevant to plant virus disease control is resistance breaking. Resistance breaking can be hindered by across-host fitness trade-offs generated by negative effects of resistance-breaking mutations on the virus fitness in susceptible hosts. Different mutations in pepper mild mottle virus (PMMoV) coat protein result in the breaking in pepper plants of the resistance determined by the L3 resistance allele. Of these, mutation M138N is widespread in PMMoV populations, despite associated fitness penalties in within-host multiplication and survival. The stability of mutation M138N was analysed by serial passaging in L3 resistant plants. Appearance on passaging of necrotic local lesions (NLL), indicating an effective L3 resistance, showed reversion to nonresistance-breaking phenotypes was common. Most revertant genotypes had the mutation N138K, which affects the properties of the virus particle, introducing a penalty of reversion. Hence, the costs of reversion may determine the evolution of resistance-breaking in addition to resistance-breaking costs. The genetic diversity of the virus population in NLL was much higher than in systemically infected tissues, and included mutations reported to break L3 resistance other than M138N. Infectivity assays on pepper genotypes with different L alleles showed high phenotypic diversity in respect to L alleles in NLL, including phenotypes not reported in nature. Thus, high diversity at NLL may potentiate the appearance of genotypes that enable the colonization of new host genotypes or species. Collectively, the results of this study contribute to better understanding the evolutionary dynamics of resistance breaking and host-range expansions.This work was funded in part by Plan Estatal de I+D+i, Ministerio de Ciencia, Innovación y Universidades, Spain (grant RTI2018-094302- B- I00 to F.G-A.). S.B. was supported by a scholarship of an Erasmus Mundus EU programme (BRAVE, agreement 2013-2536/001-001). Miguel Ángel Mora and Antolín López Quirós provided excellent technical support. We thank Dr Tomás Canto for providing access to electron microscope facilities at Margarita Salas Center for Biological Research, CSIC, Madrid, and Rafael Núñez Ramírez for assistance with electron microscopy.Peer reviewedWiley-VCHMinisterio de Ciencia, Innovación y Universidades (España)European CommissionMcLeish, Michael [0000-0002-4525-5221]Fraile, Aurora [0000-0002-2785-7931]García-Arenal, F. [0000-0002-5327-3200]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/296187https://api.elsevier.com/content/abstract/scopus_id/85142741450reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094302-B-I00info:eu-repo/grantAgreement/EC//2013-2536Centro de Biotecnología y Genómica de Plantas (CBGP)https://dx.doi.org/10.1111/mpp.13281Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2961872026-05-22T06:33:51Z
dc.title.none.fl_str_mv Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
title Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
spellingShingle Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
Moreno-Pérez, Manuel G.
Across-host trade-offs
Costs of virulence
Necrotic local lesions
Pepper
Tobamovirus
Virus diversity
title_short Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
title_full Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
title_fullStr Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
title_full_unstemmed Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
title_sort Reversion of a resistance-breaking mutation shows reversion costs and high virus diversity at necrotic local lesions
dc.creator.none.fl_str_mv Moreno-Pérez, Manuel G.
Bera, Sayanta
McLeish, Michael
Fraile, Aurora
García-Arenal, F.
author Moreno-Pérez, Manuel G.
author_facet Moreno-Pérez, Manuel G.
Bera, Sayanta
McLeish, Michael
Fraile, Aurora
García-Arenal, F.
author_role author
author2 Bera, Sayanta
McLeish, Michael
Fraile, Aurora
García-Arenal, F.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
McLeish, Michael [0000-0002-4525-5221]
Fraile, Aurora [0000-0002-2785-7931]
García-Arenal, F. [0000-0002-5327-3200]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Across-host trade-offs
Costs of virulence
Necrotic local lesions
Pepper
Tobamovirus
Virus diversity
topic Across-host trade-offs
Costs of virulence
Necrotic local lesions
Pepper
Tobamovirus
Virus diversity
description 12 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/296187
https://api.elsevier.com/content/abstract/scopus_id/85142741450
url http://hdl.handle.net/10261/296187
https://api.elsevier.com/content/abstract/scopus_id/85142741450
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094302-B-I00
info:eu-repo/grantAgreement/EC//2013-2536
Centro de Biotecnología y Genómica de Plantas (CBGP)
https://dx.doi.org/10.1111/mpp.13281
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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