Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content

A hydrogel formed with phospholipids and fatty acids would be of great interest in the medical field due to the biological relevance that these molecules have in living organisms. However, the tendency of phospholipid mixtures to form vesicular or micellar aggregates at high water content hinders th...

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Autores: Talló, Kirian, Bosch, Manel, Pons Pons, Ramon, Cocera Núñez, Mercedes, López Serrano, Olga
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/195071
Acceso en línea:http://hdl.handle.net/10261/195071
Access Level:acceso abierto
Palabra clave:Supramolecular hydrogel
Highly hydrated hydrogel
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spelling Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water contentTalló, KirianBosch, ManelPons Pons, RamonCocera Núñez, MercedesLópez Serrano, OlgaSupramolecular hydrogelHighly hydrated hydrogelA hydrogel formed with phospholipids and fatty acids would be of great interest in the medical field due to the biological relevance that these molecules have in living organisms. However, the tendency of phospholipid mixtures to form vesicular or micellar aggregates at high water content hinders the formation of this type of hydrogels. In this study, a highly hydrated hydrogel (95% water) was formed with hydrogenated phosphatidylcholine and oleic acid. The preparation method involved a freeze-heating cycle of the aqueous lipid mixture, favouring the supramolecular aggregation of these molecules into a microscopic spongey morphology. Confocal fluorescence imaging showed that the microstructure of the hydrogel is made from the aggregation of giant multilamellar vesicles (5-20 µm diameter) while transmission electron microscopy revealed the existence of nanosized unilamellar vesicles (150 nm diameter) coexisting with lipid lamellae. Despite this type of aggregation, X-ray scattering experiments performed on the hydrogel show almost no correlation between lipid membranes. In terms of rheological properties, the material shows a prevalent elastic behaviour and a low structural strength consequence of non-covalent interactions. With such properties and composition, this structured but easily deformable material might become a useful tool for biomedical applications.The authors would like to acknowledge Jaume Caelles from the SAXS-WAXS service at IQAC for X-ray measurements and Lidia Delgado from CCITUB for cryo-TEM sample preparation. Rheology assays were performed by Susana Vílchez at the Nanostructured Liquid Characterization Unit of CSIC and CIBER-BBN. Authors are also grateful to Lipoid GmbH for kindly providing Phospholipon® 90H and to MINECO for funding this work with the RTC-2016-4957-1 project.We acknowledge support of the publication fee by the CSIC Open Access Support Initiative through its Unit of Information Resources for Research (URICI)Peer reviewedRoyal Society of Chemistry (UK)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Pons Pons, Ramón [0000-0003-4273-9084]López, Olga [0000-0002-3320-5964]Talló, Kirian [0000-0002-9492-1411]Bosch, Manel [0000-0001-5870-6346]Cocera Núñez, Mercedes [0000-0003-1077-052X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/195071reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2016-4957-1http://doi.org/10.1039/C9TB01599JSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1950712026-05-22T06:33:51Z
dc.title.none.fl_str_mv Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
spellingShingle Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
Talló, Kirian
Supramolecular hydrogel
Highly hydrated hydrogel
title_short Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_full Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_fullStr Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_full_unstemmed Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_sort Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
dc.creator.none.fl_str_mv Talló, Kirian
Bosch, Manel
Pons Pons, Ramon
Cocera Núñez, Mercedes
López Serrano, Olga
author Talló, Kirian
author_facet Talló, Kirian
Bosch, Manel
Pons Pons, Ramon
Cocera Núñez, Mercedes
López Serrano, Olga
author_role author
author2 Bosch, Manel
Pons Pons, Ramon
Cocera Núñez, Mercedes
López Serrano, Olga
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Pons Pons, Ramón [0000-0003-4273-9084]
López, Olga [0000-0002-3320-5964]
Talló, Kirian [0000-0002-9492-1411]
Bosch, Manel [0000-0001-5870-6346]
Cocera Núñez, Mercedes [0000-0003-1077-052X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Supramolecular hydrogel
Highly hydrated hydrogel
topic Supramolecular hydrogel
Highly hydrated hydrogel
description A hydrogel formed with phospholipids and fatty acids would be of great interest in the medical field due to the biological relevance that these molecules have in living organisms. However, the tendency of phospholipid mixtures to form vesicular or micellar aggregates at high water content hinders the formation of this type of hydrogels. In this study, a highly hydrated hydrogel (95% water) was formed with hydrogenated phosphatidylcholine and oleic acid. The preparation method involved a freeze-heating cycle of the aqueous lipid mixture, favouring the supramolecular aggregation of these molecules into a microscopic spongey morphology. Confocal fluorescence imaging showed that the microstructure of the hydrogel is made from the aggregation of giant multilamellar vesicles (5-20 µm diameter) while transmission electron microscopy revealed the existence of nanosized unilamellar vesicles (150 nm diameter) coexisting with lipid lamellae. Despite this type of aggregation, X-ray scattering experiments performed on the hydrogel show almost no correlation between lipid membranes. In terms of rheological properties, the material shows a prevalent elastic behaviour and a low structural strength consequence of non-covalent interactions. With such properties and composition, this structured but easily deformable material might become a useful tool for biomedical applications.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/195071
url http://hdl.handle.net/10261/195071
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2016-4957-1
http://doi.org/10.1039/C9TB01599J
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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