An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol

Reduced graphene oxide–carboxymethylcellulose hybrid (CMC–rGO) was used for the development of a novel electrochemical immunosensor for the determination of adiponectin (APN) cytokine. The hybrid material was synthesized by covalent binding of oxidized CMC to GO layers followed by chemical reduction...

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Detalles Bibliográficos
Autores: Arenas Sevillano, Cristian Brenabé, Sánchez Tirado, Esther, Ojeda Fernández, Irene, Gomez-Suarez, C.A., González Cortés, Araceli, Villalonga Santana, Reynaldo, Yáñez-Sedeño Orive, Paloma, Pingarrón Carrazón, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/124311
Acceso en línea:https://hdl.handle.net/20.500.14352/124311
Access Level:acceso abierto
Palabra clave:543
Graphene
Adiponectin
Electrochemical immunosensor
Carboxymethyl cellulose
Mix&Go
Human serum
Química
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/124311
network_acronym_str ES
network_name_str España
repository_id_str
spelling An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffolArenas Sevillano, Cristian BrenabéSánchez Tirado, EstherOjeda Fernández, IreneGomez-Suarez, C.A.González Cortés, AraceliVillalonga Santana, ReynaldoYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuel543GrapheneAdiponectinElectrochemical immunosensorCarboxymethyl celluloseMix&GoHuman serumQuímica2301 Química AnalíticaReduced graphene oxide–carboxymethylcellulose hybrid (CMC–rGO) was used for the development of a novel electrochemical immunosensor for the determination of adiponectin (APN) cytokine. The hybrid material was synthesized by covalent binding of oxidized CMC to GO layers followed by chemical reduction with sodium borohydride. A sandwich-type immunoassay was employed involving the commercial metal-complexes based polymer Mix & Go™ for the stable and oriented immobilization of anti-APN capture antibody. Biotinylated-anti-APN and HRP-Strept were used for the assay configuration. The APN quantification was performed by amperometry at −200 mV using the hydrogen peroxide/hydroquinone enzyme substrate/mediator system. A sigmoidal calibration plot for APN in the 0.1–50 μg/mL range, with a linear portion in the 0.5–10.0 μg/mL APN concentration range was obtained. The calculated limit of detection (3sb/m) was 61 ng/mL APN. The usefulness of the immunosensor was evaluated by analyzing human serum from hypercholesterolemia or diabetes patients.ElsevierUniversidad Complutense de Madrid20152015-09-1220152015-09-12journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/124311reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1243112026-06-02T12:44:21Z
dc.title.none.fl_str_mv An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
title An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
spellingShingle An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
Arenas Sevillano, Cristian Brenabé
543
Graphene
Adiponectin
Electrochemical immunosensor
Carboxymethyl cellulose
Mix&Go
Human serum
Química
2301 Química Analítica
title_short An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
title_full An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
title_fullStr An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
title_full_unstemmed An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
title_sort An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
dc.creator.none.fl_str_mv Arenas Sevillano, Cristian Brenabé
Sánchez Tirado, Esther
Ojeda Fernández, Irene
Gomez-Suarez, C.A.
González Cortés, Araceli
Villalonga Santana, Reynaldo
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author Arenas Sevillano, Cristian Brenabé
author_facet Arenas Sevillano, Cristian Brenabé
Sánchez Tirado, Esther
Ojeda Fernández, Irene
Gomez-Suarez, C.A.
González Cortés, Araceli
Villalonga Santana, Reynaldo
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author_role author
author2 Sánchez Tirado, Esther
Ojeda Fernández, Irene
Gomez-Suarez, C.A.
González Cortés, Araceli
Villalonga Santana, Reynaldo
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Graphene
Adiponectin
Electrochemical immunosensor
Carboxymethyl cellulose
Mix&Go
Human serum
Química
2301 Química Analítica
topic 543
Graphene
Adiponectin
Electrochemical immunosensor
Carboxymethyl cellulose
Mix&Go
Human serum
Química
2301 Química Analítica
description Reduced graphene oxide–carboxymethylcellulose hybrid (CMC–rGO) was used for the development of a novel electrochemical immunosensor for the determination of adiponectin (APN) cytokine. The hybrid material was synthesized by covalent binding of oxidized CMC to GO layers followed by chemical reduction with sodium borohydride. A sandwich-type immunoassay was employed involving the commercial metal-complexes based polymer Mix & Go™ for the stable and oriented immobilization of anti-APN capture antibody. Biotinylated-anti-APN and HRP-Strept were used for the assay configuration. The APN quantification was performed by amperometry at −200 mV using the hydrogen peroxide/hydroquinone enzyme substrate/mediator system. A sigmoidal calibration plot for APN in the 0.1–50 μg/mL range, with a linear portion in the 0.5–10.0 μg/mL APN concentration range was obtained. The calculated limit of detection (3sb/m) was 61 ng/mL APN. The usefulness of the immunosensor was evaluated by analyzing human serum from hypercholesterolemia or diabetes patients.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-09-12
2015
2015-09-12
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/124311
url https://hdl.handle.net/20.500.14352/124311
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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