Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis
Objectives: To characterize the vectors involved in the dissemination of bla CMY-2 genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007. Methods: Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybrid...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | España |
| Recursos: | Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
| Repositorio: | r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| OAI Identifier: | oai:iibsantpau.fundanetsuite.com:p10501 |
| Acesso em linha: | https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10501 https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960963278&doi=10.1093%2fjac%2fdkr286&partnerID=40&md5=62e3be4a3b566844c60e96ccab389d04 |
| Access Level: | acceso abierto |
| Palavra-chave: | antitoxin bacterial protein beta lactamase AmpC beta lactamase CMY 2 beta lactamase CTX M carbapenemase extended spectrum beta lactamase integrase R391 integrative conjugative elements SXT integrative conjugative element unclassified drug article bacterial gene bacterium isolate genetic organization hybridization nonhuman plasmid polymerase chain reaction Proteus mirabilis Southern blotting Bacterial Proteins beta-Lactamases Conjugation, Genetic DNA, Bacterial Drug Resistance, Multiple, Bacterial Humans Integrases Interspersed Repetitive Sequences Microbial Sensitivity Tests Plasmids Proteus Infections |
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Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilisMata C.Navarro F.Miró E.Walsh T.R.Mirelis B.Toleman M.antitoxinbacterial proteinbeta lactamase AmpCbeta lactamase CMY 2beta lactamase CTX Mcarbapenemaseextended spectrum beta lactamaseintegraseR391 integrative conjugative elementsSXT integrative conjugative elementunclassified drugarticlebacterial genebacterium isolategenetic organizationhybridizationnonhumanplasmidpolymerase chain reactionProteus mirabilisSouthern blottingBacterial Proteinsbeta-LactamasesConjugation, GeneticDNA, BacterialDrug Resistance, Multiple, BacterialHumansIntegrasesInterspersed Repetitive SequencesMicrobial Sensitivity TestsPlasmidsProteus InfectionsProteus mirabilisObjectives: To characterize the vectors involved in the dissemination of bla CMY-2 genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007. Methods: Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybridization with ampC and replicon probes. Isolates that could not be characterized were examined for the presence of SXT/R391-like elements. To demonstrate the involvement of these elements in the dissemination of bla CMY-2, we performed a PCR amplification of the integrase (int) and toxin/antitoxin (TA) genes from SXT/R391-like integrative conjugative elements (ICEs). Later on, I-Ceu-I PFGE gels and hybridization with bla CMY-2, int and prfC probes were performed. The genetic organization of bla CMY-2 was also studied. Results: ampC genes were located on large conjugative plasmids in 11 of the 19 (58%) P. mirabilis studied. However, in eight of these isolates a plasmid was not involved in the mobilization of ampC genes. I-Ceu-I PFGE and hybridization analyses revealed thatbla CMY-2 were chromosomally located in these eight P. mirabilis isolates. The genetic organization of bla CMY-2 and hybridization analyses revealed that bla CMY-2 was carried by an ICE almost identical to ICEPmiJpan1 in seven out of these eight isolates. Conclusions: The prevalence of ICEs carrying bla CMY-2 was surprisingly high [37% (7 out of 19)]. This is the first study giving prevalence data on ICEs carrying bla CMY-2 genes. These results suggest the need to study these mobile genetic elements in the context of dissemination of acquired AmpC ß-lactamases and also of other ß-lactamases, such as extended-spectrum ß-lactamases and carbapenemases. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.OXFORD UNIV PRESS2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10501https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960963278&doi=10.1093%2fjac%2fdkr286&partnerID=40&md5=62e3be4a3b566844c60e96ccab389d04JOURNAL OF ANTIMICROBIAL CHEMOTHERAPYISSN: 03057453ISSNe: 14602091reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p105012026-06-14T12:41:47Z |
| dc.title.none.fl_str_mv |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis |
| title |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis |
| spellingShingle |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis Mata C. antitoxin bacterial protein beta lactamase AmpC beta lactamase CMY 2 beta lactamase CTX M carbapenemase extended spectrum beta lactamase integrase R391 integrative conjugative elements SXT integrative conjugative element unclassified drug article bacterial gene bacterium isolate genetic organization hybridization nonhuman plasmid polymerase chain reaction Proteus mirabilis Southern blotting Bacterial Proteins beta-Lactamases Conjugation, Genetic DNA, Bacterial Drug Resistance, Multiple, Bacterial Humans Integrases Interspersed Repetitive Sequences Microbial Sensitivity Tests Plasmids Proteus Infections Proteus mirabilis |
| title_short |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis |
| title_full |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis |
| title_fullStr |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis |
| title_full_unstemmed |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis |
| title_sort |
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis |
| dc.creator.none.fl_str_mv |
Mata C. Navarro F. Miró E. Walsh T.R. Mirelis B. Toleman M. |
| author |
Mata C. |
| author_facet |
Mata C. Navarro F. Miró E. Walsh T.R. Mirelis B. Toleman M. |
| author_role |
author |
| author2 |
Navarro F. Miró E. Walsh T.R. Mirelis B. Toleman M. |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
antitoxin bacterial protein beta lactamase AmpC beta lactamase CMY 2 beta lactamase CTX M carbapenemase extended spectrum beta lactamase integrase R391 integrative conjugative elements SXT integrative conjugative element unclassified drug article bacterial gene bacterium isolate genetic organization hybridization nonhuman plasmid polymerase chain reaction Proteus mirabilis Southern blotting Bacterial Proteins beta-Lactamases Conjugation, Genetic DNA, Bacterial Drug Resistance, Multiple, Bacterial Humans Integrases Interspersed Repetitive Sequences Microbial Sensitivity Tests Plasmids Proteus Infections Proteus mirabilis |
| topic |
antitoxin bacterial protein beta lactamase AmpC beta lactamase CMY 2 beta lactamase CTX M carbapenemase extended spectrum beta lactamase integrase R391 integrative conjugative elements SXT integrative conjugative element unclassified drug article bacterial gene bacterium isolate genetic organization hybridization nonhuman plasmid polymerase chain reaction Proteus mirabilis Southern blotting Bacterial Proteins beta-Lactamases Conjugation, Genetic DNA, Bacterial Drug Resistance, Multiple, Bacterial Humans Integrases Interspersed Repetitive Sequences Microbial Sensitivity Tests Plasmids Proteus Infections Proteus mirabilis |
| description |
Objectives: To characterize the vectors involved in the dissemination of bla CMY-2 genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007. Methods: Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybridization with ampC and replicon probes. Isolates that could not be characterized were examined for the presence of SXT/R391-like elements. To demonstrate the involvement of these elements in the dissemination of bla CMY-2, we performed a PCR amplification of the integrase (int) and toxin/antitoxin (TA) genes from SXT/R391-like integrative conjugative elements (ICEs). Later on, I-Ceu-I PFGE gels and hybridization with bla CMY-2, int and prfC probes were performed. The genetic organization of bla CMY-2 was also studied. Results: ampC genes were located on large conjugative plasmids in 11 of the 19 (58%) P. mirabilis studied. However, in eight of these isolates a plasmid was not involved in the mobilization of ampC genes. I-Ceu-I PFGE and hybridization analyses revealed thatbla CMY-2 were chromosomally located in these eight P. mirabilis isolates. The genetic organization of bla CMY-2 and hybridization analyses revealed that bla CMY-2 was carried by an ICE almost identical to ICEPmiJpan1 in seven out of these eight isolates. Conclusions: The prevalence of ICEs carrying bla CMY-2 was surprisingly high [37% (7 out of 19)]. This is the first study giving prevalence data on ICEs carrying bla CMY-2 genes. These results suggest the need to study these mobile genetic elements in the context of dissemination of acquired AmpC ß-lactamases and also of other ß-lactamases, such as extended-spectrum ß-lactamases and carbapenemases. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
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https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10501 https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960963278&doi=10.1093%2fjac%2fdkr286&partnerID=40&md5=62e3be4a3b566844c60e96ccab389d04 |
| url |
https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10501 https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960963278&doi=10.1093%2fjac%2fdkr286&partnerID=40&md5=62e3be4a3b566844c60e96ccab389d04 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
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OXFORD UNIV PRESS |
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OXFORD UNIV PRESS |
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JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY ISSN: 03057453 ISSNe: 14602091 reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
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r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
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r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
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