In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins

30 páginas, 3 figuras, 1 resumen gráfico, 3 tablas. -- The definitive version is available at http://www.elsevier.com

Detalles Bibliográficos
Autores: Torre Escudero, Eduardo de la, Pérez Sánchez, Ricardo, Manzano Román, Raúl, Oleaga, Ana
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/85191
Acceso en línea:http://hdl.handle.net/10261/85191
Access Level:acceso abierto
Palabra clave:Schistosoma bovis
Tegument
Proteome
Vascular perfusion
Biotinylation
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spelling In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteinsTorre Escudero, Eduardo de laPérez Sánchez, RicardoManzano Román, RaúlOleaga, AnaSchistosoma bovisTegumentProteomeVascular perfusionBiotinylation30 páginas, 3 figuras, 1 resumen gráfico, 3 tablas. -- The definitive version is available at http://www.elsevier.comSchistosoma bovis is a blood-dwelling fluke of ruminants that lives for years inside the vasculature of their hosts. The parasite tegument covers the surface of the worms and plays a key role in the host–parasite relationship. The parasite molecules expressed at the tegument surface are potential targets for immune or drug intervention. The purpose of this work was the identification of the proteins expressed in vivo on the surface of the tegument of S. bovis adult worms. To accomplish this we used a method based on in vivo vascular perfusion of mice infected with S. bovis which allowed the labelling of the surface of the worms inside the blood vasculature. The biotinylation of parasite inside blood vessels prevents the handling of worms in vitro and hence possible damage to the tegument that could produce results that would be difficult to interpret. Trypsin digestion of biotinylated proteins and subsequent liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) resulted in the identification on the S. bovis tegument of 80 parasite proteins and 28 host proteins. The proteins identified were compared with the findings from other proteomic studies of the schistosome surface. The experimental approach used in this work is a reliable method for selective investigation of the surface of the worms and provides valuable information about the exposed protein repertoire of the tegument of S. bovis in the environmental conditions that the parasite faces inside the blood vessels.This research was funded by project AGL2010-18163 granted by the Spanish Ministry of Science and Innovation.Peer reviewedElsevier2013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/85191reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.jprot.2013.09.020info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/851912026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
title In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
spellingShingle In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
Torre Escudero, Eduardo de la
Schistosoma bovis
Tegument
Proteome
Vascular perfusion
Biotinylation
title_short In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
title_full In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
title_fullStr In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
title_full_unstemmed In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
title_sort In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins
dc.creator.none.fl_str_mv Torre Escudero, Eduardo de la
Pérez Sánchez, Ricardo
Manzano Román, Raúl
Oleaga, Ana
author Torre Escudero, Eduardo de la
author_facet Torre Escudero, Eduardo de la
Pérez Sánchez, Ricardo
Manzano Román, Raúl
Oleaga, Ana
author_role author
author2 Pérez Sánchez, Ricardo
Manzano Román, Raúl
Oleaga, Ana
author2_role author
author
author
dc.subject.none.fl_str_mv Schistosoma bovis
Tegument
Proteome
Vascular perfusion
Biotinylation
topic Schistosoma bovis
Tegument
Proteome
Vascular perfusion
Biotinylation
description 30 páginas, 3 figuras, 1 resumen gráfico, 3 tablas. -- The definitive version is available at http://www.elsevier.com
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/85191
url http://hdl.handle.net/10261/85191
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.jprot.2013.09.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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