Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer

Raffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and qu...

Descripción completa

Detalles Bibliográficos
Autores: Prieto Santiago, Virginia, Cavia Camarero, María del Mar, Barba, Francisco J., Alonso de la Torre, Sara, Carrillo Pérez, Celia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Burgos (UBU)
Repositorio:Repositorio Institucional de la Universidad de Burgos (RIUBU)
OAI Identifier:oai:riubu.ubu.es:10259/6196
Acceso en línea:http://hdl.handle.net/10259/6196
Access Level:acceso abierto
Palabra clave:Triple quadrupole
Multiple reaction monitoring
Oligosaccharides
Legumes
Biología molecular
Alimentos
Molecular biology
Food
id ES_50e4c29d9d0c2d5a60db5f20d3fc2015
oai_identifier_str oai:riubu.ubu.es:10259/6196
network_acronym_str ES
network_name_str España
repository_id_str
spelling Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometerPrieto Santiago, VirginiaCavia Camarero, María del MarBarba, Francisco J.Alonso de la Torre, SaraCarrillo Pérez, CeliaTriple quadrupoleMultiple reaction monitoringOligosaccharidesLegumesBiología molecularAlimentosMolecular biologyFoodRaffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and quantification using liquid chromatography-tandem mass spectrometry equipped with a triple quadrupole analyser operating in Multiple Reaction Monitoring mode. Raffinose, stachyose and verbascose are separated in a 10-minute run and the method is validated over a broad concentration range, showing good linearity, accuracy, precision and high sensitivity. A low-cost, short eco-friendly procedure for oligosaccharide extraction from legumes, with a high recovery rate extraction, good repeatability and reproducibility is also proposed. No plant-matrix effects were demonstrated. The method applied to the screening of 28 different legumes revealed species-related traits for oligosaccharide distribution, highlighting Pisum sativum (9.22 g/100 g) as the richest source of these prebiotics and its suitability as a functional food ingredient.Prieto-Santiago is a research assistant funded by Junta de Castilla y León (Spain) (Grant No. UBU-10-B).Elsevier202120212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10259/6196reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)instname:Universidad de Burgos (UBU)InglésFood Chemistry. 2022, V. 368, 130761https://doi.org/10.1016/j.foodchem.2021.130761info:eu-repo/grantAgreement/Junta de Castilla y León//UBU-10-BAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riubu.ubu.es:10259/61962026-05-28T07:56:11Z
dc.title.none.fl_str_mv Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
title Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
spellingShingle Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
Prieto Santiago, Virginia
Triple quadrupole
Multiple reaction monitoring
Oligosaccharides
Legumes
Biología molecular
Alimentos
Molecular biology
Food
title_short Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
title_full Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
title_fullStr Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
title_full_unstemmed Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
title_sort Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
dc.creator.none.fl_str_mv Prieto Santiago, Virginia
Cavia Camarero, María del Mar
Barba, Francisco J.
Alonso de la Torre, Sara
Carrillo Pérez, Celia
author Prieto Santiago, Virginia
author_facet Prieto Santiago, Virginia
Cavia Camarero, María del Mar
Barba, Francisco J.
Alonso de la Torre, Sara
Carrillo Pérez, Celia
author_role author
author2 Cavia Camarero, María del Mar
Barba, Francisco J.
Alonso de la Torre, Sara
Carrillo Pérez, Celia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Triple quadrupole
Multiple reaction monitoring
Oligosaccharides
Legumes
Biología molecular
Alimentos
Molecular biology
Food
topic Triple quadrupole
Multiple reaction monitoring
Oligosaccharides
Legumes
Biología molecular
Alimentos
Molecular biology
Food
description Raffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and quantification using liquid chromatography-tandem mass spectrometry equipped with a triple quadrupole analyser operating in Multiple Reaction Monitoring mode. Raffinose, stachyose and verbascose are separated in a 10-minute run and the method is validated over a broad concentration range, showing good linearity, accuracy, precision and high sensitivity. A low-cost, short eco-friendly procedure for oligosaccharide extraction from legumes, with a high recovery rate extraction, good repeatability and reproducibility is also proposed. No plant-matrix effects were demonstrated. The method applied to the screening of 28 different legumes revealed species-related traits for oligosaccharide distribution, highlighting Pisum sativum (9.22 g/100 g) as the richest source of these prebiotics and its suitability as a functional food ingredient.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10259/6196
url http://hdl.handle.net/10259/6196
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Food Chemistry. 2022, V. 368, 130761
https://doi.org/10.1016/j.foodchem.2021.130761
info:eu-repo/grantAgreement/Junta de Castilla y León//UBU-10-B
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)
instname:Universidad de Burgos (UBU)
instname_str Universidad de Burgos (UBU)
reponame_str Repositorio Institucional de la Universidad de Burgos (RIUBU)
collection Repositorio Institucional de la Universidad de Burgos (RIUBU)
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407920830021632
score 15,301629

Similares en LA Referencia