Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)

This article belongs to the Special Issue Emerging Viruses: Surveillance, Prevention, Evolution and Control.

Detalles Bibliográficos
Autores: Águeda-Pinto, Ana, Lemos de Matos, Ana, Abrantes, Mário, Kraberger, Simona, Risalde, María Ángeles, Gortázar, Christian, McFadden, Grant, Varsani, Arvind, Esteves, Pedro J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215590
Acceso en línea:http://hdl.handle.net/10261/215590
Access Level:acceso abierto
Palabra clave:Poxvirus
Myxoma virus
Recombinant virus
Lepus granatensis
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spelling Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)Águeda-Pinto, AnaLemos de Matos, AnaAbrantes, MárioKraberger, SimonaRisalde, María ÁngelesGortázar, ChristianMcFadden, GrantVarsani, ArvindEsteves, Pedro J.PoxvirusMyxoma virusRecombinant virusLepus granatensisThis article belongs to the Special Issue Emerging Viruses: Surveillance, Prevention, Evolution and Control.Myxomatosis is a lethal disease in wild European and domestic rabbits (Oryctolagus cuniculus), which is caused by a Myxoma virus (MYXV) infection—a leporipoxvirus that is found naturally in some Sylvilagus rabbit species in South America and California. The introduction of MYXV into feral European rabbit populations of Australia and Europe, in the early 1950s, demonstrated the best-documented field example of host–virus coevolution, following a cross-species transmission. Recently, a new cross-species jump of MYXV has been suggested in both Great Britain and Spain, where European brown hares (Lepus europaeus) and Iberian hares (Lepus granatensis) were found dead with lesions consistent with those observed in myxomatosis. To investigate the possibility of a new cross-species transmission event by MYXV, tissue samples collected from a wild Iberian hare found dead in Spain (Toledo region) were analyzed and deep sequenced. Our results reported a new MYXV isolate (MYXV Toledo) in the tissues of this species. The genome of this new virus was found to encode three disruptive genes (M009L, M036L, and M152R) and a novel ~2.8 kb recombinant region, which resulted from an insertion of four novel poxviral genes towards the 3’ end of the negative strand of its genome. From the open reading frames inserted into the MYXV Toledo virus, a new orthologue of a poxvirus host range gene family member was identified, which was related to the MYXV gene M064R. Overall, we confirmed the identity of a new MYXV isolate in Iberian hares, which, we hypothesized, was able to more effectively counteract the host defenses in hares and start an infectious process in this new host.According to EU and National legislation (2010/63/UE Directive, the Spanish Royal Decree (53/2013), and the University of Castilla–La Mancha guidelines, no permission or consent was required to conduct the research reported herein. FCT—Foundation for Science and Technology supported the doctoral fellowship of A.A.-P. (ref. SFRH/BD/128752/2017) and the investigator grant of P.J.E. (IF/00376/2015). This article is a result of the project NORTE-01-0145-FEDER-000007, supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was also supported by the National Institute of Health (NIH) grant R01 A1080607.Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionUniversidad de Castilla La ManchaFundação para a Ciência e a Tecnologia (Portugal)Programa Operacional do Potencial Humano (Portugal)National Institutes of Health (US)Risalde, María Ángeles [0000-0001-6751-1305]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215590reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/v11060530Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2155902026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
title Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
spellingShingle Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
Águeda-Pinto, Ana
Poxvirus
Myxoma virus
Recombinant virus
Lepus granatensis
title_short Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
title_full Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
title_fullStr Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
title_full_unstemmed Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
title_sort Genetic characterization of a recombinant myxoma virus in the Iberian hare (Lepus granatensis)
dc.creator.none.fl_str_mv Águeda-Pinto, Ana
Lemos de Matos, Ana
Abrantes, Mário
Kraberger, Simona
Risalde, María Ángeles
Gortázar, Christian
McFadden, Grant
Varsani, Arvind
Esteves, Pedro J.
author Águeda-Pinto, Ana
author_facet Águeda-Pinto, Ana
Lemos de Matos, Ana
Abrantes, Mário
Kraberger, Simona
Risalde, María Ángeles
Gortázar, Christian
McFadden, Grant
Varsani, Arvind
Esteves, Pedro J.
author_role author
author2 Lemos de Matos, Ana
Abrantes, Mário
Kraberger, Simona
Risalde, María Ángeles
Gortázar, Christian
McFadden, Grant
Varsani, Arvind
Esteves, Pedro J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Universidad de Castilla La Mancha
Fundação para a Ciência e a Tecnologia (Portugal)
Programa Operacional do Potencial Humano (Portugal)
National Institutes of Health (US)
Risalde, María Ángeles [0000-0001-6751-1305]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Poxvirus
Myxoma virus
Recombinant virus
Lepus granatensis
topic Poxvirus
Myxoma virus
Recombinant virus
Lepus granatensis
description This article belongs to the Special Issue Emerging Viruses: Surveillance, Prevention, Evolution and Control.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215590
url http://hdl.handle.net/10261/215590
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/v11060530
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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