Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers

For patients with type 2 diabetes, metformin is the most often recommended drug. However, there are substantial individual differences in the pharmacological response to metformin. To investigate the effect of transporter polymorphisms on metformin pharmacokinetics in an environment free of confound...

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Autores: Saiz Rodríguez, Miriam, Ochoa Mazarro, María Dolores, Zubiaur Precioso, Pablo, Navares-Gómez, Marcos, Román, Manuel, Camargo-Mamani, Paola, Luquero-Bueno, Sergio, Villapalos-García, Gonzalo, Alcaraz, Raquel, Mejía Abril, Gina, Santos-Mazo, Estefanía, Abad Santos, Francisco
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/708739
Acceso en línea:http://hdl.handle.net/10486/708739
https://dx.doi.org/10.3390/jpm13030489
Access Level:acceso abierto
Palabra clave:metformin
pharmacogenetics
transporters
SLC
ABC
sex
ethnicity
Medicina
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spelling Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteersSaiz Rodríguez, MiriamOchoa Mazarro, María DoloresZubiaur Precioso, PabloNavares-Gómez, MarcosRomán, ManuelCamargo-Mamani, PaolaLuquero-Bueno, SergioVillapalos-García, GonzaloAlcaraz, RaquelMejía Abril, GinaSantos-Mazo, EstefaníaAbad Santos, FranciscometforminpharmacogeneticstransportersSLCABCsexethnicityMedicinaFor patients with type 2 diabetes, metformin is the most often recommended drug. However, there are substantial individual differences in the pharmacological response to metformin. To investigate the effect of transporter polymorphisms on metformin pharmacokinetics in an environment free of confounding variables, we conducted our study on healthy participants. This is the first investigation to consider demographic characteristics alongside all transporters involved in metformin distribution. Pharmacokinetic parameters of metformin were found to be affected by age, sex, ethnicity, and several polymorphisms. Age and SLC22A4 and SLC47A2 polymorphisms affected the area under the concentration-time curve (AUC). However, after adjusting for dose-to-weight ratio (dW), sex, age, and ethnicity, along with SLC22A3 and SLC22A4, influenced AUC. The maximum concentration was affected by age and SLC22A1, but after adjusting for dW, it was affected by sex, age, ethnicity, ABCG2, and SLC22A4. The time to reach the maximum concentration was influenced by sex, like half-life, which was also affected by SLC22A3. The volume of distribution and clearance was affected by sex, age, ethnicity and SLC22A3. Alternatively, the pharmacokinetics of metformin was unaffected by polymorphisms in ABCB1, SLC2A2, SLC22A2, or SLC47A1. Therefore, our study demonstrates that a multifactorial approach to all patient characteristics is necessary for better individualizationThe project was financed by the Regional Health Management of Castilla y León (GRS 2432/A/21) and partially by Fundación Burgos por la Investigación de la Salud (FBIS). M.S.R. research is supported by Instituto de Salud Carlos III (ISCIII), Spanish Ministry of Science and Innovation, through the Sara Borrell Program (CD21/00022). G.V.G. is cofinanced by Instituto de Salud Carlos III (ISCIII) and the European Social Fund (PFIS predoctoral grant, number FI20/00090). M.N.G. is financed by the ICI20/00131 grant, Acción Estratégica en Salud 2017–2020, ISCIII. P.Z. is financed by Universidad Autónoma de Madrid, Margarita Salas contract, grants for the requalification of the Spanish university system. The MassArray genotyping service was carried out at the Spanish genotyping center–Centro Español de Genotipado CEGEN-PRB3-ISCIII, which is supported by grant PT17/0019 of the PE I+D+i 2013–2016, funded by ISCIII and European Regional Development Fund ERDFMDPIDepartamento de FarmacologíaFacultad de Medicina20232023-03-08research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/708739https://dx.doi.org/10.3390/jpm13030489reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7087392026-06-23T12:46:27Z
dc.title.none.fl_str_mv Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
title Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
spellingShingle Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
Saiz Rodríguez, Miriam
metformin
pharmacogenetics
transporters
SLC
ABC
sex
ethnicity
Medicina
title_short Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
title_full Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
title_fullStr Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
title_full_unstemmed Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
title_sort Identification of transporter polymorphisms influencing metformin pharmacokinetics in healthy volunteers
dc.creator.none.fl_str_mv Saiz Rodríguez, Miriam
Ochoa Mazarro, María Dolores
Zubiaur Precioso, Pablo
Navares-Gómez, Marcos
Román, Manuel
Camargo-Mamani, Paola
Luquero-Bueno, Sergio
Villapalos-García, Gonzalo
Alcaraz, Raquel
Mejía Abril, Gina
Santos-Mazo, Estefanía
Abad Santos, Francisco
author Saiz Rodríguez, Miriam
author_facet Saiz Rodríguez, Miriam
Ochoa Mazarro, María Dolores
Zubiaur Precioso, Pablo
Navares-Gómez, Marcos
Román, Manuel
Camargo-Mamani, Paola
Luquero-Bueno, Sergio
Villapalos-García, Gonzalo
Alcaraz, Raquel
Mejía Abril, Gina
Santos-Mazo, Estefanía
Abad Santos, Francisco
author_role author
author2 Ochoa Mazarro, María Dolores
Zubiaur Precioso, Pablo
Navares-Gómez, Marcos
Román, Manuel
Camargo-Mamani, Paola
Luquero-Bueno, Sergio
Villapalos-García, Gonzalo
Alcaraz, Raquel
Mejía Abril, Gina
Santos-Mazo, Estefanía
Abad Santos, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Farmacología
Facultad de Medicina
dc.subject.none.fl_str_mv metformin
pharmacogenetics
transporters
SLC
ABC
sex
ethnicity
Medicina
topic metformin
pharmacogenetics
transporters
SLC
ABC
sex
ethnicity
Medicina
description For patients with type 2 diabetes, metformin is the most often recommended drug. However, there are substantial individual differences in the pharmacological response to metformin. To investigate the effect of transporter polymorphisms on metformin pharmacokinetics in an environment free of confounding variables, we conducted our study on healthy participants. This is the first investigation to consider demographic characteristics alongside all transporters involved in metformin distribution. Pharmacokinetic parameters of metformin were found to be affected by age, sex, ethnicity, and several polymorphisms. Age and SLC22A4 and SLC47A2 polymorphisms affected the area under the concentration-time curve (AUC). However, after adjusting for dose-to-weight ratio (dW), sex, age, and ethnicity, along with SLC22A3 and SLC22A4, influenced AUC. The maximum concentration was affected by age and SLC22A1, but after adjusting for dW, it was affected by sex, age, ethnicity, ABCG2, and SLC22A4. The time to reach the maximum concentration was influenced by sex, like half-life, which was also affected by SLC22A3. The volume of distribution and clearance was affected by sex, age, ethnicity and SLC22A3. Alternatively, the pharmacokinetics of metformin was unaffected by polymorphisms in ABCB1, SLC2A2, SLC22A2, or SLC47A1. Therefore, our study demonstrates that a multifactorial approach to all patient characteristics is necessary for better individualization
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-03-08
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/708739
https://dx.doi.org/10.3390/jpm13030489
url http://hdl.handle.net/10486/708739
https://dx.doi.org/10.3390/jpm13030489
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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