Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease

12 p.

Detalles Bibliográficos
Autores: Campillo De Blas, Sofía, Gutiérrez Calabrés, Elena|||0000-0001-8144-6909, García Miranda, Susana, Griera Merino, Mercedes|||0000-0002-9887-6638, Fernández Rodríguez, María Loreto, Frutos García, Sergio De|||0000-0002-1225-5911, Rodríguez Puyol, Diego María|||0000-0002-9125-9311, Calleros Basilio, Laura|||0000-0001-7739-1319
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:español
OAI Identifier:oai:ebuah.uah.es:10017/61868
Acceso en línea:http://hdl.handle.net/10017/61868
https://dx.doi.org/10.1186/s12964-024-01646-2
Access Level:acceso abierto
Palabra clave:ILK
Biomarker
Renal damage
Vascular damage
Chronic kidney disease (CKD)
Medicina
Medicine
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spelling Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney diseaseCampillo De Blas, SofíaGutiérrez Calabrés, Elena|||0000-0001-8144-6909García Miranda, SusanaGriera Merino, Mercedes|||0000-0002-9887-6638Fernández Rodríguez, María LoretoFrutos García, Sergio De|||0000-0002-1225-5911Rodríguez Puyol, Diego María|||0000-0002-9125-9311Calleros Basilio, Laura|||0000-0001-7739-1319ILKBiomarkerRenal damageVascular damageChronic kidney disease (CKD)MedicinaMedicine12 p.Background: Traditional biomarkers of chronic kidney disease (CKD) detect the disease in its late stages and hardly predict associated vascular damage. Integrin-linked kinase (ILK) is a scaffolding protein and a serine/threonine protein kinase that plays multiple roles in several pathophysiological processes during renal damage. However, the involvement of ILK as a biomarker of CKD and its associated vascular problems remains to be fully elucidated. Methods: CKD was induced by an adenine-rich diet for 6 weeks in mice. We used an inducible ILK knockdown mice (cKD-ILK) model to decrease ILK expression. ILK content in mice's peripheral blood mononuclear cells (PBMCs) was determined and correlated with renal function parameters and with the expression of ILK and fibrosis and inflammation markers in renal and aortic tissues. Also, the expression of five miRNAs that target ILK was analyzed in whole blood of mice. Results: The adenine diet increased ILK expression in PBMCs, renal cortex, and aortas, and creatinine and urea nitrogen concentrations in the plasma of WT mice, while these increases were not observed in cKD-ILK mice. Furthermore, ILK content in PBMCs directly correlated with renal function parameters and with the expression of renal and vascular ILK and fibrosis and inflammation markers. Finally, the expression of the five miRNAs increased in the whole blood of adenine-fed mice, although only four correlated with plasma urea nitrogen, and of those, three were downregulated in cKD-ILK mice. Conclusions: ILK, in circulating mononuclear cells, could be a potential biomarker of CKD and CKD-associated renal and vascular damage.Instituto de Salud Carlos IIIFEDERComunidad de MadridUniversidad de AlcaláFundación Renal Íñigo Álvarez de Toledo (FRIAT)20242024-05-11journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/61868https://dx.doi.org/10.1186/s12964-024-01646-2reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)EspañolspaNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableNot available Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/618682026-06-18T11:13:07Z
dc.title.none.fl_str_mv Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
title Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
spellingShingle Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
Campillo De Blas, Sofía
ILK
Biomarker
Renal damage
Vascular damage
Chronic kidney disease (CKD)
Medicina
Medicine
title_short Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
title_full Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
title_fullStr Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
title_full_unstemmed Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
title_sort Integrin-linked kinase mRNA expression in circulating mononuclear cells as a biomarker of kidney and vascular damage in experimental chronic kidney disease
dc.creator.none.fl_str_mv Campillo De Blas, Sofía
Gutiérrez Calabrés, Elena|||0000-0001-8144-6909
García Miranda, Susana
Griera Merino, Mercedes|||0000-0002-9887-6638
Fernández Rodríguez, María Loreto
Frutos García, Sergio De|||0000-0002-1225-5911
Rodríguez Puyol, Diego María|||0000-0002-9125-9311
Calleros Basilio, Laura|||0000-0001-7739-1319
author Campillo De Blas, Sofía
author_facet Campillo De Blas, Sofía
Gutiérrez Calabrés, Elena|||0000-0001-8144-6909
García Miranda, Susana
Griera Merino, Mercedes|||0000-0002-9887-6638
Fernández Rodríguez, María Loreto
Frutos García, Sergio De|||0000-0002-1225-5911
Rodríguez Puyol, Diego María|||0000-0002-9125-9311
Calleros Basilio, Laura|||0000-0001-7739-1319
author_role author
author2 Gutiérrez Calabrés, Elena|||0000-0001-8144-6909
García Miranda, Susana
Griera Merino, Mercedes|||0000-0002-9887-6638
Fernández Rodríguez, María Loreto
Frutos García, Sergio De|||0000-0002-1225-5911
Rodríguez Puyol, Diego María|||0000-0002-9125-9311
Calleros Basilio, Laura|||0000-0001-7739-1319
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ILK
Biomarker
Renal damage
Vascular damage
Chronic kidney disease (CKD)
Medicina
Medicine
topic ILK
Biomarker
Renal damage
Vascular damage
Chronic kidney disease (CKD)
Medicina
Medicine
description 12 p.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-05-11
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/61868
https://dx.doi.org/10.1186/s12964-024-01646-2
url http://hdl.handle.net/10017/61868
https://dx.doi.org/10.1186/s12964-024-01646-2
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.relation.none.fl_str_mv Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
Not available Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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