Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection

Distinct lineages of the grasshopper Chorthippus parallelus (Orthoptera: Acrididae) form well-known hybrid zones (HZs) both in the Pyrenees and the Alps mountain ranges in South Europe. These HZs represent unique experimental systems to identify “key genes” that maintain genetic boundaries between e...

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Autores: Jiménez Florido, Patricia, Aquilino, Mónica, Buckley Iglesias, David, Bella Sombría, José Luis, Planelló Carro, Rosario
Tipo de documento: artigo
Data de publicação:2024
País:España
Recursos:Universidad Autónoma de Madrid
Repositório:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglês
OAI Identifier:oai:repositorio.uam.es:10486/716750
Acesso em linha:http://hdl.handle.net/10486/716750
https://dx.doi.org/10.1111/1744-7917.13481
Access Level:Acceso aberto
Palavra-chave:Energy Metabolism
Hybrid Zones
Immune System Response
Insects
Repro-duction
Wolbachia
Biología y Biomedicina / Biología
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spelling Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infectionJiménez Florido, PatriciaAquilino, MónicaBuckley Iglesias, DavidBella Sombría, José LuisPlanelló Carro, RosarioEnergy MetabolismHybrid ZonesImmune System ResponseInsectsRepro-ductionWolbachiaBiología y Biomedicina / BiologíaDistinct lineages of the grasshopper Chorthippus parallelus (Orthoptera: Acrididae) form well-known hybrid zones (HZs) both in the Pyrenees and the Alps mountain ranges in South Europe. These HZs represent unique experimental systems to identify “key genes” that maintain genetic boundaries between emerging species. The Iberian endemism C. p. erythropus (Cpe) and the subspecies C. p. parallelus (Cpp), widely distributed throughout the rest of Europe, overlap and form the Pyrenean HZ. Both subspeciesdiffer morphologically, as well as in behavioral, mitochondrial, nuclear, and chromosomal traits, and in the strains of the maternally transmitted bacterial endosymbiont Wolbachia infecting them. This results in either unidirectional and bidirectional cytoplasmic incom-patibility between both grasshopper subspecies, pointing out that Wolbachia clearly affects gene expression in the infected individuals. Here we explore how Wolbachia may modify the expression of some major genes involved in relevant pathways in Cpp in the Pyrenean HZ. We have analyzed, through molecular biomarkers, the physiological response sin C. parallelus individuals infected by Wolbachia, with particular attention to the energy metabolism, the immune system response, and the reproduction. qPCR was used to evaluate the expression of selected genes in the gonads of infected and uninfected adults of both sexes, since this tissue constitutes the main target of Wolbachia infection. Transcrip-tional analyses also showed differential sex-dependent responses in most of the analyzedbiomarkers in infected and noninfected individuals. We identified for the first time newsensitive biomarkers that might be involved in the reproductive barrier induced by Wol-bachia in the hybrid zoneThis Research was supported by the Spanish Ministerio de Economía y Competitividad (MINECO) through the research project I+D+i PID2019-104952GBI00. Mónica Aquilino was cofinanced by the Comunidad de Madrid and the European Social Fund with a postdoctoral contract (PEJD-2019-POST/AMB-16425), and she is currently financedby the Spanish Ministerio de Universidades and the European UnionNextGeneration Programme with a María Zambrano grant. Patricia Jiménez-Florido currently enjoys the FPU contract 22/02220 of the Spanish Ministerio de Ciencia, Innovación y UniversidadesWileyDepartamento de BiologíaFacultad de Ciencias20242024-11-29research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/716750https://dx.doi.org/10.1111/1744-7917.13481reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7167502026-06-23T12:46:27Z
dc.title.none.fl_str_mv Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
title Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
spellingShingle Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
Jiménez Florido, Patricia
Energy Metabolism
Hybrid Zones
Immune System Response
Insects
Repro-duction
Wolbachia
Biología y Biomedicina / Biología
title_short Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
title_full Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
title_fullStr Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
title_full_unstemmed Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
title_sort Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection
dc.creator.none.fl_str_mv Jiménez Florido, Patricia
Aquilino, Mónica
Buckley Iglesias, David
Bella Sombría, José Luis
Planelló Carro, Rosario
author Jiménez Florido, Patricia
author_facet Jiménez Florido, Patricia
Aquilino, Mónica
Buckley Iglesias, David
Bella Sombría, José Luis
Planelló Carro, Rosario
author_role author
author2 Aquilino, Mónica
Buckley Iglesias, David
Bella Sombría, José Luis
Planelló Carro, Rosario
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología
Facultad de Ciencias
dc.subject.none.fl_str_mv Energy Metabolism
Hybrid Zones
Immune System Response
Insects
Repro-duction
Wolbachia
Biología y Biomedicina / Biología
topic Energy Metabolism
Hybrid Zones
Immune System Response
Insects
Repro-duction
Wolbachia
Biología y Biomedicina / Biología
description Distinct lineages of the grasshopper Chorthippus parallelus (Orthoptera: Acrididae) form well-known hybrid zones (HZs) both in the Pyrenees and the Alps mountain ranges in South Europe. These HZs represent unique experimental systems to identify “key genes” that maintain genetic boundaries between emerging species. The Iberian endemism C. p. erythropus (Cpe) and the subspecies C. p. parallelus (Cpp), widely distributed throughout the rest of Europe, overlap and form the Pyrenean HZ. Both subspeciesdiffer morphologically, as well as in behavioral, mitochondrial, nuclear, and chromosomal traits, and in the strains of the maternally transmitted bacterial endosymbiont Wolbachia infecting them. This results in either unidirectional and bidirectional cytoplasmic incom-patibility between both grasshopper subspecies, pointing out that Wolbachia clearly affects gene expression in the infected individuals. Here we explore how Wolbachia may modify the expression of some major genes involved in relevant pathways in Cpp in the Pyrenean HZ. We have analyzed, through molecular biomarkers, the physiological response sin C. parallelus individuals infected by Wolbachia, with particular attention to the energy metabolism, the immune system response, and the reproduction. qPCR was used to evaluate the expression of selected genes in the gonads of infected and uninfected adults of both sexes, since this tissue constitutes the main target of Wolbachia infection. Transcrip-tional analyses also showed differential sex-dependent responses in most of the analyzedbiomarkers in infected and noninfected individuals. We identified for the first time newsensitive biomarkers that might be involved in the reproductive barrier induced by Wol-bachia in the hybrid zone
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-11-29
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/716750
https://dx.doi.org/10.1111/1744-7917.13481
url http://hdl.handle.net/10486/716750
https://dx.doi.org/10.1111/1744-7917.13481
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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