PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway

37 p.-6 fig.

Detalles Bibliográficos
Autores: Escudero-Paniagua, B., Bartolomé, Rubén Álvaro, Rodríguez, Sandra, Ríos, Vivian de los, Pintado-Berninches, Laura, Jaén, Marta, Lafarga, Miguel, Fernandez-Aceñero, M. Jesús, Casal, J. Ignacio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/182047
Acceso en línea:http://hdl.handle.net/10261/182047
Access Level:acceso abierto
Palabra clave:PAUF/ZG16B
Mitosis
Vesicle trafficking
Wnt/ β-catenin
Colorectal cancer progression
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spelling PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathwayEscudero-Paniagua, B.Bartolomé, Rubén ÁlvaroRodríguez, SandraRíos, Vivian de losPintado-Berninches, LauraJaén, MartaLafarga, MiguelFernandez-Aceñero, M. JesúsCasal, J. IgnacioPAUF/ZG16BMitosisVesicle traffickingWnt/ β-cateninColorectal cancer progression37 p.-6 fig.Pancreatic adenocarcinoma–upregulated factor (PAUF), also known as ZG16B, was previously found in the secretome of metastatic colorectal cancer cells. Here, we demonstrated the presence of PAUF at the intracellular level and its multiple effects on cancer progression. An initial decline of PAUF expression was observed at early stages of colorectal cancer followed by an increase at the metastastic site. PAUF was located at different cellular compartments: membrane-associated vesicles, endosomes, microtubule-associated vesicles, cell growth cones and the cell nucleus. PAUF loss in two colorectal cancer cell lines caused severe alterations in the cell phenotype and cell cycle, including tetraploidy, extensive genomic alterations, micronuclei, and increased apoptosis. An exhaustive analysis of the PAUF interactome using different proteomic approaches revealed the presence of multiple components of the cell cycle, mitotic checkpoint, Wnt pathway and intracellular transport. Among the interacting proteins we found ZW10, a moonlighting protein with a dual function in membrane trafficking and mitosis. In addition, PAUF silencing was associated to APC loss and increased β-catenin nuclear expression. Altogether, our results suggest that PAUF depletion increases aneuploidy, promotes apoptosis and activates the Wnt/β-catenin pathway in colorectal cancer cells facilitating cancer progression. In summary, PAUF behaves as a multifunctional protein, with different roles in cancer progression according to the extra- or intracellular expression, suggesting a therapeutic value for colorectal cancer.This research was supported by grants from the MINECO (BIO2015-66489-R), Fundación Areces and PRB3 (IPT17/0019 - ISCIII-SGEFI /ERDF).Peer reviewedOxford University PressMinisterio de Economía y Competitividad (España)Fundación Ramón ArecesEscudero-Paniagua, B. [0000-0003-3882-5529]Bartolomé, Rubén Álvaro [0000-0002-3292-1491]de los Ríos, Vivian [0000-0001-5582-6879]Lafarga, Miguel [0000-0003-3402-1152]Fernandez-Aceñero, M. Jesús [0000-0002-2439-3553]Casal, J. Ignacio [0000-0003-1085-2840]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/182047reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-66489-Rhttps://doi.org/10.1093/carcin/bgz093Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1820472026-05-22T06:33:51Z
dc.title.none.fl_str_mv PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
title PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
spellingShingle PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
Escudero-Paniagua, B.
PAUF/ZG16B
Mitosis
Vesicle trafficking
Wnt/ β-catenin
Colorectal cancer progression
title_short PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
title_full PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
title_fullStr PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
title_full_unstemmed PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
title_sort PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway
dc.creator.none.fl_str_mv Escudero-Paniagua, B.
Bartolomé, Rubén Álvaro
Rodríguez, Sandra
Ríos, Vivian de los
Pintado-Berninches, Laura
Jaén, Marta
Lafarga, Miguel
Fernandez-Aceñero, M. Jesús
Casal, J. Ignacio
author Escudero-Paniagua, B.
author_facet Escudero-Paniagua, B.
Bartolomé, Rubén Álvaro
Rodríguez, Sandra
Ríos, Vivian de los
Pintado-Berninches, Laura
Jaén, Marta
Lafarga, Miguel
Fernandez-Aceñero, M. Jesús
Casal, J. Ignacio
author_role author
author2 Bartolomé, Rubén Álvaro
Rodríguez, Sandra
Ríos, Vivian de los
Pintado-Berninches, Laura
Jaén, Marta
Lafarga, Miguel
Fernandez-Aceñero, M. Jesús
Casal, J. Ignacio
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Ramón Areces
Escudero-Paniagua, B. [0000-0003-3882-5529]
Bartolomé, Rubén Álvaro [0000-0002-3292-1491]
de los Ríos, Vivian [0000-0001-5582-6879]
Lafarga, Miguel [0000-0003-3402-1152]
Fernandez-Aceñero, M. Jesús [0000-0002-2439-3553]
Casal, J. Ignacio [0000-0003-1085-2840]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PAUF/ZG16B
Mitosis
Vesicle trafficking
Wnt/ β-catenin
Colorectal cancer progression
topic PAUF/ZG16B
Mitosis
Vesicle trafficking
Wnt/ β-catenin
Colorectal cancer progression
description 37 p.-6 fig.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/182047
url http://hdl.handle.net/10261/182047
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-66489-R
https://doi.org/10.1093/carcin/bgz093
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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