The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS

30 p.-10 fig.-1 tab.

Detalles Bibliográficos
Autores: Manzano, Aranzazu, Creus, Eva, Tomás, Albert, Valbuena, Miguel A., Villacampa, Alicia, Ciska, Malgorzata, Edelmann, Richard E., Kiss, John Z., Medina, F. Javier, Herranz, Raúl
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/221282
Acceso en línea:http://hdl.handle.net/10261/221282
Access Level:acceso abierto
Palabra clave:Microgravity
Plant
Spaceflight
Hardware
Preservation
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oai_identifier_str oai:digital.csic.es:10261/221282
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
title The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
spellingShingle The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
Manzano, Aranzazu
Microgravity
Plant
Spaceflight
Hardware
Preservation
title_short The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
title_full The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
title_fullStr The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
title_full_unstemmed The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
title_sort The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISS
dc.creator.none.fl_str_mv Manzano, Aranzazu
Creus, Eva
Tomás, Albert
Valbuena, Miguel A.
Villacampa, Alicia
Ciska, Malgorzata
Edelmann, Richard E.
Kiss, John Z.
Medina, F. Javier
Herranz, Raúl
author Manzano, Aranzazu
author_facet Manzano, Aranzazu
Creus, Eva
Tomás, Albert
Valbuena, Miguel A.
Villacampa, Alicia
Ciska, Malgorzata
Edelmann, Richard E.
Kiss, John Z.
Medina, F. Javier
Herranz, Raúl
author_role author
author2 Creus, Eva
Tomás, Albert
Valbuena, Miguel A.
Villacampa, Alicia
Ciska, Malgorzata
Edelmann, Richard E.
Kiss, John Z.
Medina, F. Javier
Herranz, Raúl
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
NASA Astrobiology Institute
European Space Agency
Manzano, Aranzazu [0000-0002-0150-0803]
Tomás, Albert [0000-0001-9835-8825]
Valbuena, Miguel A. [0000-0002-0585-5636]
Villacampa, Alicia [0000-0002-7398-8545]
Ciska, Malgorzata [0000-0002-6514-9493]
Edelmann, Richard E. [0000-0002-8316-526X]
Kiss, John Z. [0000-0003-1326-127X]
Medina, F. Javier [0000-0002-0866-7710]
Herranz, Raúl [0000-0002-0246-9449]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Microgravity
Plant
Spaceflight
Hardware
Preservation
topic Microgravity
Plant
Spaceflight
Hardware
Preservation
description 30 p.-10 fig.-1 tab.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/221282
url http://hdl.handle.net/10261/221282
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/ESP2015–64323-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018–099309-B-I00
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BES-2013-063933
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BES-2016-077976
https://doi.org/10.1007/s12217-020-09837-5
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling The FixBox: hardware to provide on-orbit fixation capabilities to the EMCS on the ISSManzano, AranzazuCreus, EvaTomás, AlbertValbuena, Miguel A.Villacampa, AliciaCiska, MalgorzataEdelmann, Richard E.Kiss, John Z.Medina, F. JavierHerranz, RaúlMicrogravityPlantSpaceflightHardwarePreservation30 p.-10 fig.-1 tab.Plant biology is an important area for the future of space exploration, but biological spaceflight experiments have been always constrained by the hardware capabilities. The European Modular Cultivation System (EMCS) unit was an incubator for small organisms, such as Arabidopsis thaliana, built by the European Space Agency (ESA) and was decommissioned in 2018. Here, we describe the FixBox system as add-on hardware to provide fixation capabilities to the plant growth cassettes, which, initially, were not designed to be used for that purpose. Tests were performed to ensure the successful use of this device in the EMCS facility. We also evaluate the required adaptations to the hardware, e.g., to guarantee that the reduced fluid motion in microgravity does not cause any bubbles that could impair the quality of fixation. Arabidopsis thaliana seedlings grown during spaceflight were fixed in the FixBox either in glutaraldehyde or formaldehyde. Electron microscopical images and confocal microscopy immunofluorescent localizations showed an excellent preservation of both cell ultrastructure and antigen conformation. Thus, it is possible to modify existing hardware to comply with the scientific requirements to augment the existing capabilities on board the ISS. In addition, it is also possible to reuse culture chambers from predesigned experimental containers into new modular subunits as FixBox. Similarly, we can design new hardware compatible with a novel cultivation chamber on board, such as is available in BIOLAB, to be used later with FixBox. Lessons learned for future space plant biology researchers include how to manage the number of hardware requirements and constraints on how to preserve the biological samples.This work was supported by the Agencia Estatal de Investigación of the Spanish Ministry of Science an Innovation, Grants #ESP2015–64323-R and #RTI2018–099309-B-I00 (co-funded by EU-ERDF), awarded to FJM, and by pre-doctoral fellowships to (AM), (MV) and (AV) from the Spanish National Program for Young Researchers Training (MINECO, Ref. BES-2013-063933, BES-2016-077976). This research was supported also by grants (NNX12A0656 and 80NSSC17K0546) from NASA to JZK. The Seedling Growth Project spaceflights and activities to the ISS were funded by ILSRA2009–0932/1177 of ESA-ELIPS Program. The FixBox has been built under a contract to SENER Aeroespacial SA by ESA (4000105697/12/NL/FC) to support the SG project.Peer reviewedSpringer NatureMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)NASA Astrobiology InstituteEuropean Space AgencyManzano, Aranzazu [0000-0002-0150-0803]Tomás, Albert [0000-0001-9835-8825]Valbuena, Miguel A. [0000-0002-0585-5636]Villacampa, Alicia [0000-0002-7398-8545]Ciska, Malgorzata [0000-0002-6514-9493]Edelmann, Richard E. [0000-0002-8316-526X]Kiss, John Z. [0000-0003-1326-127X]Medina, F. Javier [0000-0002-0866-7710]Herranz, Raúl [0000-0002-0246-9449]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/221282reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/ESP2015–64323-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018–099309-B-I00info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BES-2013-063933info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BES-2016-077976https://doi.org/10.1007/s12217-020-09837-5Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2212822026-05-22T06:33:51Z
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