The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection

This work describes, for the first time, the changes taking place in the antioxidative system of the leaf apoplast in response to Plum pox virus (PPV) in different Prunus species showing different susceptibilities to PPV. The presence of p-hydroxy mercury benzoic acid (pHMB)-sensitive ascorbate pero...

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Autores: Díaz-Vivancos, Pedro, Rubio, Manuel, Mesonero, Víctor, Periago, Paula María, Ros Barceló, Alfonso, Martínez-Gómez, Pedro, Hernández, José Antonio
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/4971
Acceso en línea:http://hdl.handle.net/10261/4971
Access Level:acceso abierto
Palabra clave:Apoplast
Apricot
peach
Breeding
2D Electrophoresis
germplasm
Sharka
oxidative stress
resistance
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spelling The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infectionDíaz-Vivancos, PedroRubio, ManuelMesonero, VíctorPeriago, Paula MaríaRos Barceló, AlfonsoMartínez-Gómez, PedroHernández, José AntonioApoplastApricotpeachBreeding2D ElectrophoresisgermplasmSharkaoxidative stressresistanceThis work describes, for the first time, the changes taking place in the antioxidative system of the leaf apoplast in response to Plum pox virus (PPV) in different Prunus species showing different susceptibilities to PPV. The presence of p-hydroxy mercury benzoic acid (pHMB)-sensitive ascorbate peroxidase (APX) (Class I APX) and pHMB-insensitive APX (Class III APX), superoxide dismutase (SOD), peroxidases (POX), NADH-POX and polyphenoloxidase (PPO) was described in the apoplast from both peach and apricot leaves. PPV infection produced different changes in the antioxidant system of the leaf apoplast from the Prunus species, depending on their susceptibility to the virus. In leaves of the very susceptible peach cultivar GF305, PPV brought about an increase in Class I APX, POX, NADH-POX and PPO activities. In the susceptible apricot cultivar Real Fino, PPV infection produced a decrease in apoplastic POX and SOD activities, whereas a strong increase in PPO was observed. However, in the resistant apricot cultivar Stark Early Orange, a rise in Class I APX as well as a strong increase in POX and SOD activities was noticed in the apoplastic compartment. Long-term PPV infection produced an oxidative stress in the apoplastic space from apricot and peach plants, as observed by the increase in H2O2 contents in this compartment. However, this increase was much higher in the PPV-susceptible plants than in the resistant apricot cultivar. Only in the PPV-susceptible apricot and peach plants was the increase in apoplastic H2O2 levels accompanied by an increase in electrolyte leakage. No changes in the electrolyte leakage were observed in the PPV-inoculated resistant apricot leaves, although a 42% increase in the apoplastic H2O2 levels was produced. Two-dimensional electrophoresis analyses revealed that the majority of the polypeptides in the apoplastic fluid had isoelectric points in the range of pI 4-6. The identification of proteins using MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and peptide mass fingerprinting analyses showed the induction of a thaumatin-like protein as well as the decrease of mandelonitrile lyase in peach apoplast due to PPV infection. However, most of the selected polypeptides showed no homology with known proteins. This fact emphasizes that, at least in Prunus, most of the functions of the apoplastic space remain unknown. We conclude that long-term PPV infection produced an oxidative stress in the leaf apoplast, contributing to the deleterious effects produced by PPV infection in leaves of inoculated, susceptible Prunus plantsThis work has been supported by grant AGL-2002-02115 from the CICYT (Comisión Interministerial de Ciencia y Tecnología) of Spain and grant 23BIO2005-04-6444 from the Comunidad de Murcia (Spain). P.M. Periago thanks the Ministerio de Ciencia y Tecnología for her contract in the Programme “Ramón y Cajal”.Peer reviewedComisión Interministerial de Ciencia y Tecnología, CICYT (España)Gobierno de la Región de MurciaMinisterio de Ciencia y Tecnología (España)200820082008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501437751 bytesapplication/pdfhttp://hdl.handle.net/10261/4971reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésJournal of Experimental Botanyvol 57:3813-3824info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/49712026-05-22T06:33:51Z
dc.title.none.fl_str_mv The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
title The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
spellingShingle The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
Díaz-Vivancos, Pedro
Apoplast
Apricot
peach
Breeding
2D Electrophoresis
germplasm
Sharka
oxidative stress
resistance
title_short The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
title_full The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
title_fullStr The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
title_full_unstemmed The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
title_sort The apoplastic antioxidant system in Prunus: Response to long-term Plum pox virus infection
dc.creator.none.fl_str_mv Díaz-Vivancos, Pedro
Rubio, Manuel
Mesonero, Víctor
Periago, Paula María
Ros Barceló, Alfonso
Martínez-Gómez, Pedro
Hernández, José Antonio
author Díaz-Vivancos, Pedro
author_facet Díaz-Vivancos, Pedro
Rubio, Manuel
Mesonero, Víctor
Periago, Paula María
Ros Barceló, Alfonso
Martínez-Gómez, Pedro
Hernández, José Antonio
author_role author
author2 Rubio, Manuel
Mesonero, Víctor
Periago, Paula María
Ros Barceló, Alfonso
Martínez-Gómez, Pedro
Hernández, José Antonio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Gobierno de la Región de Murcia
Ministerio de Ciencia y Tecnología (España)
dc.subject.none.fl_str_mv Apoplast
Apricot
peach
Breeding
2D Electrophoresis
germplasm
Sharka
oxidative stress
resistance
topic Apoplast
Apricot
peach
Breeding
2D Electrophoresis
germplasm
Sharka
oxidative stress
resistance
description This work describes, for the first time, the changes taking place in the antioxidative system of the leaf apoplast in response to Plum pox virus (PPV) in different Prunus species showing different susceptibilities to PPV. The presence of p-hydroxy mercury benzoic acid (pHMB)-sensitive ascorbate peroxidase (APX) (Class I APX) and pHMB-insensitive APX (Class III APX), superoxide dismutase (SOD), peroxidases (POX), NADH-POX and polyphenoloxidase (PPO) was described in the apoplast from both peach and apricot leaves. PPV infection produced different changes in the antioxidant system of the leaf apoplast from the Prunus species, depending on their susceptibility to the virus. In leaves of the very susceptible peach cultivar GF305, PPV brought about an increase in Class I APX, POX, NADH-POX and PPO activities. In the susceptible apricot cultivar Real Fino, PPV infection produced a decrease in apoplastic POX and SOD activities, whereas a strong increase in PPO was observed. However, in the resistant apricot cultivar Stark Early Orange, a rise in Class I APX as well as a strong increase in POX and SOD activities was noticed in the apoplastic compartment. Long-term PPV infection produced an oxidative stress in the apoplastic space from apricot and peach plants, as observed by the increase in H2O2 contents in this compartment. However, this increase was much higher in the PPV-susceptible plants than in the resistant apricot cultivar. Only in the PPV-susceptible apricot and peach plants was the increase in apoplastic H2O2 levels accompanied by an increase in electrolyte leakage. No changes in the electrolyte leakage were observed in the PPV-inoculated resistant apricot leaves, although a 42% increase in the apoplastic H2O2 levels was produced. Two-dimensional electrophoresis analyses revealed that the majority of the polypeptides in the apoplastic fluid had isoelectric points in the range of pI 4-6. The identification of proteins using MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and peptide mass fingerprinting analyses showed the induction of a thaumatin-like protein as well as the decrease of mandelonitrile lyase in peach apoplast due to PPV infection. However, most of the selected polypeptides showed no homology with known proteins. This fact emphasizes that, at least in Prunus, most of the functions of the apoplastic space remain unknown. We conclude that long-term PPV infection produced an oxidative stress in the leaf apoplast, contributing to the deleterious effects produced by PPV infection in leaves of inoculated, susceptible Prunus plants
publishDate 2008
dc.date.none.fl_str_mv 2008
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/4971
url http://hdl.handle.net/10261/4971
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Experimental Botany
vol 57:3813-3824
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 437751 bytes
application/pdf
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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