Autophagy and mitophagy in circulating immune cells of women with polycystic ovary syndrome: A cardiovascular perspective
Introduction: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder associated with systemic inflammation, oxidative stress, and increased cardiometabolic risk. While impaired autophagy and mitophagy have been implicated in tissue-specific dysfunction, their role in circulating immune cel...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO) |
| Repositorio: | r-FISABIO. Repositorio Institucional de Producción Científica |
| OAI Identifier: | oai:fisabio.fundanetsuite.com:p19401 |
| Acceso en línea: | https://fisabio.portalinvestigacion.com/publicaciones/19401 https://www.scopus.com/inward/record.uri?eid=2-s2.0-105017545148&doi=10.1016%2fj.freeradbiomed.2025.09.025&partnerID=40&md5=541c45d57b40d44bbe31a37dd77b9dc0 |
| Access Level: | acceso abierto |
| Palabra clave: | Autophagy Endothelial dysfunction Inflammation Mitophagy Oxidative stress PBMCs PCOS Adult Biomarkers Cardiovascular Diseases Case-Control Studies Cross-Sectional Studies Endothelial Cells Female Humans Leukocytes, Mononuclear Membrane Potential, Mitochondrial Neutrophils Oxidative Stress Peroxidase Polycystic Ovary Syndrome Young Adult beclin 1 C reactive protein cholesterol complement component C3 follitropin glutathione high density lipoprotein cholesterol low density lipoprotein cholesterol myeloperoxidase superoxide triacylglycerol tumor necrosis factor biological marker peroxidase adult anthropometry Article autophagy (cellular) cardiovascular parameters cardiovascular risk chemiluminescence immunoassay cholesterol blood level clinical article controlled study cross-sectional study diastolic blood pressure endothelial dysfunction female flow cytometry gene expression human immunocompetent cel |
| Sumario: | Introduction: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder associated with systemic inflammation, oxidative stress, and increased cardiometabolic risk. While impaired autophagy and mitophagy have been implicated in tissue-specific dysfunction, their role in circulating immune cells and relation to early vascular alterations remain unclear. Methods: In this cross-sectional study, 91 women (48 controls, 43 with PCOS) underwent anthropometric and biochemical assessment. Systemic markers included myeloperoxidase (MPO), glutathione, TNFa, and sP-selectin were determined in serum. Superoxide production (dHE), mitochondrial membrane potential (TMRM), and protein levels of BECLIN1, LC3II/I, P62, NBR1, and PINK1 were assessed in peripheral blood mononuclear cells (PBMCs). Neutrophil-endothelial cell interactions were analysed as subclinical markers of endothelial dysfunction. Results: PCOS patients presented hormonal, lipid, and glucose abnormalities, accompanied by redox imbalance, elevated MPO and superoxide levels, reduced glutathione, and increased mitochondrial membrane potential. Autophagy and mitophagy pathways were significantly impaired in PBMCs, and the expression of all assessed markers was reduced. These alterations were associated with androgen excess, oxidative stress, and inflammation. Elevated sP-selectin and enhanced neutrophil-endothelial interactions indicated early endothelial dysfunction in PCOS women. Conclusion: Our findings reveal that women with PCOS display autophagy and mitophagy impairment in immune cells, processes that are linked to oxidative and inflammatory stress and accompanied by endothelial alterations, highlighting a potential mechanistic pathway contributing to their cardiometabolic risk. © 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC license. http://creativecommons.org/licenses/by-nc/4.0/ |
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