Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus

[Data Availability Statement] The data supporting the reported results are in the Tripp laboratory at the University of Georgia.

Detalles Bibliográficos
Autores: Murray, Jackelyn, Nogales, Aitor, Martínez-Sobrido, Luis, Martin, David E., Sancilio, Fred D., Tripp, Ralph A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/382530
Acceso en línea:http://hdl.handle.net/10261/382530
Access Level:acceso abierto
Palabra clave:Influenza virus
Probenecid
Oseltamivir
NHBE cells
Antiviral
Plaque assay
Fluorescent focus assay
id ES_4ff74afc8d1cc202f92b64de8531a18f
oai_identifier_str oai:digital.csic.es:10261/382530
network_acronym_str ES
network_name_str España
repository_id_str
spelling Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter VirusMurray, JackelynNogales, AitorMartínez-Sobrido, LuisMartin, David E.Sancilio, Fred D.Tripp, Ralph A.Influenza virusProbenecidOseltamivirNHBE cellsAntiviralPlaque assayFluorescent focus assay[Data Availability Statement] The data supporting the reported results are in the Tripp laboratory at the University of Georgia.It is essential to understand the molecular mechanisms of influenza antiviral therapeutics to evaluate their efficacy. Virus plaque assays are commonly used to assess the antiviral effects of drugs on virus replication; however, this method is labor-intensive and can present challenges. We avoided this method by using a replication-competent influenza A virus (IAV) expressing a reporter fluorescent gene fused to the non-structural protein 1 (NS1) gene. The reporter IAV was detectable in normal human bronchoepithelial (NHBE) infected cells and offered an improved method to determine the therapeutic efficacy of the antiviral drugs probenecid and oseltamivir compared to a standard plaque assay. This method provides an excellent means for evaluating therapeutic approaches against IAV.An endowment from the Georgia Research Alliance provided financial support for Ralph Tripp at the University of Georgia for this research and the preparation of this article.Peer reviewedMultidisciplinary Digital Publishing InstituteUniversity of GeorgiaNogales, Aitor [0000-0002-2424-7900]Martínez-Sobrido, Luis [0000-0001-7084-0804]Martin, David E. [0000-0003-2820-5983]Tripp, Ralph A. [0000-0002-2924-9956]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/382530reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/v17030335Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3825302026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
title Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
spellingShingle Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
Murray, Jackelyn
Influenza virus
Probenecid
Oseltamivir
NHBE cells
Antiviral
Plaque assay
Fluorescent focus assay
title_short Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
title_full Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
title_fullStr Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
title_full_unstemmed Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
title_sort Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus
dc.creator.none.fl_str_mv Murray, Jackelyn
Nogales, Aitor
Martínez-Sobrido, Luis
Martin, David E.
Sancilio, Fred D.
Tripp, Ralph A.
author Murray, Jackelyn
author_facet Murray, Jackelyn
Nogales, Aitor
Martínez-Sobrido, Luis
Martin, David E.
Sancilio, Fred D.
Tripp, Ralph A.
author_role author
author2 Nogales, Aitor
Martínez-Sobrido, Luis
Martin, David E.
Sancilio, Fred D.
Tripp, Ralph A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv University of Georgia
Nogales, Aitor [0000-0002-2424-7900]
Martínez-Sobrido, Luis [0000-0001-7084-0804]
Martin, David E. [0000-0003-2820-5983]
Tripp, Ralph A. [0000-0002-2924-9956]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Influenza virus
Probenecid
Oseltamivir
NHBE cells
Antiviral
Plaque assay
Fluorescent focus assay
topic Influenza virus
Probenecid
Oseltamivir
NHBE cells
Antiviral
Plaque assay
Fluorescent focus assay
description [Data Availability Statement] The data supporting the reported results are in the Tripp laboratory at the University of Georgia.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/382530
url http://hdl.handle.net/10261/382530
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/v17030335
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407852479643648
score 15,811543

Similares en LA Referencia