Pericardium decellularization in a one-day, two-step protocol

Scaffolds used in tissue engineering can be obtained from synthetic or natural materials, always focusing the effort on mimicking the extracellular matrix of human native tissue. In this study, a decellularization process is used to obtain an acellular, biocompatible non-cytotoxic human pericardium...

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Autores: López-Chicón, P, Martínez, JIR, Castells-Sala, C, Lopez-Puerto, L, Ruiz-Ponsell, L, Fariñas, O, Vilarrodona, A
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p18217
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18217
Access Level:acceso abierto
Palabra clave:Decellularization
Pericardial allograft
Human acellular matrix
Tissue establishment
Tissue engineering
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spelling Pericardium decellularization in a one-day, two-step protocolLópez-Chicón, PMartínez, JIRCastells-Sala, CLopez-Puerto, LRuiz-Ponsell, LFariñas, OVilarrodona, ADecellularizationPericardial allograftHuman acellular matrixTissue establishmentTissue engineeringScaffolds used in tissue engineering can be obtained from synthetic or natural materials, always focusing the effort on mimicking the extracellular matrix of human native tissue. In this study, a decellularization process is used to obtain an acellular, biocompatible non-cytotoxic human pericardium graft as a bio-substitute. An enzymatic and hypertonic method was used to decellularize the pericardium. Histological analyses were performed to determine the absence of cells and ensure the integrity of the extracellular matrix (ECM). In order to measure the effect of the decellularization process on the tissue's biological and mechanical properties, residual genetic content and ECM biomolecules (collagen, elastin, and glycosaminoglycan) were quantified and the tissue's tensile strength was tested. Preservation of the biomolecules, a residual genetic content below 50 ng/mg dry tissue, and maintenance of the histological structure provided evidence for the efficacy of the decellularization process, while preserving the ECM. Moreover, the acellular tissue retains its mechanical properties, as shown by the biomechanical tests. Our group has shown that the acellular pericardial matrix obtained through the super-fast decellularization protocol developed recently retains the desired biomechanical and structural properties, suggesting that it is suitable for a broad range of clinical indications.SPRINGER2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18217MOLECULAR AND CELLULAR BIOCHEMISTRYISSN: 03008177ISSNe: 15734919reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p182172026-06-14T12:41:47Z
dc.title.none.fl_str_mv Pericardium decellularization in a one-day, two-step protocol
title Pericardium decellularization in a one-day, two-step protocol
spellingShingle Pericardium decellularization in a one-day, two-step protocol
López-Chicón, P
Decellularization
Pericardial allograft
Human acellular matrix
Tissue establishment
Tissue engineering
title_short Pericardium decellularization in a one-day, two-step protocol
title_full Pericardium decellularization in a one-day, two-step protocol
title_fullStr Pericardium decellularization in a one-day, two-step protocol
title_full_unstemmed Pericardium decellularization in a one-day, two-step protocol
title_sort Pericardium decellularization in a one-day, two-step protocol
dc.creator.none.fl_str_mv López-Chicón, P
Martínez, JIR
Castells-Sala, C
Lopez-Puerto, L
Ruiz-Ponsell, L
Fariñas, O
Vilarrodona, A
author López-Chicón, P
author_facet López-Chicón, P
Martínez, JIR
Castells-Sala, C
Lopez-Puerto, L
Ruiz-Ponsell, L
Fariñas, O
Vilarrodona, A
author_role author
author2 Martínez, JIR
Castells-Sala, C
Lopez-Puerto, L
Ruiz-Ponsell, L
Fariñas, O
Vilarrodona, A
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Decellularization
Pericardial allograft
Human acellular matrix
Tissue establishment
Tissue engineering
topic Decellularization
Pericardial allograft
Human acellular matrix
Tissue establishment
Tissue engineering
description Scaffolds used in tissue engineering can be obtained from synthetic or natural materials, always focusing the effort on mimicking the extracellular matrix of human native tissue. In this study, a decellularization process is used to obtain an acellular, biocompatible non-cytotoxic human pericardium graft as a bio-substitute. An enzymatic and hypertonic method was used to decellularize the pericardium. Histological analyses were performed to determine the absence of cells and ensure the integrity of the extracellular matrix (ECM). In order to measure the effect of the decellularization process on the tissue's biological and mechanical properties, residual genetic content and ECM biomolecules (collagen, elastin, and glycosaminoglycan) were quantified and the tissue's tensile strength was tested. Preservation of the biomolecules, a residual genetic content below 50 ng/mg dry tissue, and maintenance of the histological structure provided evidence for the efficacy of the decellularization process, while preserving the ECM. Moreover, the acellular tissue retains its mechanical properties, as shown by the biomechanical tests. Our group has shown that the acellular pericardial matrix obtained through the super-fast decellularization protocol developed recently retains the desired biomechanical and structural properties, suggesting that it is suitable for a broad range of clinical indications.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18217
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18217
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv SPRINGER
publisher.none.fl_str_mv SPRINGER
dc.source.none.fl_str_mv MOLECULAR AND CELLULAR BIOCHEMISTRY
ISSN: 03008177
ISSNe: 15734919
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
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