Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages

The role of 2-hydroxy-(4-methylseleno)butanoic acid (OH-SeMet), a form of organic selenium (Se), in selenoprotein synthesis and inflammatory response of THP1-derived macrophages stimulated with lipopolysaccharide (LPS) has been investigated. Glutathione peroxidase (GPX) activity, GPX1 gene expressio...

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Autores: Campo Sabariz, Joan, García-Vara, Adriana, Moral Anter, David, Briens, M., Hachemi, Mohammed A., Pinloche, Eric, Ferrer i Roig, Ruth, Martín Venegas, Raquel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/192208
Acceso en línea:https://hdl.handle.net/2445/192208
Access Level:acceso abierto
Palabra clave:Estrès oxidatiu
Intestins
Seleni
Oxidative stress
Intestines
Selenium
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spelling Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated MacrophagesCampo Sabariz, JoanGarcía-Vara, AdrianaMoral Anter, DavidBriens, M.Hachemi, Mohammed A.Pinloche, EricFerrer i Roig, RuthMartín Venegas, RaquelEstrès oxidatiuIntestinsSeleniOxidative stressIntestinesSeleniumThe role of 2-hydroxy-(4-methylseleno)butanoic acid (OH-SeMet), a form of organic selenium (Se), in selenoprotein synthesis and inflammatory response of THP1-derived macrophages stimulated with lipopolysaccharide (LPS) has been investigated. Glutathione peroxidase (GPX) activity, GPX1 gene expression, selenoprotein P (SELENOP) protein and gene expression, and reactive oxygen species (ROS) production were studied in Se-deprived conditions (6 and 24 h). Then, macrophages were supplemented with OH-SeMet for 72 h and GPX1 and SELENOP gene expression were determined. The protective effect of OH-SeMet against oxidative stress was studied in H2O2-stimulated macrophages, as well as the effect on GPX1 gene expression, oxidative stress, cytokine production (TNFα, IL-1β and IL-10), and phagocytic and killing capacities after LPS stimulation. Se deprivation induced a reduction in GPX activity, GPX1 gene expression, and SELENOP protein and gene expression at 24 h. OH-SeMet upregulated GPX1 and SELENOP gene expression and decreased ROS production after H2O2 treatment. In LPS-stimulated macrophages, OH-SeMet upregulated GPX1 gene expression, enhanced phagocytic and killing capacities, and reduced ROS and cytokine production. Therefore, OH-SeMet supplementation supports selenoprotein expression and controls oxidative burst and cytokine production while enhancing phagocytic and killing capacities, modulating the inflammatory response, and avoiding the potentially toxic insult produced by highly activated macrophages. Keywords: cytokine production; glutathione peroxidase; immune response; macrophage polarization; oxidative stress; phagocytosis; selenium deprivation; selenoprotein P; 2-hydroxy-(4-methylseleno)butanoic acidMDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/192208Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/antiox11101876Antioxidants, 2022, vol. 11, p. 1876https://doi.org/10.3390/antiox11101876cc-by (c) Campo Sabariz, Joan et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1922082026-05-27T06:46:51Z
dc.title.none.fl_str_mv Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
title Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
spellingShingle Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
Campo Sabariz, Joan
Estrès oxidatiu
Intestins
Seleni
Oxidative stress
Intestines
Selenium
title_short Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
title_full Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
title_fullStr Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
title_full_unstemmed Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
title_sort Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages
dc.creator.none.fl_str_mv Campo Sabariz, Joan
García-Vara, Adriana
Moral Anter, David
Briens, M.
Hachemi, Mohammed A.
Pinloche, Eric
Ferrer i Roig, Ruth
Martín Venegas, Raquel
author Campo Sabariz, Joan
author_facet Campo Sabariz, Joan
García-Vara, Adriana
Moral Anter, David
Briens, M.
Hachemi, Mohammed A.
Pinloche, Eric
Ferrer i Roig, Ruth
Martín Venegas, Raquel
author_role author
author2 García-Vara, Adriana
Moral Anter, David
Briens, M.
Hachemi, Mohammed A.
Pinloche, Eric
Ferrer i Roig, Ruth
Martín Venegas, Raquel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estrès oxidatiu
Intestins
Seleni
Oxidative stress
Intestines
Selenium
topic Estrès oxidatiu
Intestins
Seleni
Oxidative stress
Intestines
Selenium
description The role of 2-hydroxy-(4-methylseleno)butanoic acid (OH-SeMet), a form of organic selenium (Se), in selenoprotein synthesis and inflammatory response of THP1-derived macrophages stimulated with lipopolysaccharide (LPS) has been investigated. Glutathione peroxidase (GPX) activity, GPX1 gene expression, selenoprotein P (SELENOP) protein and gene expression, and reactive oxygen species (ROS) production were studied in Se-deprived conditions (6 and 24 h). Then, macrophages were supplemented with OH-SeMet for 72 h and GPX1 and SELENOP gene expression were determined. The protective effect of OH-SeMet against oxidative stress was studied in H2O2-stimulated macrophages, as well as the effect on GPX1 gene expression, oxidative stress, cytokine production (TNFα, IL-1β and IL-10), and phagocytic and killing capacities after LPS stimulation. Se deprivation induced a reduction in GPX activity, GPX1 gene expression, and SELENOP protein and gene expression at 24 h. OH-SeMet upregulated GPX1 and SELENOP gene expression and decreased ROS production after H2O2 treatment. In LPS-stimulated macrophages, OH-SeMet upregulated GPX1 gene expression, enhanced phagocytic and killing capacities, and reduced ROS and cytokine production. Therefore, OH-SeMet supplementation supports selenoprotein expression and controls oxidative burst and cytokine production while enhancing phagocytic and killing capacities, modulating the inflammatory response, and avoiding the potentially toxic insult produced by highly activated macrophages. Keywords: cytokine production; glutathione peroxidase; immune response; macrophage polarization; oxidative stress; phagocytosis; selenium deprivation; selenoprotein P; 2-hydroxy-(4-methylseleno)butanoic acid
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/192208
url https://hdl.handle.net/2445/192208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/antiox11101876
Antioxidants, 2022, vol. 11, p. 1876
https://doi.org/10.3390/antiox11101876
dc.rights.none.fl_str_mv cc-by (c) Campo Sabariz, Joan et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Campo Sabariz, Joan et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407831647584256
score 15,300719

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