Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms

Aggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as bioflms, are associated with several infectious diseases. One of the main components of these bioflms is alginate, a homo- and hetero-polysaccharide that consists of β-D...

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Autores: Blanco-Cabra, Núria, Paetzold, Bernhard, Ferrar, Tony, Mazzolini, Rocco, Torrents Serra, Eduard, Serrano, Luis, LLuch-Senar, Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/177223
Acceso en línea:https://hdl.handle.net/2445/177223
Access Level:acceso abierto
Palabra clave:Pseudomonas
Sistema immunitari
Biofilms
Immune system
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spelling Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilmsBlanco-Cabra, NúriaPaetzold, BernhardFerrar, TonyMazzolini, RoccoTorrents Serra, EduardSerrano, LuisLLuch-Senar, MariaPseudomonasSistema immunitariBiofilmsPseudomonasImmune systemBiofilmsAggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as bioflms, are associated with several infectious diseases. One of the main components of these bioflms is alginate, a homo- and hetero-polysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G) units. Alginate lyases degrade this sugar and have been proposed as biotherapeutic agents to dissolve P. aeruginosa bioflms. However, there are contradictory reports in the literature regarding the efcacy of alginate lyases against bioflms and their synergistic efect with antibiotics. We found that most positive reports used a commercial crude extract from Flavobacterium multivorum as the alginate lyase source. By using anion exchange chromatography coupled to nano LC MS/MS, we identifed two distinct enzymes in this extract, one has both polyM and polyG (polyM/G) degradation activities and it is similar in sequence to a broad-spectrum alginate lyase from Flavobacterium sp. S20 (Alg2A). The other enzyme has only polyG activity and it is similar in sequence to AlyA1 from Zobellia galactanivorans. By characterizing both of these enzymes together with three recombinant alginate lyases (a polyM, a polyG and a polyM/G), we showed that only enzymes with polyM/G activity such as Alg2A and A1-II' (alginate lyase from Sphingomonas sp.) are efective in dissolving bioflms. Furthermore, both activities are required to have a synergistic efect with antibiotics.Nature Publishing Group2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/177223Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/s41598-020-66293-2Scientific Reports, 2020, vol. 10, num. 1, p. 9390https://doi.org/10.1038/s41598-020-66293-2info:eu-repo/grantAgreement/EC/H2020/670216cc-by (c) Blanco Cabra, Nuria et al., 2020https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1772232026-05-27T06:46:51Z
dc.title.none.fl_str_mv Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
title Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
spellingShingle Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
Blanco-Cabra, Núria
Pseudomonas
Sistema immunitari
Biofilms
Pseudomonas
Immune system
Biofilms
title_short Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
title_full Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
title_fullStr Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
title_full_unstemmed Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
title_sort Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
dc.creator.none.fl_str_mv Blanco-Cabra, Núria
Paetzold, Bernhard
Ferrar, Tony
Mazzolini, Rocco
Torrents Serra, Eduard
Serrano, Luis
LLuch-Senar, Maria
author Blanco-Cabra, Núria
author_facet Blanco-Cabra, Núria
Paetzold, Bernhard
Ferrar, Tony
Mazzolini, Rocco
Torrents Serra, Eduard
Serrano, Luis
LLuch-Senar, Maria
author_role author
author2 Paetzold, Bernhard
Ferrar, Tony
Mazzolini, Rocco
Torrents Serra, Eduard
Serrano, Luis
LLuch-Senar, Maria
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Pseudomonas
Sistema immunitari
Biofilms
Pseudomonas
Immune system
Biofilms
topic Pseudomonas
Sistema immunitari
Biofilms
Pseudomonas
Immune system
Biofilms
description Aggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as bioflms, are associated with several infectious diseases. One of the main components of these bioflms is alginate, a homo- and hetero-polysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G) units. Alginate lyases degrade this sugar and have been proposed as biotherapeutic agents to dissolve P. aeruginosa bioflms. However, there are contradictory reports in the literature regarding the efcacy of alginate lyases against bioflms and their synergistic efect with antibiotics. We found that most positive reports used a commercial crude extract from Flavobacterium multivorum as the alginate lyase source. By using anion exchange chromatography coupled to nano LC MS/MS, we identifed two distinct enzymes in this extract, one has both polyM and polyG (polyM/G) degradation activities and it is similar in sequence to a broad-spectrum alginate lyase from Flavobacterium sp. S20 (Alg2A). The other enzyme has only polyG activity and it is similar in sequence to AlyA1 from Zobellia galactanivorans. By characterizing both of these enzymes together with three recombinant alginate lyases (a polyM, a polyG and a polyM/G), we showed that only enzymes with polyM/G activity such as Alg2A and A1-II' (alginate lyase from Sphingomonas sp.) are efective in dissolving bioflms. Furthermore, both activities are required to have a synergistic efect with antibiotics.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177223
url https://hdl.handle.net/2445/177223
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-66293-2
Scientific Reports, 2020, vol. 10, num. 1, p. 9390
https://doi.org/10.1038/s41598-020-66293-2
info:eu-repo/grantAgreement/EC/H2020/670216
dc.rights.none.fl_str_mv cc-by (c) Blanco Cabra, Nuria et al., 2020
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Blanco Cabra, Nuria et al., 2020
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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