Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency

The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional group nature on surfactant DNA compacting efficiency. The surfactants inve...

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Autores: Lebrón Romero, José Antonio, López-Cornejo, María del Pilar, García Dionisio, Elena, Huertas Sánchez, Pablo, García Calderón, Margarita, Moyá Morán, María Luisa, Ostos Marcos, Francisco José, López López, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/115384
Acceso en línea:https://hdl.handle.net/11441/115384
https://doi.org/10.3390/pharmaceutics13040589
Access Level:acceso abierto
Palabra clave:Cationic surfactants
DNA compacting efficiency
Liposomes
Lipoplexes
Gene transfection
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spelling Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting EfficiencyLebrón Romero, José AntonioLópez-Cornejo, María del PilarGarcía Dionisio, ElenaHuertas Sánchez, PabloGarcía Calderón, MargaritaMoyá Morán, María LuisaOstos Marcos, Francisco JoséLópez López, ManuelCationic surfactantsDNA compacting efficiencyLiposomesLipoplexesGene transfectionThe interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional group nature on surfactant DNA compacting efficiency. The surfactants investigated were dodecyltriethylammonium bromide (DTEABr), triethyl(1-phenoxydodecyl)ammonium bromide (12PhBr), triethyl(2-naphthoxydodecyl)ammonium bromide (12NBr) and 11-(isonicotinoyloxy)-N,N,N-triethyl-1-undecanaminium bromide (11PyBr). Results made evident that the surfactants’ tendencies to self-aggregation is the key factor determining their efficiency to compact the nucleic acid. Subsequently, DOPE/12NBr/pEGFP-C1 lipoplexes, with different cationic surfactant molar fractions (α) and mass ratios (L/D), were prepared and characterized. DOPE is a zwitterionic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, and the plasmid pEGFP-C1 carries a GFP coding sequence with the necessary regulatory elements for constitutive expression of the gene in human cells. 12NBr was chosen because it was the most efficient DNA compacting agent among the surfactants investigated. Finally, the cytotoxicity and transfection efficiency (TE) of DOPE/12NBr/pDNA lipoplexes, with different compositions, were investigated.Junta de Andalucía FQM-206, FQM-274, PI-0005-2018Universidad de Sevilla PP2019/00000748Premio Mensual Publicación Científica Destacada de la US. Facultad de QuímicaMultidisciplinary Digital Publishing Institute (MDPI)Química FísicaGenéticaBioquímica Vegetal y Biología Molecular2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/115384https://doi.org/10.3390/pharmaceutics13040589reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPharmaceutics, 13 (4), 589.FQM-206FQM-274PI-0005-2018PP2019/00000748https://doi.org/10.3390/pharmaceutics13040589info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1153842026-06-17T12:51:07Z
dc.title.none.fl_str_mv Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
title Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
spellingShingle Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
Lebrón Romero, José Antonio
Cationic surfactants
DNA compacting efficiency
Liposomes
Lipoplexes
Gene transfection
title_short Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
title_full Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
title_fullStr Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
title_full_unstemmed Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
title_sort Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
dc.creator.none.fl_str_mv Lebrón Romero, José Antonio
López-Cornejo, María del Pilar
García Dionisio, Elena
Huertas Sánchez, Pablo
García Calderón, Margarita
Moyá Morán, María Luisa
Ostos Marcos, Francisco José
López López, Manuel
author Lebrón Romero, José Antonio
author_facet Lebrón Romero, José Antonio
López-Cornejo, María del Pilar
García Dionisio, Elena
Huertas Sánchez, Pablo
García Calderón, Margarita
Moyá Morán, María Luisa
Ostos Marcos, Francisco José
López López, Manuel
author_role author
author2 López-Cornejo, María del Pilar
García Dionisio, Elena
Huertas Sánchez, Pablo
García Calderón, Margarita
Moyá Morán, María Luisa
Ostos Marcos, Francisco José
López López, Manuel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Química Física
Genética
Bioquímica Vegetal y Biología Molecular
dc.subject.none.fl_str_mv Cationic surfactants
DNA compacting efficiency
Liposomes
Lipoplexes
Gene transfection
topic Cationic surfactants
DNA compacting efficiency
Liposomes
Lipoplexes
Gene transfection
description The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional group nature on surfactant DNA compacting efficiency. The surfactants investigated were dodecyltriethylammonium bromide (DTEABr), triethyl(1-phenoxydodecyl)ammonium bromide (12PhBr), triethyl(2-naphthoxydodecyl)ammonium bromide (12NBr) and 11-(isonicotinoyloxy)-N,N,N-triethyl-1-undecanaminium bromide (11PyBr). Results made evident that the surfactants’ tendencies to self-aggregation is the key factor determining their efficiency to compact the nucleic acid. Subsequently, DOPE/12NBr/pEGFP-C1 lipoplexes, with different cationic surfactant molar fractions (α) and mass ratios (L/D), were prepared and characterized. DOPE is a zwitterionic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, and the plasmid pEGFP-C1 carries a GFP coding sequence with the necessary regulatory elements for constitutive expression of the gene in human cells. 12NBr was chosen because it was the most efficient DNA compacting agent among the surfactants investigated. Finally, the cytotoxicity and transfection efficiency (TE) of DOPE/12NBr/pDNA lipoplexes, with different compositions, were investigated.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/115384
https://doi.org/10.3390/pharmaceutics13040589
url https://hdl.handle.net/11441/115384
https://doi.org/10.3390/pharmaceutics13040589
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Pharmaceutics, 13 (4), 589.
FQM-206
FQM-274
PI-0005-2018
PP2019/00000748
https://doi.org/10.3390/pharmaceutics13040589
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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