Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)

Recombinant gonadotropins—synthetic versions of pituitary hormones—are increasingly utilised in aquaculture for broodstock management. In this study, we explored how recombinant follicle-stimulating hormone (rFsh) affects spermatogenesis in meagre (Argyrosomus regius), with the aim of enhancing our...

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Autores: Ventriglia, Gianluca, Duncan, Neil, Giménez, Ignacio, Mylonas, Constantinos C., Pousis, Chrysovalentinos, Varvara, Caterina, Valentini, Luisa, Passantino, Letizia, Corriero, Aldo, Zupa, Rosa
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:dnet:irtapubpro__::13ee74957c16be5dd84687b375ba9e9e
Acceso en línea:http://hdl.handle.net/20.500.12327/4925
https://doi.org/10.3390/ani15182668
Access Level:acceso abierto
Palabra clave:637
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spelling Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)Ventriglia, GianlucaDuncan, NeilGiménez, IgnacioMylonas, Constantinos C.Pousis, ChrysovalentinosVarvara, CaterinaValentini, LuisaPassantino, LetiziaCorriero, AldoZupa, Rosa637Recombinant gonadotropins—synthetic versions of pituitary hormones—are increasingly utilised in aquaculture for broodstock management. In this study, we explored how recombinant follicle-stimulating hormone (rFsh) affects spermatogenesis in meagre (Argyrosomus regius), with the aim of enhancing our understanding of testicular apoptosis—a common form of programmed cell death involved in modulating germ cell development and sperm output. We assessed germ cell composition and testicular apoptosis in both pre-pubertal meagre treated with rFsh and adult fish in two spermatogenic phases (proliferative and meiotic). In pre-pubertal fish, rFsh treatment led to larger testes, wider seminiferous tubules, and increased sperm presence. There was a reduction in early spermatogonia (undifferentiated germ cells) but a rise in dividing germ cells, indicating progression toward meiosis. Notably, treated fish exhibited significantly lower spermatogonial apoptosis compared to controls, suggesting rFsh promotes germ cell survival by mitigating natural cell death. In adult fish, the density of spermatogonia decreased with the progression of spermatogenesis, whereas apoptosis increased and often involved entire clones of germ cells. Overall, the study demonstrates that rFsh effectively induces testicular maturation in pre-pubertal meagre by promoting progression toward meiosis and highlights how the role of apoptosis varies across reproductive stages.The present work was funded by the project NewTechAqua (European Union’s Programme H2020, GA 862658) awarded to N.D., C.C.M., and A.C., and by a grant from the Apulian Region to R.Z. (POR PUGLIA FESR-FSE 2014/2020—Asse X-Azione 10.4—Research for Innovation—REFIN).info:eu-repo/semantics/publishedVersionMDPIProducció AnimalAqüicultura202520252025info:eu-repo/semantics/article13application/pdfhttp://hdl.handle.net/20.500.12327/4925https://doi.org/10.3390/ani15182668reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésAnimalsEC/H2020/862658/EU/New Technologies, Tools and Strategies for a Sustainable, Resilient and Innovative European Aquaculture/NewTechAquaAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:irtapubpro__::13ee74957c16be5dd84687b375ba9e9e2026-06-16T08:51:17Z
dc.title.none.fl_str_mv Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
title Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
spellingShingle Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
Ventriglia, Gianluca
637
title_short Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
title_full Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
title_fullStr Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
title_full_unstemmed Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
title_sort Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (Argyrosomus regius)
dc.creator.none.fl_str_mv Ventriglia, Gianluca
Duncan, Neil
Giménez, Ignacio
Mylonas, Constantinos C.
Pousis, Chrysovalentinos
Varvara, Caterina
Valentini, Luisa
Passantino, Letizia
Corriero, Aldo
Zupa, Rosa
author Ventriglia, Gianluca
author_facet Ventriglia, Gianluca
Duncan, Neil
Giménez, Ignacio
Mylonas, Constantinos C.
Pousis, Chrysovalentinos
Varvara, Caterina
Valentini, Luisa
Passantino, Letizia
Corriero, Aldo
Zupa, Rosa
author_role author
author2 Duncan, Neil
Giménez, Ignacio
Mylonas, Constantinos C.
Pousis, Chrysovalentinos
Varvara, Caterina
Valentini, Luisa
Passantino, Letizia
Corriero, Aldo
Zupa, Rosa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
dc.subject.none.fl_str_mv 637
topic 637
description Recombinant gonadotropins—synthetic versions of pituitary hormones—are increasingly utilised in aquaculture for broodstock management. In this study, we explored how recombinant follicle-stimulating hormone (rFsh) affects spermatogenesis in meagre (Argyrosomus regius), with the aim of enhancing our understanding of testicular apoptosis—a common form of programmed cell death involved in modulating germ cell development and sperm output. We assessed germ cell composition and testicular apoptosis in both pre-pubertal meagre treated with rFsh and adult fish in two spermatogenic phases (proliferative and meiotic). In pre-pubertal fish, rFsh treatment led to larger testes, wider seminiferous tubules, and increased sperm presence. There was a reduction in early spermatogonia (undifferentiated germ cells) but a rise in dividing germ cells, indicating progression toward meiosis. Notably, treated fish exhibited significantly lower spermatogonial apoptosis compared to controls, suggesting rFsh promotes germ cell survival by mitigating natural cell death. In adult fish, the density of spermatogonia decreased with the progression of spermatogenesis, whereas apoptosis increased and often involved entire clones of germ cells. Overall, the study demonstrates that rFsh effectively induces testicular maturation in pre-pubertal meagre by promoting progression toward meiosis and highlights how the role of apoptosis varies across reproductive stages.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/4925
https://doi.org/10.3390/ani15182668
url http://hdl.handle.net/20.500.12327/4925
https://doi.org/10.3390/ani15182668
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Animals
EC/H2020/862658/EU/New Technologies, Tools and Strategies for a Sustainable, Resilient and Innovative European Aquaculture/NewTechAqua
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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