A bump-hole strategy for increased stringency of cell-specific metabolic labeling of RNA
Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Here...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/109478 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/109478 |
| Access Level: | acceso abierto |
| Palabra clave: | 577.15 577.2 575 Genetics Imaging probes Labeling Peptides and proteins Uracil Bioquímica (Biología) Biología molecular (Biología) Genética 2403 Bioquímica 2415 Biología Molecular 2302.09 Enzimología 2302.04 Genética Bioquímica |
| Sumario: | Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency. |
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