MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma

MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+...

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Autores: Sandoval, Juan, Diaz-Lagares, Angel, Salgado, Rocío, Servitje Bedate, Octavio, Climent, Fina, Ortiz Romero, Pablo Luis, Pérez-Ferriols, Amparo, Garcia-Muret, Maria P., Estrach Panella, Ma. Teresa (María Teresa), Garcia, Mar, Nonell, Lara, Esteller, Manel, Pujol, Ramon M., Espinet Solà, Blanca, Gallardo, F. (Fernando)
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/111029
Acceso en línea:https://hdl.handle.net/2445/111029
Access Level:acceso abierto
Palabra clave:Micro RNAs
ADN
Metilació
Limfomes
Cèl·lules T
MicroRNAs
DNA
Methylation
Lymphomas
T cells
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spelling MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphomaSandoval, JuanDiaz-Lagares, AngelSalgado, RocíoServitje Bedate, OctavioCliment, FinaOrtiz Romero, Pablo LuisPérez-Ferriols, AmparoGarcia-Muret, Maria P.Estrach Panella, Ma. Teresa (María Teresa)Garcia, MarNonell, LaraEsteller, ManelPujol, Ramon M.Espinet Solà, BlancaGallardo, F. (Fernando)Micro RNAsADNMetilacióLimfomesCèl·lules TMicroRNAsDNAMethylationLymphomasT cellsMicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients.Society for Investigative Dermatology2017201720152017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion10 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/111029Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1038/jid.2014.487Journal of Investigative Dermatology, 2015, vol. 135, num. 4, p. 1128-1137https://doi.org/10.1038/jid.2014.487(c) Sandoval, Juan et al., 2015info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1110292026-05-29T05:05:01Z
dc.title.none.fl_str_mv MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
title MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
spellingShingle MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
Sandoval, Juan
Micro RNAs
ADN
Metilació
Limfomes
Cèl·lules T
MicroRNAs
DNA
Methylation
Lymphomas
T cells
title_short MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
title_full MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
title_fullStr MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
title_full_unstemmed MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
title_sort MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma
dc.creator.none.fl_str_mv Sandoval, Juan
Diaz-Lagares, Angel
Salgado, Rocío
Servitje Bedate, Octavio
Climent, Fina
Ortiz Romero, Pablo Luis
Pérez-Ferriols, Amparo
Garcia-Muret, Maria P.
Estrach Panella, Ma. Teresa (María Teresa)
Garcia, Mar
Nonell, Lara
Esteller, Manel
Pujol, Ramon M.
Espinet Solà, Blanca
Gallardo, F. (Fernando)
author Sandoval, Juan
author_facet Sandoval, Juan
Diaz-Lagares, Angel
Salgado, Rocío
Servitje Bedate, Octavio
Climent, Fina
Ortiz Romero, Pablo Luis
Pérez-Ferriols, Amparo
Garcia-Muret, Maria P.
Estrach Panella, Ma. Teresa (María Teresa)
Garcia, Mar
Nonell, Lara
Esteller, Manel
Pujol, Ramon M.
Espinet Solà, Blanca
Gallardo, F. (Fernando)
author_role author
author2 Diaz-Lagares, Angel
Salgado, Rocío
Servitje Bedate, Octavio
Climent, Fina
Ortiz Romero, Pablo Luis
Pérez-Ferriols, Amparo
Garcia-Muret, Maria P.
Estrach Panella, Ma. Teresa (María Teresa)
Garcia, Mar
Nonell, Lara
Esteller, Manel
Pujol, Ramon M.
Espinet Solà, Blanca
Gallardo, F. (Fernando)
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Micro RNAs
ADN
Metilació
Limfomes
Cèl·lules T
MicroRNAs
DNA
Methylation
Lymphomas
T cells
topic Micro RNAs
ADN
Metilació
Limfomes
Cèl·lules T
MicroRNAs
DNA
Methylation
Lymphomas
T cells
description MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/111029
url https://hdl.handle.net/2445/111029
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1038/jid.2014.487
Journal of Investigative Dermatology, 2015, vol. 135, num. 4, p. 1128-1137
https://doi.org/10.1038/jid.2014.487
dc.rights.none.fl_str_mv (c) Sandoval, Juan et al., 2015
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Sandoval, Juan et al., 2015
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Investigative Dermatology
publisher.none.fl_str_mv Society for Investigative Dermatology
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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