Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity

The aim of this research was to investigate differential gene expression of cyclin-dependent kinase inhibitors (CKIs) in white adipose tissue (WAT) and liver from high-fat fed male Wistar rats with or without vitamin C (VC) supplementation (750 mg/kg of body weight). After 56 d of experimentation, a...

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Autores: Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b, Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c, Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb, Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82, Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Formato: artículo
Fecha de publicación:2009
País:España
Recursos:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/28191
Acesso em linha:https://hdl.handle.net/10171/28191
Access Level:acceso abierto
Palavra-chave:Cyclin-dependent kinase inhibitor
Cafeteria diet
Ascorbic acid
Adipose tissue
Fatty liver
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network_name_str España
repository_id_str
spelling Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesityBoque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989bCampión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817cMilagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebMoreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131Cyclin-dependent kinase inhibitorCafeteria dietAscorbic acidAdipose tissueFatty liverThe aim of this research was to investigate differential gene expression of cyclin-dependent kinase inhibitors (CKIs) in white adipose tissue (WAT) and liver from high-fat fed male Wistar rats with or without vitamin C (VC) supplementation (750 mg/kg of body weight). After 56 d of experimentation, animals fed on a cafeteria diet increased significantly body weights and total body fat. Reverse transcription-polymerase chain reaction (RT-PCR) studies showed that cafeteria diet decreased p21 and p57 mRNA expression in subcutaneous WAT and increased p21 mRNA in liver. Overall, these data provide new information about the role of high fat intake on mRNA levels of several CKIs with implications in adipogenesis, cell metabolism and weight homeostasis. Interestingly, VC supplementation partially prevented diet-induced adiposity and increased p27 mRNA in liver without any changes in the other tissues and genes analyzed. Thus, hepatic mRNA changes induced by ascorbic acid indicate a possible role of these genes in diet-induced oxidative stress processes.Pharmaceutical Society of JapanDadun. Depósito Académico Digital Universidad de Navarra20132013-03-2920092009-01-0120092009-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/28191reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/281912026-06-21T12:47:57Z
dc.title.none.fl_str_mv Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
title Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
spellingShingle Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b
Cyclin-dependent kinase inhibitor
Cafeteria diet
Ascorbic acid
Adipose tissue
Fatty liver
title_short Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
title_full Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
title_fullStr Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
title_full_unstemmed Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
title_sort Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
dc.creator.none.fl_str_mv Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b
author_facet Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author_role author
author2 Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Cyclin-dependent kinase inhibitor
Cafeteria diet
Ascorbic acid
Adipose tissue
Fatty liver
topic Cyclin-dependent kinase inhibitor
Cafeteria diet
Ascorbic acid
Adipose tissue
Fatty liver
description The aim of this research was to investigate differential gene expression of cyclin-dependent kinase inhibitors (CKIs) in white adipose tissue (WAT) and liver from high-fat fed male Wistar rats with or without vitamin C (VC) supplementation (750 mg/kg of body weight). After 56 d of experimentation, animals fed on a cafeteria diet increased significantly body weights and total body fat. Reverse transcription-polymerase chain reaction (RT-PCR) studies showed that cafeteria diet decreased p21 and p57 mRNA expression in subcutaneous WAT and increased p21 mRNA in liver. Overall, these data provide new information about the role of high fat intake on mRNA levels of several CKIs with implications in adipogenesis, cell metabolism and weight homeostasis. Interestingly, VC supplementation partially prevented diet-induced adiposity and increased p27 mRNA in liver without any changes in the other tissues and genes analyzed. Thus, hepatic mRNA changes induced by ascorbic acid indicate a possible role of these genes in diet-induced oxidative stress processes.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01
2009
2009-01-01
2013
2013-03-29
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/28191
url https://hdl.handle.net/10171/28191
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pharmaceutical Society of Japan
publisher.none.fl_str_mv Pharmaceutical Society of Japan
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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