A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection
Nanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have de...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:211125 |
| Acceso en línea: | https://ddd.uab.cat/record/211125 https://dx.doi.org/urn:doi:10.1016/j.bios.2017.05.020 |
| Access Level: | acceso abierto |
| Palabra clave: | Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
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A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetectionLópez Muñoz, Gerardo ArturoEstévez, M. Carmen|||0000-0003-3694-7186Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566Homs Corbera, AntoniGarcía-Hernandez, M. C.Imbaud, J. I.Lechuga, Laura M.|||0000-0001-5187-5358Blu-ray discPlasmonic nanostructureIntegrationMicrofluidicsBiosensingAntibodyNanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have developed a simple integrated nanoplasmonic sensor taking advantage of the periodic nanostructured array of commercial Blu-ray discs. Sensors with two gold film thicknesses (50 and 100 nm) were fabricated and optically characterized by varying the oblique-angle of the incident light in optical reflectance measurements. Contrary to the use normal light incidence previously reported with other optical discs, we observed an enhancement in sensitivity and a narrowing of the resonant linewidths as the light incidence angle was increased, which could be related to the generation of Fano resonant modes. The new sensors achieve a figure of merit (FOM) up to 35 RIU and a competitive bulk limit of detection (LOD) of 6.3×10 RIU. These values significantly improve previously reported results obtained with normal light incidence reflectance measurements using similar structures. The sensor has been combined with versatile, simple, ease to-fabricate microfluidics. The integrated chip is only 1 cm (including a PDMS flow cell with a 50 µm height microfluidic channel fabricated with double-sided adhesive tape) and all the optical components are mounted on a 10 cm×10 cm portable prototype, illustrating its facile miniaturization, integration and potential portability. Finally, to assess the label-free biosensing capability of the new sensor, we have evaluated the presence of specific antibodies against the GTF2b protein, a tumor-associate antigen (TAA) related to colorectal cancer. We have achieved a LOD in the pM order and have assessed the feasibility of directly measuring biological samples such as human serum. 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501SMURhttp://purl.org/coar/version/c_71e4c1898caa6e32info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/211125https://dx.doi.org/urn:doi:10.1016/j.bios.2017.05.020reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 TEC2016-78515-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 RTC-2014-1518-1Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2013-0295Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-624open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2111252026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| spellingShingle |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection López Muñoz, Gerardo Arturo Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
| title_short |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_full |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_fullStr |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_full_unstemmed |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_sort |
A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| dc.creator.none.fl_str_mv |
López Muñoz, Gerardo Arturo Estévez, M. Carmen|||0000-0003-3694-7186 Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Homs Corbera, Antoni García-Hernandez, M. C. Imbaud, J. I. Lechuga, Laura M.|||0000-0001-5187-5358 |
| author |
López Muñoz, Gerardo Arturo |
| author_facet |
López Muñoz, Gerardo Arturo Estévez, M. Carmen|||0000-0003-3694-7186 Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Homs Corbera, Antoni García-Hernandez, M. C. Imbaud, J. I. Lechuga, Laura M.|||0000-0001-5187-5358 |
| author_role |
author |
| author2 |
Estévez, M. Carmen|||0000-0003-3694-7186 Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Homs Corbera, Antoni García-Hernandez, M. C. Imbaud, J. I. Lechuga, Laura M.|||0000-0001-5187-5358 |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
| topic |
Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
| description |
Nanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have developed a simple integrated nanoplasmonic sensor taking advantage of the periodic nanostructured array of commercial Blu-ray discs. Sensors with two gold film thicknesses (50 and 100 nm) were fabricated and optically characterized by varying the oblique-angle of the incident light in optical reflectance measurements. Contrary to the use normal light incidence previously reported with other optical discs, we observed an enhancement in sensitivity and a narrowing of the resonant linewidths as the light incidence angle was increased, which could be related to the generation of Fano resonant modes. The new sensors achieve a figure of merit (FOM) up to 35 RIU and a competitive bulk limit of detection (LOD) of 6.3×10 RIU. These values significantly improve previously reported results obtained with normal light incidence reflectance measurements using similar structures. The sensor has been combined with versatile, simple, ease to-fabricate microfluidics. The integrated chip is only 1 cm (including a PDMS flow cell with a 50 µm height microfluidic channel fabricated with double-sided adhesive tape) and all the optical components are mounted on a 10 cm×10 cm portable prototype, illustrating its facile miniaturization, integration and potential portability. Finally, to assess the label-free biosensing capability of the new sensor, we have evaluated the presence of specific antibodies against the GTF2b protein, a tumor-associate antigen (TAA) related to colorectal cancer. We have achieved a LOD in the pM order and have assessed the feasibility of directly measuring biological samples such as human serum. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2 2017-01-01 2017 2017-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 SMUR http://purl.org/coar/version/c_71e4c1898caa6e32 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/211125 https://dx.doi.org/urn:doi:10.1016/j.bios.2017.05.020 |
| url |
https://ddd.uab.cat/record/211125 https://dx.doi.org/urn:doi:10.1016/j.bios.2017.05.020 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 TEC2016-78515-R Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RTC-2014-1518-1 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2013-0295 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-624 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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