Effective elastin-like recombinamers coating on poly(vinylidene) fluoride membranes for mesenchymal stem cell culture

[EN] Bone's inherent piezoelectricity is a key factor in regulating bone growth and mesenchymal stem cells (MSCs) fate towards the osteogenic lineage. The piezoelectric polymer poly(vinylidene) fluoride (PVDF) was thus used to manufacture electroactive membranes by means of non-solvent indu...

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Detalles Bibliográficos
Autores: Guillot-Ferriols, María Teresa, del Barrio, Ana, Costa, Carlos M., Lanceros-Méndez, Senentxu, Rodríguez-Cabello, José Carlos, Santos, Mercedes, Gómez Ribelles, José Luís|||0000-0001-9099-0885, Gallego-Ferrer, Gloria|||0000-0002-2428-0903
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/181729
Acceso en línea:https://riunet.upv.es/handle/10251/181729
Access Level:acceso abierto
Palabra clave:Poly(vinylidene) fluoride
Piezoelectricity
Elastin-like recombinamers
RGD
Layer-by-layer
Mesenchymal stem cells
MAQUINAS Y MOTORES TERMICOS
TERMODINAMICA APLICADA (UPV)
Descripción
Sumario:[EN] Bone's inherent piezoelectricity is a key factor in regulating bone growth and mesenchymal stem cells (MSCs) fate towards the osteogenic lineage. The piezoelectric polymer poly(vinylidene) fluoride (PVDF) was thus used to manufacture electroactive membranes by means of non-solvent induced phase separation (NIPS), producing porous membranes with approximately 90% of gamma-phase for MSCs culture. The combination of the porous surface and PVDF hydrophobicity hinders cell adhesion and requires a coating to improve cell culture conditions. A layer-by-layer (LbL) method was used to deposit elastin-like recombinamers (ELRs) containing RGD sequences applying click cross-linking chemistry. ELRs potential was confirmed by comparing traditional fibronectin adsorption with ELRs LbL on PVDF electroactive membranes. Porcine bone marrow MSCs preferred ELRs-coated surfaces, which enhanced initial cell adhesion and improved proliferation after 7 days. These findings lead to new possibilities for regenerative therapies in the area of bone tissue engineering, offering the advantages of MSC commitment towards the osteogenic lineage by applying electro-mechanical stimulation on electroactive substrates.

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