West Nile virus emergence in humans in Extremadura, Spain 2020

In Spain, the largest human West Nile virus (WNV) outbreak among humans was reported in 2020, constituting the second most important outbreak in Europe that season. Extremadura (southwestern Spain) was one of the affected areas, reporting six human cases. The first autochthonous human case in Spain...

Descripción completa

Detalles Bibliográficos
Autores: Macias, Alicia, Martín, Paloma, Perez-Olmeda, Mayte, Fernandez Martinez, Beatriz, Gomez-Barroso, Diana, Fernández, Esperanza, Ramos, Julián Mauro, Herrero-Romero, Laura, Rodríguez, Saray, Delgado, Elena, Sánchez-Seco, María Paz, Galán, Miguel, Corbacho, Antonio Jesús, Jimenez, Manuel, Montero-Peña, Cristian, Valle, Antonio, Vazquez, Ana
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/16343
Acceso en línea:http://hdl.handle.net/20.500.12105/16343
Access Level:acceso abierto
Palabra clave:West Nile virus
West Nile Fever
Flavivirus
Animals
Humans
Spain
Antibodies, Viral
id ES_4ea74f2824a100e0b5ea78d48efa1ef2
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/16343
network_acronym_str ES
network_name_str España
repository_id_str
spelling West Nile virus emergence in humans in Extremadura, Spain 2020Macias, AliciaMartín, PalomaPerez-Olmeda, MayteFernandez Martinez, BeatrizGomez-Barroso, DianaFernández, EsperanzaRamos, Julián MauroHerrero-Romero, LauraRodríguez, SarayDelgado, ElenaSánchez-Seco, María PazGalán, MiguelCorbacho, Antonio JesúsJimenez, ManuelMontero-Peña, CristianValle, AntonioVazquez, AnaWest Nile virusWest Nile FeverFlavivirusAnimalsHumansSpainAntibodies, ViralIn Spain, the largest human West Nile virus (WNV) outbreak among humans was reported in 2020, constituting the second most important outbreak in Europe that season. Extremadura (southwestern Spain) was one of the affected areas, reporting six human cases. The first autochthonous human case in Spain was reported in Extremadura in 2004, and no other human cases were reported until 2020. In this work, we describe the first WNV human outbreak registered in Extremadura, focusing on the most important clinical aspects, diagnostic results, and control actions which followed. In 2020, from September to October, human WNV infections were diagnosed using a combination of molecular and serological methods (an in-house specific qRT-PCR and a commercial ELISA for anti-WNV IgM and IgG antibodies) and by analysing serum, urine, and/or cerebrospinal fluid samples. Serological positive serum samples were further tested using commercial kits against related flaviviruses Usutu and Tick-borne encephalitis in order to analyse serological reactivity and to confirm the results by neutralisation assays. In total, six cases of WNV infection (five with neuroinvasive disease and one with fever) were identified. Clinical presentation and laboratory findings are described. No viral RNA was detected in any of the analysed samples, but serological cross-reactivity was detected against the other tested flaviviruses. Molecular and serological methods for WNV detection in various samples as well as differential diagnosis are recommended. The largest number of human cases of WNV infection ever registered in Extremadura, Spain, occurred in 2020 in areas where circulation of WNV and other flaviviruses has been previously reported in humans and animals. Therefore, it is necessary to enhance surveillance not only for the early detection and implementation of response measures for WNV but also for other emerging flaviviruses that could be endemic in this area.Frontiers MediaInstituto de Salud Carlos III20232023-08-2520232023-07-0120232023-07-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/16343reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/163432026-06-12T12:43:37Z
dc.title.none.fl_str_mv West Nile virus emergence in humans in Extremadura, Spain 2020
title West Nile virus emergence in humans in Extremadura, Spain 2020
spellingShingle West Nile virus emergence in humans in Extremadura, Spain 2020
Macias, Alicia
West Nile virus
West Nile Fever
Flavivirus
Animals
Humans
Spain
Antibodies, Viral
title_short West Nile virus emergence in humans in Extremadura, Spain 2020
title_full West Nile virus emergence in humans in Extremadura, Spain 2020
title_fullStr West Nile virus emergence in humans in Extremadura, Spain 2020
title_full_unstemmed West Nile virus emergence in humans in Extremadura, Spain 2020
title_sort West Nile virus emergence in humans in Extremadura, Spain 2020
dc.creator.none.fl_str_mv Macias, Alicia
Martín, Paloma
Perez-Olmeda, Mayte
Fernandez Martinez, Beatriz
Gomez-Barroso, Diana
Fernández, Esperanza
Ramos, Julián Mauro
Herrero-Romero, Laura
Rodríguez, Saray
Delgado, Elena
Sánchez-Seco, María Paz
Galán, Miguel
Corbacho, Antonio Jesús
Jimenez, Manuel
Montero-Peña, Cristian
Valle, Antonio
Vazquez, Ana
author Macias, Alicia
author_facet Macias, Alicia
Martín, Paloma
Perez-Olmeda, Mayte
Fernandez Martinez, Beatriz
Gomez-Barroso, Diana
Fernández, Esperanza
Ramos, Julián Mauro
Herrero-Romero, Laura
Rodríguez, Saray
Delgado, Elena
Sánchez-Seco, María Paz
Galán, Miguel
Corbacho, Antonio Jesús
Jimenez, Manuel
Montero-Peña, Cristian
Valle, Antonio
Vazquez, Ana
author_role author
author2 Martín, Paloma
Perez-Olmeda, Mayte
Fernandez Martinez, Beatriz
Gomez-Barroso, Diana
Fernández, Esperanza
Ramos, Julián Mauro
Herrero-Romero, Laura
Rodríguez, Saray
Delgado, Elena
Sánchez-Seco, María Paz
Galán, Miguel
Corbacho, Antonio Jesús
Jimenez, Manuel
Montero-Peña, Cristian
Valle, Antonio
Vazquez, Ana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
dc.subject.none.fl_str_mv West Nile virus
West Nile Fever
Flavivirus
Animals
Humans
Spain
Antibodies, Viral
topic West Nile virus
West Nile Fever
Flavivirus
Animals
Humans
Spain
Antibodies, Viral
description In Spain, the largest human West Nile virus (WNV) outbreak among humans was reported in 2020, constituting the second most important outbreak in Europe that season. Extremadura (southwestern Spain) was one of the affected areas, reporting six human cases. The first autochthonous human case in Spain was reported in Extremadura in 2004, and no other human cases were reported until 2020. In this work, we describe the first WNV human outbreak registered in Extremadura, focusing on the most important clinical aspects, diagnostic results, and control actions which followed. In 2020, from September to October, human WNV infections were diagnosed using a combination of molecular and serological methods (an in-house specific qRT-PCR and a commercial ELISA for anti-WNV IgM and IgG antibodies) and by analysing serum, urine, and/or cerebrospinal fluid samples. Serological positive serum samples were further tested using commercial kits against related flaviviruses Usutu and Tick-borne encephalitis in order to analyse serological reactivity and to confirm the results by neutralisation assays. In total, six cases of WNV infection (five with neuroinvasive disease and one with fever) were identified. Clinical presentation and laboratory findings are described. No viral RNA was detected in any of the analysed samples, but serological cross-reactivity was detected against the other tested flaviviruses. Molecular and serological methods for WNV detection in various samples as well as differential diagnosis are recommended. The largest number of human cases of WNV infection ever registered in Extremadura, Spain, occurred in 2020 in areas where circulation of WNV and other flaviviruses has been previously reported in humans and animals. Therefore, it is necessary to enhance surveillance not only for the early detection and implementation of response measures for WNV but also for other emerging flaviviruses that could be endemic in this area.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-08-25
2023
2023-07-01
2023
2023-07-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/16343
url http://hdl.handle.net/20.500.12105/16343
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407770170621953
score 15,81155

Similares en LA Referencia