Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses

Drought and salinity can be serious problems for agricultural productivity in certain planet areas. Leptosphaeria maculans is the causative agent of the blackleg in crucifer plants. In this work, a novel methodology for studying the effects of fungal metabolites (volatile and non-volatile) on plant...

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Autor: Poveda Arias, Jorge
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/42645
Acceso en línea:https://hdl.handle.net/2454/42645
Access Level:acceso abierto
Palabra clave:Calli
Drought
Leptosphaeria maculans
Metabolites
Salinity
Tomato
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spelling Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stressesPoveda Arias, JorgeCalliDroughtLeptosphaeria maculansMetabolitesSalinityTomatoDrought and salinity can be serious problems for agricultural productivity in certain planet areas. Leptosphaeria maculans is the causative agent of the blackleg in crucifer plants. In this work, a novel methodology for studying the effects of fungal metabolites (volatile and non-volatile) on plant calli in the presence of abiotic stresses is presented, by using L. maculans, tomato calli, and drought and salinity stresses. In this way, this study has reported how, under salinity and drought stresses, the growth and vitality of tomato calli is inhibited, increasing its tissues-oxidation and accumulation of ROS. By applying metabolites from L. maculans, the growth of calli treated with non-volatile metabolites showed and increment under salinity and drought conditions. On the other hand, calli treated with volatile metabolites showed an increment in tissues-vitality under salinity and drought conditions. A series of gene expression analysis was also conducted in order to determine the performance of related genes. Results of this study showed that growth related gene expression was induced, together with abiotic stress tolerance gene in response to abscisic acid, AREB1. In addition, the application of volatile metabolites from L. maculans on tomato calli without abiotic stresses increases its growth and vitality, and reduces its oxidation and accumulation of ROS, in accordance with the results of gene expression obtained. The ability of L. maculans metabolites to increase plant tolerance to abiotic stresses could be related to their ability to produce volatile and non-volatile-metabolites, which induce the antioxidant enzyme activity or accumulation of antioxidant compounds, or their ability to increase the expression of ABA-dependent response genes to abiotic stresses.This work was supported by the Instituto de Estudios Zamoranos ‘Florián de Ocampo’ (IEZFO), in the XXVII and XXVIII Call for Research Grants, corresponding to the years 2019 and 2020.Global Science BooksInstitute for Multidisciplinary Research in Applied Biology - IMAB2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/42645reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2021 The Author(s). Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/426452026-06-17T12:41:47Z
dc.title.none.fl_str_mv Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
title Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
spellingShingle Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
Poveda Arias, Jorge
Calli
Drought
Leptosphaeria maculans
Metabolites
Salinity
Tomato
title_short Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
title_full Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
title_fullStr Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
title_full_unstemmed Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
title_sort Effect of volatile and non-volatile metabolites from Leptosphaeria maculans on tomato calli under abiotic stresses
dc.creator.none.fl_str_mv Poveda Arias, Jorge
author Poveda Arias, Jorge
author_facet Poveda Arias, Jorge
author_role author
dc.contributor.none.fl_str_mv Institute for Multidisciplinary Research in Applied Biology - IMAB
dc.subject.none.fl_str_mv Calli
Drought
Leptosphaeria maculans
Metabolites
Salinity
Tomato
topic Calli
Drought
Leptosphaeria maculans
Metabolites
Salinity
Tomato
description Drought and salinity can be serious problems for agricultural productivity in certain planet areas. Leptosphaeria maculans is the causative agent of the blackleg in crucifer plants. In this work, a novel methodology for studying the effects of fungal metabolites (volatile and non-volatile) on plant calli in the presence of abiotic stresses is presented, by using L. maculans, tomato calli, and drought and salinity stresses. In this way, this study has reported how, under salinity and drought stresses, the growth and vitality of tomato calli is inhibited, increasing its tissues-oxidation and accumulation of ROS. By applying metabolites from L. maculans, the growth of calli treated with non-volatile metabolites showed and increment under salinity and drought conditions. On the other hand, calli treated with volatile metabolites showed an increment in tissues-vitality under salinity and drought conditions. A series of gene expression analysis was also conducted in order to determine the performance of related genes. Results of this study showed that growth related gene expression was induced, together with abiotic stress tolerance gene in response to abscisic acid, AREB1. In addition, the application of volatile metabolites from L. maculans on tomato calli without abiotic stresses increases its growth and vitality, and reduces its oxidation and accumulation of ROS, in accordance with the results of gene expression obtained. The ability of L. maculans metabolites to increase plant tolerance to abiotic stresses could be related to their ability to produce volatile and non-volatile-metabolites, which induce the antioxidant enzyme activity or accumulation of antioxidant compounds, or their ability to increase the expression of ABA-dependent response genes to abiotic stresses.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/42645
url https://hdl.handle.net/2454/42645
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2021 The Author(s). Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2021 The Author(s). Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Global Science Books
publisher.none.fl_str_mv Global Science Books
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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