Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus

Transjugation is an unconventional conjugation mechanism in Thermus thermophilus (Tth) that involves the active participation of both mating partners, encompassing a DNA secretion system (DSS) in the donor and an active natural competence apparatus (NCA) in the recipient cells. DSS is encoded within...

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Autores: Blesa Esteban, Alba Mercedes, Berenguer Carlos, José, Mencía, Mario, González de la Fuente, Sara, Baquedano, Ignacio
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/95956
Acceso en línea:https://hdl.handle.net/20.500.14352/95956
Access Level:acceso abierto
Palabra clave:ICE
conjugation
mosaicity
pangenome
Thermus
Ciencias Biomédicas
24 Ciencias de la Vida
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spelling Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilusBlesa Esteban, Alba MercedesBerenguer Carlos, JoséMencía, MarioGonzález de la Fuente, SaraBaquedano, IgnacioICEconjugationmosaicitypangenomeThermusCiencias Biomédicas24 Ciencias de la VidaTransjugation is an unconventional conjugation mechanism in Thermus thermophilus (Tth) that involves the active participation of both mating partners, encompassing a DNA secretion system (DSS) in the donor and an active natural competence apparatus (NCA) in the recipient cells. DSS is encoded within an integrative and conjugative element (ICETh1) in the strain Tth HB27, whereas the NCA is constitutively expressed in both mates. Previous experiments suggested the presence of multiple origins of transfer along the genome, which could generate genomic mosaicity among the progeny. Here, we designed transjugation experiments between two closely related strains of Tth with highly syntenic genomes, containing enough single nucleotide polymorphisms to allow precise parenthood analysis. Individual clones from the progeny were sequenced, revealing their origin as derivatives of our ICETh1-containing intended “donor” strain (HB27), which had acquired separate fragments from the genome of the ICETh1-free HB8 cells, which are our intended recipient. Due to the bidirectional nature of transjugation, only assays employing competence-defective HB27 derivatives as donors allowed the recovery of HB8-derived progeny. These results show a preference for a retrotransfer mechanism in transjugation in ICETh1-bearing strains, supporting an inter-strain gene-capture function for ICETh1. This function could benefit the donor-capable host by facilitating the acquisition of adaptive traits from external sources, ultimately increasing the open pangenome of Thermus, maximizing the potential repertoire of physiological and phenotypical traits related to adaptation and speciationBerenguer Carlos, JoséMDPIUniversidad Complutense de Madrid20202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/95956reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengBIO2016-77031-R Not available Not availablePID2019-109073RB-I00 Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/959562026-06-02T12:44:21Z
dc.title.none.fl_str_mv Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
title Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
spellingShingle Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
Blesa Esteban, Alba Mercedes
ICE
conjugation
mosaicity
pangenome
Thermus
Ciencias Biomédicas
24 Ciencias de la Vida
title_short Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
title_full Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
title_fullStr Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
title_full_unstemmed Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
title_sort Integrative and Conjugative Element ICETh1 functions as a pangenomic DNA capture module in Thermus thermophilus
dc.creator.none.fl_str_mv Blesa Esteban, Alba Mercedes
Berenguer Carlos, José
Mencía, Mario
González de la Fuente, Sara
Baquedano, Ignacio
author Blesa Esteban, Alba Mercedes
author_facet Blesa Esteban, Alba Mercedes
Berenguer Carlos, José
Mencía, Mario
González de la Fuente, Sara
Baquedano, Ignacio
author_role author
author2 Berenguer Carlos, José
Mencía, Mario
González de la Fuente, Sara
Baquedano, Ignacio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Berenguer Carlos, José
MDPI
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv ICE
conjugation
mosaicity
pangenome
Thermus
Ciencias Biomédicas
24 Ciencias de la Vida
topic ICE
conjugation
mosaicity
pangenome
Thermus
Ciencias Biomédicas
24 Ciencias de la Vida
description Transjugation is an unconventional conjugation mechanism in Thermus thermophilus (Tth) that involves the active participation of both mating partners, encompassing a DNA secretion system (DSS) in the donor and an active natural competence apparatus (NCA) in the recipient cells. DSS is encoded within an integrative and conjugative element (ICETh1) in the strain Tth HB27, whereas the NCA is constitutively expressed in both mates. Previous experiments suggested the presence of multiple origins of transfer along the genome, which could generate genomic mosaicity among the progeny. Here, we designed transjugation experiments between two closely related strains of Tth with highly syntenic genomes, containing enough single nucleotide polymorphisms to allow precise parenthood analysis. Individual clones from the progeny were sequenced, revealing their origin as derivatives of our ICETh1-containing intended “donor” strain (HB27), which had acquired separate fragments from the genome of the ICETh1-free HB8 cells, which are our intended recipient. Due to the bidirectional nature of transjugation, only assays employing competence-defective HB27 derivatives as donors allowed the recovery of HB8-derived progeny. These results show a preference for a retrotransfer mechanism in transjugation in ICETh1-bearing strains, supporting an inter-strain gene-capture function for ICETh1. This function could benefit the donor-capable host by facilitating the acquisition of adaptive traits from external sources, ultimately increasing the open pangenome of Thermus, maximizing the potential repertoire of physiological and phenotypical traits related to adaptation and speciation
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/95956
url https://hdl.handle.net/20.500.14352/95956
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv BIO2016-77031-R Not available Not available
PID2019-109073RB-I00 Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407768540086272
score 15.300719

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