In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay

Recently, mineral healing clays have gained much attention for wound-dressing applications. Here, we selected halloysite (HAL) clay as a biocompatible, non-toxic material that is useful as a drug delivery system to enhance the healing properties of water-soluble terpenoids 1-3 (T1-3). Terpenoids-loa...

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Autores: Marinelli , Lisa, Cacciatore, Ivana, Eusepi, Piera, Dimmito, Marilisa Pia, Di Rienzo, Annalisa, Reale, Marcella, Costantini, Erica, Borrego Sánchez, Ana, García-Villén, Fátima, Viseras, César, Morroni, Gianluca, Fioriti, Simona, Brescini, Lucia, Di Stefano, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/278124
Acceso en línea:http://hdl.handle.net/10261/278124
Access Level:acceso abierto
Palabra clave:Clay
Halloysite
Skin regeneration
Terpenoids
Wound healing
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spelling In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clayMarinelli , LisaCacciatore, IvanaEusepi, PieraDimmito, Marilisa PiaDi Rienzo, AnnalisaReale, MarcellaCostantini, EricaBorrego Sánchez, AnaGarcía-Villén, FátimaViseras, CésarMorroni, GianlucaFioriti, SimonaBrescini, LuciaDi Stefano, AntonioClayHalloysiteSkin regenerationTerpenoidsWound healingRecently, mineral healing clays have gained much attention for wound-dressing applications. Here, we selected halloysite (HAL) clay as a biocompatible, non-toxic material that is useful as a drug delivery system to enhance the healing properties of water-soluble terpenoids 1-3 (T1-3). Terpenoids-loaded HAL clay (TH1-3) was prepared and characterized by adsorption equilibrium studies, X-ray powder diffraction (XRPD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, and release studies. The results reveal that T1-3 were adsorbed at the HAL surface with good efficiency. The prevalent mechanism of drug retention is due to the adsorption via electrostatic interactions between the cationic groups of the T1-3 and the HAL’s external surface. Release studies demonstrated that T3 was released in a higher percentage (>60%) compared to T1-2 (≈50%). Additionally, TH1-3 were assessed for their antimicrobial activity and capability to promote the re-epithelialization of scratched HaCat monolayers, through the time-kill test and the wound-healing assays, respectively. The results reveal that all the tested formulations were able to reduce the microbial growth after 1 h of incubation and that they ensured complete wound closure after 48 h. Furthermore, at the concentration of 1 µg/mL, TH3 exhibited 45% wound closure at 24 h, compared to TH1 (27%) and TH2 (30%), proving to be the best candidate in making the tissue-repair process easier and faster.This research was funded by the Italian Ministry of Education, University and Research (University “G. d’Annunzio” of Chieti-Pescara), grant number FAR 2020.Multidisciplinary Digital Publishing InstituteMinistero dell'Istruzione, dell'Università e della RicercaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/278124reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/pharmaceutics13081117Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2781242026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
title In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
spellingShingle In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
Marinelli , Lisa
Clay
Halloysite
Skin regeneration
Terpenoids
Wound healing
title_short In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
title_full In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
title_fullStr In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
title_full_unstemmed In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
title_sort In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay
dc.creator.none.fl_str_mv Marinelli , Lisa
Cacciatore, Ivana
Eusepi, Piera
Dimmito, Marilisa Pia
Di Rienzo, Annalisa
Reale, Marcella
Costantini, Erica
Borrego Sánchez, Ana
García-Villén, Fátima
Viseras, César
Morroni, Gianluca
Fioriti, Simona
Brescini, Lucia
Di Stefano, Antonio
author Marinelli , Lisa
author_facet Marinelli , Lisa
Cacciatore, Ivana
Eusepi, Piera
Dimmito, Marilisa Pia
Di Rienzo, Annalisa
Reale, Marcella
Costantini, Erica
Borrego Sánchez, Ana
García-Villén, Fátima
Viseras, César
Morroni, Gianluca
Fioriti, Simona
Brescini, Lucia
Di Stefano, Antonio
author_role author
author2 Cacciatore, Ivana
Eusepi, Piera
Dimmito, Marilisa Pia
Di Rienzo, Annalisa
Reale, Marcella
Costantini, Erica
Borrego Sánchez, Ana
García-Villén, Fátima
Viseras, César
Morroni, Gianluca
Fioriti, Simona
Brescini, Lucia
Di Stefano, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministero dell'Istruzione, dell'Università e della Ricerca
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Clay
Halloysite
Skin regeneration
Terpenoids
Wound healing
topic Clay
Halloysite
Skin regeneration
Terpenoids
Wound healing
description Recently, mineral healing clays have gained much attention for wound-dressing applications. Here, we selected halloysite (HAL) clay as a biocompatible, non-toxic material that is useful as a drug delivery system to enhance the healing properties of water-soluble terpenoids 1-3 (T1-3). Terpenoids-loaded HAL clay (TH1-3) was prepared and characterized by adsorption equilibrium studies, X-ray powder diffraction (XRPD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, and release studies. The results reveal that T1-3 were adsorbed at the HAL surface with good efficiency. The prevalent mechanism of drug retention is due to the adsorption via electrostatic interactions between the cationic groups of the T1-3 and the HAL’s external surface. Release studies demonstrated that T3 was released in a higher percentage (>60%) compared to T1-2 (≈50%). Additionally, TH1-3 were assessed for their antimicrobial activity and capability to promote the re-epithelialization of scratched HaCat monolayers, through the time-kill test and the wound-healing assays, respectively. The results reveal that all the tested formulations were able to reduce the microbial growth after 1 h of incubation and that they ensured complete wound closure after 48 h. Furthermore, at the concentration of 1 µg/mL, TH3 exhibited 45% wound closure at 24 h, compared to TH1 (27%) and TH2 (30%), proving to be the best candidate in making the tissue-repair process easier and faster.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/278124
url http://hdl.handle.net/10261/278124
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/pharmaceutics13081117
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407766159818752
score 15.81155

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