CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells

D-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glio...

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Detalhes bibliográficos
Autores: Fuentes-Baile, M, Bello-Gil, D, Perez-Valenciano, E, Sanz, JM, Garcia-Morales, P, Maestro, B, Ventero, MP, Alenda, C, Barbera, VM, Saceda, M
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2020
País:España
Recursos:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
Repositório:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
OAI Identifier:oai:isabial.fundanetsuite.com:p6012
Acesso em linha:https://isabial.portalinvestigacion.com/publicaciones6012
Access Level:Acceso aberto
Palavra-chave:magnetic nanoparticle
cancer therapy
reactive oxygen species
oxidative damage
cytotoxicity
choline-binding proteins
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spelling CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer CellsFuentes-Baile, MBello-Gil, DPerez-Valenciano, ESanz, JMGarcia-Morales, PMaestro, BVentero, MPAlenda, CBarbera, VMSaceda, Mmagnetic nanoparticlecancer therapyreactive oxygen speciesoxidative damagecytotoxicitycholine-binding proteinsD-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glioblastoma cell lines. We found that the enzyme induces cell death in most of the cell lines tested and its efficiency increases significantly when it is immobilized in nanoparticles. We also tested this enzyme therapy in non-tumor cells, and we found that there is not cell death induction, or it is significantly lower than in tumor cells. The mechanism triggering cell death is apparently a classical apoptosis pathway in the glioblastoma cell lines, while in colon and pancreatic carcinoma cell lines, CLytA-DAAO-induced cell death is a necrosis. Our results constitute a proof of concept that an enzymatic therapy, based on magnetic nanoparticles-delivering CLytA-DAAO, could constitute a useful therapy against cancer and besides it could be used as an enhancer of other treatments such as epigenetic therapy, radiotherapy, and treatments based on DNA repair.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://isabial.portalinvestigacion.com/publicaciones6012BiomoleculesISSN: 2218273Xreponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicanteinstname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)Inglésinfo:eu-repo/semantics/openAccessoai:isabial.fundanetsuite.com:p60122026-06-12T10:20:37Z
dc.title.none.fl_str_mv CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
title CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
spellingShingle CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
Fuentes-Baile, M
magnetic nanoparticle
cancer therapy
reactive oxygen species
oxidative damage
cytotoxicity
choline-binding proteins
title_short CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
title_full CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
title_fullStr CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
title_full_unstemmed CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
title_sort CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells
dc.creator.none.fl_str_mv Fuentes-Baile, M
Bello-Gil, D
Perez-Valenciano, E
Sanz, JM
Garcia-Morales, P
Maestro, B
Ventero, MP
Alenda, C
Barbera, VM
Saceda, M
author Fuentes-Baile, M
author_facet Fuentes-Baile, M
Bello-Gil, D
Perez-Valenciano, E
Sanz, JM
Garcia-Morales, P
Maestro, B
Ventero, MP
Alenda, C
Barbera, VM
Saceda, M
author_role author
author2 Bello-Gil, D
Perez-Valenciano, E
Sanz, JM
Garcia-Morales, P
Maestro, B
Ventero, MP
Alenda, C
Barbera, VM
Saceda, M
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv magnetic nanoparticle
cancer therapy
reactive oxygen species
oxidative damage
cytotoxicity
choline-binding proteins
topic magnetic nanoparticle
cancer therapy
reactive oxygen species
oxidative damage
cytotoxicity
choline-binding proteins
description D-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glioblastoma cell lines. We found that the enzyme induces cell death in most of the cell lines tested and its efficiency increases significantly when it is immobilized in nanoparticles. We also tested this enzyme therapy in non-tumor cells, and we found that there is not cell death induction, or it is significantly lower than in tumor cells. The mechanism triggering cell death is apparently a classical apoptosis pathway in the glioblastoma cell lines, while in colon and pancreatic carcinoma cell lines, CLytA-DAAO-induced cell death is a necrosis. Our results constitute a proof of concept that an enzymatic therapy, based on magnetic nanoparticles-delivering CLytA-DAAO, could constitute a useful therapy against cancer and besides it could be used as an enhancer of other treatments such as epigenetic therapy, radiotherapy, and treatments based on DNA repair.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://isabial.portalinvestigacion.com/publicaciones6012
url https://isabial.portalinvestigacion.com/publicaciones6012
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Biomolecules
ISSN: 2218273X
reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
instname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
instname_str Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
reponame_str r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
collection r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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