Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain

Chlamydia trachomatis; Sexually transmitted infection; Treatment failure

Detalles Bibliográficos
Autores: Boga, Jose Antonio, Vazquez, Fernando, Milagro Beamonte , Ana, Vall-Mayans, Marti, VILLA, LAURA, Otero, Luis, Salmeron, Paula
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Departament de Salut de la Generalitat de Catalunya (DS)
Repositorio:Scientia. Dipòsit d'Informació Digital del Departament de Salut
OAI Identifier:oai:scientiasalut.gencat.cat:11351/9985
Acceso en línea:https://hdl.handle.net/11351/9985
Access Level:acceso abierto
Palabra clave:Medicaments antiinfecciosos - Efectes secundaris
Malalties de transmissió sexual - Tractament
DISEASES::Bacterial Infections and Mycoses::Infection::Sexually Transmitted Diseases
Other subheadings::Other subheadings::Other subheadings::/drug therapy
CHEMICALS AND DRUGS::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents
Other subheadings::Other subheadings::Other subheadings::/adverse effects
ENFERMEDADES::infecciones bacterianas y micosis::infección::enfermedades de transmisión sexual
Otros calificadores::Otros calificadores::Otros calificadores::/farmacoterapia
COMPUESTOS QUÍMICOS Y DROGAS::acciones y usos químicos::acciones farmacológicas::usos terapéuticos::antiinfecciosos
Otros calificadores::Otros calificadores::Otros calificadores::/efectos adversos
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oai_identifier_str oai:scientiasalut.gencat.cat:11351/9985
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
title Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
spellingShingle Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
Boga, Jose Antonio
Medicaments antiinfecciosos - Efectes secundaris
Malalties de transmissió sexual - Tractament
DISEASES::Bacterial Infections and Mycoses::Infection::Sexually Transmitted Diseases
Other subheadings::Other subheadings::Other subheadings::/drug therapy
CHEMICALS AND DRUGS::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents
Other subheadings::Other subheadings::Other subheadings::/adverse effects
ENFERMEDADES::infecciones bacterianas y micosis::infección::enfermedades de transmisión sexual
Otros calificadores::Otros calificadores::Otros calificadores::/farmacoterapia
COMPUESTOS QUÍMICOS Y DROGAS::acciones y usos químicos::acciones farmacológicas::usos terapéuticos::antiinfecciosos
Otros calificadores::Otros calificadores::Otros calificadores::/efectos adversos
title_short Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
title_full Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
title_fullStr Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
title_full_unstemmed Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
title_sort Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain
dc.creator.none.fl_str_mv Boga, Jose Antonio
Vazquez, Fernando
Milagro Beamonte , Ana
Vall-Mayans, Marti
VILLA, LAURA
Otero, Luis
Salmeron, Paula
author Boga, Jose Antonio
author_facet Boga, Jose Antonio
Vazquez, Fernando
Milagro Beamonte , Ana
Vall-Mayans, Marti
VILLA, LAURA
Otero, Luis
Salmeron, Paula
author_role author
author2 Vazquez, Fernando
Milagro Beamonte , Ana
Vall-Mayans, Marti
VILLA, LAURA
Otero, Luis
Salmeron, Paula
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Villa L] Microbiology Department, Central University Hospital of Asturias and Health Research Institute of Asturias (ISPA), Oviedo, Spain. Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. [Boga JA] Microbiology Department, Central University Hospital of Asturias and Health Research Institute of Asturias (ISPA), Oviedo, Spain. [Otero L] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Microbiology Department, Cabueñes University Hospital, and Health Research Institute of Asturias (ISPA), Gijón, Spain. [Vazquez F] Microbiology Department, Central University Hospital of Asturias and Health Research Institute of Asturias (ISPA), Oviedo, Spain. Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Department of Functional Biology, Microbiology Area, Faculty of Medicine, University of Oviedo, Oviedo, Spain. [Milagro A] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Microbiology Department, Miguel Servet University Hospital, Zaragoza, Spain. [Salmerón P] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Vall-Mayans M] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Unitat d’Infeccions de Transmissió Sexual Drassanes-Vall d'Hebron Hospital Universitari, Barcelona, Spain. Servei de Malalties Infeccioses, Vall d’Hebron Hospital Universitari, Barcelona, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Medicaments antiinfecciosos - Efectes secundaris
Malalties de transmissió sexual - Tractament
DISEASES::Bacterial Infections and Mycoses::Infection::Sexually Transmitted Diseases
Other subheadings::Other subheadings::Other subheadings::/drug therapy
CHEMICALS AND DRUGS::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents
Other subheadings::Other subheadings::Other subheadings::/adverse effects
ENFERMEDADES::infecciones bacterianas y micosis::infección::enfermedades de transmisión sexual
Otros calificadores::Otros calificadores::Otros calificadores::/farmacoterapia
COMPUESTOS QUÍMICOS Y DROGAS::acciones y usos químicos::acciones farmacológicas::usos terapéuticos::antiinfecciosos
Otros calificadores::Otros calificadores::Otros calificadores::/efectos adversos
topic Medicaments antiinfecciosos - Efectes secundaris
Malalties de transmissió sexual - Tractament
DISEASES::Bacterial Infections and Mycoses::Infection::Sexually Transmitted Diseases
Other subheadings::Other subheadings::Other subheadings::/drug therapy
CHEMICALS AND DRUGS::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents
Other subheadings::Other subheadings::Other subheadings::/adverse effects
ENFERMEDADES::infecciones bacterianas y micosis::infección::enfermedades de transmisión sexual
Otros calificadores::Otros calificadores::Otros calificadores::/farmacoterapia
COMPUESTOS QUÍMICOS Y DROGAS::acciones y usos químicos::acciones farmacológicas::usos terapéuticos::antiinfecciosos
Otros calificadores::Otros calificadores::Otros calificadores::/efectos adversos
description Chlamydia trachomatis; Sexually transmitted infection; Treatment failure
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/9985
url https://hdl.handle.net/11351/9985
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Antibiotics;12(6)
https://doi.org/10.3390/antibiotics12060975
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Scientia
reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut
instname:Departament de Salut de la Generalitat de Catalunya (DS)
instname_str Departament de Salut de la Generalitat de Catalunya (DS)
reponame_str Scientia. Dipòsit d'Informació Digital del Departament de Salut
collection Scientia. Dipòsit d'Informació Digital del Departament de Salut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in SpainBoga, Jose AntonioVazquez, FernandoMilagro Beamonte , Ana Vall-Mayans, MartiVILLA, LAURAOtero, LuisSalmeron, PaulaMedicaments antiinfecciosos - Efectes secundarisMalalties de transmissió sexual - TractamentDISEASES::Bacterial Infections and Mycoses::Infection::Sexually Transmitted DiseasesOther subheadings::Other subheadings::Other subheadings::/drug therapyCHEMICALS AND DRUGS::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective AgentsOther subheadings::Other subheadings::Other subheadings::/adverse effectsENFERMEDADES::infecciones bacterianas y micosis::infección::enfermedades de transmisión sexualOtros calificadores::Otros calificadores::Otros calificadores::/farmacoterapiaCOMPUESTOS QUÍMICOS Y DROGAS::acciones y usos químicos::acciones farmacológicas::usos terapéuticos::antiinfecciososOtros calificadores::Otros calificadores::Otros calificadores::/efectos adversosChlamydia trachomatis; Sexually transmitted infection; Treatment failureChlamydia trachomatis; Infecció de transmissió sexual; Fracàs del tractamentChlamydia trachomatis; Infección transmitida sexualmente; Fracaso del tratamientoThe aim of this multicentre project (seven hospitals across the Spanish National Health Service) was to study the phenotypic and genotypic susceptibility of C. trachomatis to the main antimicrobials used (macrolides, doxycycline, and quinolones) in isolates from patients with clinical treatment failure in whom reinfection had been ruled out. During 2018–2019, 73 clinical isolates were selected. Sixty-nine clinical specimens were inoculated onto confluent McCoy cell monolayers for phenotypic susceptibility testing. The minimum inhibitory concentration for azithromycin and doxycycline was defined as the lowest concentration associated with an at least 95% reduction in inclusion-forming units after one passage in the presence of the antibiotic compared to the initial inoculum for each strain (control). Sequencing analysis was performed for the genotypic detection of resistance to macrolides, analysing mutations in the 23S rRNA gene (at positions 2057, 2058, 2059, and 2611), and quinolones, analysing a fragment of the gyrA gene, and searching for the G248T mutation (Ser83->Ile). For tetracyclines, in-house RT-PCR was used to test for the tet(C) gene. The phenotypic susceptibility testing was successful for 10 isolates. All the isolates had minimum inhibitory concentrations for azithromycin ≤ 0.125 mg/L and for doxycycline ≤ 0.064 mg/L and were considered sensitive. Of the 73 strains studied, no mutations were found at positions T2611C or G248T of the gyrA gene. We successfully sequenced 66 isolates. No macrolide resistance-associated mutations were found at positions 2057, 2058, 2059, or T2611C. None of the isolates carried the tet(C) gene. We found no evidence for genomic resistance in this large, clinically relevant dataset.This study was funded by Instituto de Salud Carlos III (ISCIII) via the project “PI17/01886” and co-funded by the European Union.MDPIInstitut Català de la Salut[Villa L] Microbiology Department, Central University Hospital of Asturias and Health Research Institute of Asturias (ISPA), Oviedo, Spain. Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. [Boga JA] Microbiology Department, Central University Hospital of Asturias and Health Research Institute of Asturias (ISPA), Oviedo, Spain. [Otero L] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Microbiology Department, Cabueñes University Hospital, and Health Research Institute of Asturias (ISPA), Gijón, Spain. [Vazquez F] Microbiology Department, Central University Hospital of Asturias and Health Research Institute of Asturias (ISPA), Oviedo, Spain. Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Department of Functional Biology, Microbiology Area, Faculty of Medicine, University of Oviedo, Oviedo, Spain. [Milagro A] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Microbiology Department, Miguel Servet University Hospital, Zaragoza, Spain. [Salmerón P] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Vall-Mayans M] Sexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), Madrid, Spain. Unitat d’Infeccions de Transmissió Sexual Drassanes-Vall d'Hebron Hospital Universitari, Barcelona, Spain. Servei de Malalties Infeccioses, Vall d’Hebron Hospital Universitari, Barcelona, SpainVall d'Hebron Barcelona Hospital Campus202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttps://hdl.handle.net/11351/9985Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésAntibiotics;12(6)https://doi.org/10.3390/antibiotics12060975Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:scientiasalut.gencat.cat:11351/99852026-06-12T09:38:37Z
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